Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1983 Jul;49(3):511–518.

The successful use of asialylated IgG as an immunogen and arthritogen in the rabbit.

G Galloway, A Y Leung, I M Hunneyball, D R Stanworth
PMCID: PMC1454282  PMID: 6862524

Abstract

Joint lesions, closely resembling the main features of those seen in rheumatoid patients, were produced by intra-articular injections of asialylated homologous IgG into presensitized rabbits. The inflammatory changes were characterized by areas of extremely dense chronic inflammatory cell infiltration, where the lymphocytes were often aggregated into lymphoid follicles. There were also signs of involvement of the contralateral, saline-injected knee. Formation of an experimental rheumatoid factor-like antibody, detected by its ability to agglutinate sheep erythrocytes sensitized with baboon IgG, was also demonstrated. In addition, the rabbits developed other manifestations associated with rheumatoid arthritis, namely increases in erythrocyte sedimentation rate, serum haptoglobin concentration and joint size.

Full text

PDF
514

Images in this article

515Figure 5
on p.515
516Figure 6
on p.516

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Consden R., Doble A., Glynn L. E., Nind A. P. Production of a chronic arthritis with ovalbumin. Its retention in the rabbit knee joint. Ann Rheum Dis. 1971 May;30(3):307–315. doi: 10.1136/ard.30.3.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DUMONDE D. C., GLYNN L. E. The production of arthritis in rabbits by an immunological reaction to fibrin. Br J Exp Pathol. 1962 Aug;43:373–383. [PMC free article] [PubMed] [Google Scholar]
  3. Dodon M. D., Quash G. A. The antigenicity of asialylated IgG: its relationship to rheumatoid factor. Immunology. 1981 Mar;42(3):401–408. [PMC free article] [PubMed] [Google Scholar]
  4. Glynn L. G. Recent concepts on the pathogenesis of rheumatoid arthritis. Ric Clin Lab. 1977 Oct-Dec;7(4):299–311. doi: 10.1007/BF02886644. [DOI] [PubMed] [Google Scholar]
  5. Henney C. S., Stanworth D. R. The reactivity of rheumatoid factor with human gamma G globulin. Immunology. 1965 Aug;9(2):139–150. [PMC free article] [PubMed] [Google Scholar]
  6. Hunneyball I. M., Stanworth D. R. The effects of chemical modification on the antigenicity of human and rabbit immunoglobulin G. Immunology. 1976 Jun;30(6):881–894. [PMC free article] [PubMed] [Google Scholar]
  7. Jasin H. E., Cooke T. D. The inflammatory role of immune complexes trapped in joint collagenous tissues. Clin Exp Immunol. 1978 Sep;33(3):416–424. [PMC free article] [PubMed] [Google Scholar]
  8. MILGROM F., WITEBSKY E. Studies on the rheumatoid and related serum factors. I. Autoimmunization of rabbits with gamma globulin. JAMA. 1960 Sep 3;174:56–63. doi: 10.1001/jama.1960.63030010013013. [DOI] [PubMed] [Google Scholar]
  9. Murayama J. I., Tomita M., Tsuji A., Hamada A. Fluorimetric assay of sialic acids. Anal Biochem. 1976 Jun;73(2):535–538. doi: 10.1016/0003-2697(76)90204-9. [DOI] [PubMed] [Google Scholar]
  10. Stewart G. A., Hunneyball I. M., Stanworth D. R. The use of baboon IgG-sensitized sheep erythrocytes as an alternative indicator system in the study of the interaction of rheumatoid factor with human IgG. Immunochemistry. 1975 Aug;12(8):657–662. doi: 10.1016/0019-2791(75)90211-6. [DOI] [PubMed] [Google Scholar]
  11. Vaughan J. H. Dunlop-Dottridge lecture. Rehumatoid arthritis, rheumatoid factor and the Epstein-Barr virus. J Rheumatol. 1979 Jul-Aug;6(4):381–388. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES