Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1983 Apr;48(4):743–754.

Two distinct antigenic markers for rat thymus and T cells defined by monoclonal antibodies.

Y Ishii, A Matsuura, H Yuasa, H Narita, T Takami, K Kikuchi
PMCID: PMC1454075  PMID: 6339377

Abstract

Rat thymus and T-cell antigens were defined by using two distinct monoclonal antibodies (R-1-3B3 and R-1-12C5). R-1-3B3 antibody, when tested for its reactivity with rat lymphoid cells by immunofluorescence, labelled virtually all thymus and T cells but not B cells and bone marrow cells. The antigen defined by this R-1-3B3 antibody occurred more abundantly on medullary thymocytes and peripheral T cells than on cortical thymocytes. Immunochemical data showed that R-1-3B3 antibody recognized a single glycoprotein with a molecular weight of 67,000, which were able to interact with Lens culinaris haemagglutinin. R-1-12C5 antibody, on the other hand, reacted with all of thymus and T cells as well as with a subpopulation (approximately 20%) of bone marrow cells. In contrast to the antigen defined by R-1-3B3, that detected by R-1-12C5 antibody existed largely on cortical thymocytes and to a much lesser extent on medullary thymocytes and peripheral T cells. R-1-12C5 antibody detected a single glycoprotein with a 95,000 molecular weight, which could also interact with Lens culinaris haemagglutinin. Based on these data described above and since both antigens defined by R-1-3B3 and R-1-12C5 antibodies were absent from rat brain tissue, we concluded that they were distinct from brain-associated thymic antigens in rats including Thy-1 and W3/13 antigen systems.

Full text

PDF
745

Images in this article

749Figure 1
on p.749
750Figure 2
on p.750
751Figure 3
on p.751
751Figure 4
on p.751
751Figure 5
on p.751
752Figure 6
on p.752

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ades E. W., Zwerner R. K., Acton R. T., Balch C. M. Isolation and partial characterization of the human homologue of Thy-1. J Exp Med. 1980 Feb 1;151(2):400–406. doi: 10.1084/jem.151.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barclay A. N. The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593–600. [PMC free article] [PubMed] [Google Scholar]
  3. Boyse E. A., Miyazawa M., Aoki T., Old L. J. Ly-A and Ly-B: two systems of lymphocyte isoantigens in the mouse. Proc R Soc Lond B Biol Sci. 1968 Jun 11;170(1019):175–193. doi: 10.1098/rspb.1968.0032. [DOI] [PubMed] [Google Scholar]
  4. Crawford J. M., Goldschneider I. THY1 antigen and B lymphocyte differentiation in the rat. J Immunol. 1980 Feb;124(2):969–976. [PubMed] [Google Scholar]
  5. Cullen S. E., Schwartz B. D. An improved method for isolation of H-2 and Ia alloantigens with immunoprecipitation induced by protein A-bearing staphylococci. J Immunol. 1976 Jul;117(1):136–142. [PubMed] [Google Scholar]
  6. Dalchau R., Fabre J. W. Identification and unusual tissue distribution of the canine and human homologues of Thy-1 (theta). J Exp Med. 1979 Mar 1;149(3):576–591. doi: 10.1084/jem.149.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Durda P. J., Shapiro C., Gottlieb P. D. Partial molecular characterization of the Ly-1 alloantigen on mouse thymocytes. J Immunol. 1978 Jan;120(1):53–57. [PubMed] [Google Scholar]
  8. Goldschneider I., McGregor D. D. Anatomical distribution of T and B lymphocytes in the rat. Development of lymphocyte-specific antisera. J Exp Med. 1973 Dec 1;138(6):1443–1465. doi: 10.1084/jem.138.6.1443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Howard F. D., Ledbetter J. A., Wong J., Bieber C. P., Stinson E. B., Herzenberg L. A. A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E-rosette formation. J Immunol. 1981 Jun;126(6):2117–2122. [PubMed] [Google Scholar]
  10. Hunt S. V. The presence of Thy-1 on the surface of rat lymphoid stem cells and colony-forming units. Eur J Immunol. 1979 Nov;9(11):853–859. doi: 10.1002/eji.1830091105. [DOI] [PubMed] [Google Scholar]
  11. Ishii Y., Fujimoto J., Kon S., Ogasawara M., Koshiba H., Matsuura A., Kikuchi K. Immunochemical characterization of human TL-like (T48) and Ly 1-like (T72) glycoproteins using two-dimensional polyacrylamide gel electrophoresis. Clin Exp Immunol. 1981 Dec;46(3):530–540. [PMC free article] [PubMed] [Google Scholar]
  12. Ishii Y., Fujimoto J., Koshiba H., Kikuchi K. Isolation and partial characterization of a 72,000-dalton glycoprotein (Tgp72) on human thymus and T cells: possible relationship to mouse Ly-1 antigens. J Immunol. 1981 Jun;126(6):2171–2176. [PubMed] [Google Scholar]
  13. Ishii Y., Koshiba H., Yamaoka H., Kikuchi K. Rat T lymphocyte-specific antigens and their cross-reactivity with mouse T cells. J Immunol. 1976 Aug;117(2):497–503. [PubMed] [Google Scholar]
  14. Ishii Y., Ueno H., Kikuchi K. Ultrastructure of lymphoid cells with surface-bound immunoglobulin in rats. J Reticuloendothel Soc. 1974 Feb;15(2):155–162. [PubMed] [Google Scholar]
  15. Itakura K., Hutton J. J., Boyse E. A., Old L. J. Genetic linkage relationships of loci specifying differentiation alloantigens in the mouse. Transplantation. 1972 Mar;13(3):239–243. doi: 10.1097/00007890-197203000-00007. [DOI] [PubMed] [Google Scholar]
  16. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  17. Kumararatne D. S., Bazin H., MacLennan I. C. Marginal zones: the major B cell compartment of rat spleens. Eur J Immunol. 1981 Nov;11(11):858–864. doi: 10.1002/eji.1830111103. [DOI] [PubMed] [Google Scholar]
  18. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  19. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  20. Ledbetter J. A., Evans R. L., Lipinski M., Cunningham-Rundles C., Good R. A., Herzenberg L. A. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981 Feb 1;153(2):310–323. doi: 10.1084/jem.153.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  22. Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280–295. doi: 10.1084/jem.152.2.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Lemke H., Hammerling G. J., Hohmann C., Rajewsky K. Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature. 1978 Jan 19;271(5642):249–251. doi: 10.1038/271249a0. [DOI] [PubMed] [Google Scholar]
  24. McCabe R. P., Quaranta V., Frugis L., Ferrone S., Reisfeld R. A. A radioimmunometric antibody-binding assay for evaluation of xenoantisera to melanoma-associated antigens. J Natl Cancer Inst. 1979 Mar;62(3):455–463. doi: 10.1093/jnci/62.3.455. [DOI] [PubMed] [Google Scholar]
  25. McLean I. W., Nakane P. K. Periodate-lysine-paraformaldehyde fixative. A new fixation for immunoelectron microscopy. J Histochem Cytochem. 1974 Dec;22(12):1077–1083. doi: 10.1177/22.12.1077. [DOI] [PubMed] [Google Scholar]
  26. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Reinherz E. L., Kung P. C., Goldstein G., Levey R. H., Schlossman S. F. Discrete stages of human intrathymic differentiation: analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1588–1592. doi: 10.1073/pnas.77.3.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Standring R., McMaster W. R., Sunderland C. A., Williams A. F. The predominant heavily glycosylated glycoproteins at the surface of rat lymphoid cells are differentiation antigens. Eur J Immunol. 1978 Dec;8(12):832–839. doi: 10.1002/eji.1830081203. [DOI] [PubMed] [Google Scholar]
  29. Uede T., Ishii Y., Matsuura A., Shimogawara I., Kikuchi K. Immunohistochemical study of lymphocytes in rat pineal gland: selective accumulation of T lymphocytes. Anat Rec. 1981 Feb;199(2):239–247. doi: 10.1002/ar.1091990208. [DOI] [PubMed] [Google Scholar]
  30. Verbi W., Greaves M. F., Schneider C., Koubek K., Janossy G., Stein H., Kung P., Goldstein G. Monoclonal antibodies OKT 11 and OKT 11A have pan-T reactivity and block sheep erythrocyte "receptors". Eur J Immunol. 1982 Jan;12(1):81–86. doi: 10.1002/eji.1830120115. [DOI] [PubMed] [Google Scholar]
  31. Williams A. F., Galfrè G., Milstein C. Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes. Cell. 1977 Nov;12(3):663–673. doi: 10.1016/0092-8674(77)90266-5. [DOI] [PubMed] [Google Scholar]
  32. Williams A. F. Many cells in rat bone marrow have cell-surface Thy-1 antigen. Eur J Immunol. 1976 Jul;6(7):526–528. doi: 10.1002/eji.1830060716. [DOI] [PubMed] [Google Scholar]
  33. Yamanaka N., Ishii Y., Koshiba H., Kikuchi K. Immunohistological detection of B lymphocytes in the rat lymphoid organs by using anti-rat B lymphocyte serum. Tohoku J Exp Med. 1981 Oct;135(2):187–196. doi: 10.1620/tjem.135.187. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES