Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1983 Mar;48(3):423–431.

Effect of passively administered isologous anti-idiotypes directed against anti-carrier (ovalbumin) antibodies on the anti-hapten IgE and IgG antibody responses in BALB/c mice.

K Blaser, T Nakagawa, A L De Weck
PMCID: PMC1454049  PMID: 6600704

Abstract

Recently we have shown that active immunization of syngenic animals with anti-ovalbumin antibodies evokes an anti-idiotypic (aId) response, which in consequence leads to suppression of the anti-hapten (benzylpenicilloyl, BPO; dinitrophenyl, DNP) IgE and IgG formation subsequently attempted by immunization with low doses of hapten-OVA conjugates. In this work we describe attempts to suppress a primary or an already established anti-hapten IgE response by passive administration of (anti-carrier) anti-idiotypes to BALB/c mice. Our results show that ongoing anti-BPO or anti-DNP IgE responses can be depressed by injection of (anti-ovalbumin) aId, provided mice were previously immunized with conjugates of the haptens with the ovalbumin (OVA) carrier. The same animals suppressed for IgE also produce less anti-hapten and anti-carrier IgG antibodies but only after 5-6 weeks following the aId injection. The primary IgE response could be blocked by treating mice with (anti-OVA) aId and antigen at the same time.

Full text

PDF
424

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blaser K., Geiser M., de Weck A. L. Suppression of phosphorylcholine-specific IgE antibody formation in BALB/c mice by isologous anti-T 15 antiserum. Eur J Immunol. 1979 Dec;9(12):1017–1020. doi: 10.1002/eji.1830091218. [DOI] [PubMed] [Google Scholar]
  2. Blaser K., Nakagawa T., de Weck A. L. Suppression of anti-hapten IgE and IgG antibody responses by isologous anti-idiotypic antibodies against purified anti-carrier (ovalbumin) antibodies in BALB/c mice. J Immunol. 1981 Mar;126(3):1180–1184. [PubMed] [Google Scholar]
  3. Blaser K., Nakagawa T., de Weck A. L. Suppression of the benzylpenicilloyl- (BPO) specific IgE formation with isologous anti-idiotypic antibodies in BALB/c mice. J Immunol. 1980 Jul;125(1):24–30. [PubMed] [Google Scholar]
  4. Dohi Y., Nisonoff A. Suppression of idiotype and generation of suppressor T cells with idiotype-conjugated thymocytes. J Exp Med. 1979 Oct 1;150(4):909–918. doi: 10.1084/jem.150.4.909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EISEN H. N. EQUILIBRIUM DIALYSIS FOR MEASUREMENT OF ANTIBODY-HAPTEN AFFINITIES. Methods Med Res. 1964;10:106–114. [PubMed] [Google Scholar]
  6. Eichmann K., Falk I., Rajewsky K. Recognition of idiotypes in lymphocyte interactions. II. Antigen-independent cooperation between T and B lymphocytes that possess similar and complementary idiotypes. Eur J Immunol. 1978 Dec;8(12):853–857. doi: 10.1002/eji.1830081206. [DOI] [PubMed] [Google Scholar]
  7. Eichmann K. Idiotypic identity of antibodies to streptococcal carbohydrate in inbred mice. Eur J Immunol. 1972 Aug;2(4):301–307. doi: 10.1002/eji.1830020402. [DOI] [PubMed] [Google Scholar]
  8. Herzenberg L. A., Black S. J., Herzenberg L. A. Regulatory circuits and antibody responses. Eur J Immunol. 1980 Jan;10(1):1–11. doi: 10.1002/eji.1830100102. [DOI] [PubMed] [Google Scholar]
  9. Janeway C. A., Jr, Paul W. E. Hapten-specific augmentation of the anti-idiotype antibody response to hapten-myeloma protein conjugates in mice. Eur J Immunol. 1973 Jun;3(6):340–347. doi: 10.1002/eji.1830030605. [DOI] [PubMed] [Google Scholar]
  10. Kishimoto T., Ishizaka K. Regulation of antibody response in vitro. VI. Carrier-specific helper cells for IgG and IgE antibody response. J Immunol. 1973 Sep;111(3):720–732. [PubMed] [Google Scholar]
  11. Mitchison N. A. The carrier effect in the secondary response to hapten-protein conjugates. I. Measurement of the effect with transferred cells and objections to the local environment hypothesis. Eur J Immunol. 1971 Jan;1(1):10–17. doi: 10.1002/eji.1830010103. [DOI] [PubMed] [Google Scholar]
  12. Nakagawa T., Blaser K., de Weck A. L. Effect of epitope density on the induction of the IgE immune response in mice. Int Arch Allergy Appl Immunol. 1980;63(2):212–219. doi: 10.1159/000232629. [DOI] [PubMed] [Google Scholar]
  13. OVARY Z., BENACERRAF B. IMMUNOLOGICAL SPECIFICITY OF THE SECONDARY RESPONSE WITH DINITROPHENYLATED PROTEINS. Proc Soc Exp Biol Med. 1963 Oct;114:72–76. doi: 10.3181/00379727-114-28589. [DOI] [PubMed] [Google Scholar]
  14. Ovary Z., Caiazza S. S., Kojima S. PCA reactions with mouse antibodies in mice and rats. Int Arch Allergy Appl Immunol. 1975;48(1):16–21. doi: 10.1159/000231289. [DOI] [PubMed] [Google Scholar]
  15. Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol Rev. 1972 Jul;52(3):631–719. doi: 10.1152/physrev.1972.52.3.631. [DOI] [PubMed] [Google Scholar]
  16. Potter M., Leon M. A. Three IgA myeloma immunoglobulins from the BALB/ mouse: precipitation with pneumococcal C polysaccharide. Science. 1968 Oct 18;162(3851):369–371. doi: 10.1126/science.162.3851.369. [DOI] [PubMed] [Google Scholar]
  17. Rajewsky K., Schirrmacher V., Nase S., Jerne N. K. The requirement of more than one antigenic determinant for immunogenicity. J Exp Med. 1969 Jun 1;129(6):1131–1143. doi: 10.1084/jem.129.6.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sakato N., Eisen H. N. Antibodies to idiotypes of isologous immunoglobulins. J Exp Med. 1975 Jun 1;141(6):1411–1426. doi: 10.1084/jem.141.6.1411. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schneider C. H., de Weck A. L. Chemische Aspekte der Penicillin-Allergie: Die direkte Penicilloylierung von epsilon-Aminogruppen durch Penicilline bei pH 7,4. Helv Chim Acta. 1966 Jul 11;49(5):1695–1706. doi: 10.1002/hlca.19660490532. [DOI] [PubMed] [Google Scholar]
  20. Sirisinha S., Eisen H. N. Autoimmune-like antibodies to the ligand-binding sites of myeloma proteins. Proc Natl Acad Sci U S A. 1971 Dec;68(12):3130–3135. doi: 10.1073/pnas.68.12.3130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tada T., Takemori T., Okumura K., Nonaka M., Tokuhisa T. Two distinct types of helper T cells involved in the secondary antibody response: independent and synergistic effects of Ia- and Ia+ helper T cells. J Exp Med. 1978 Feb 1;147(2):446–458. doi: 10.1084/jem.147.2.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tung A. S., Ju S. T., Sato S., Nisonoff A. Production of large amounts of antibodies in individual mice. J Immunol. 1976 Mar;116(3):676–681. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES