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. 1983 Dec;50(4):605–612.

Enhancement of immunogenicity by incorporation of lipid A into liposomal model membranes and its application to membrane-associated antigens

H Tamauchi, T Tadakuma, T Yasuda, T Tsumita, K Saito
PMCID: PMC1454377  PMID: 6360851

Abstract

Incorporation of lipid A into liposomes markedly enhances the plaque-forming cell (PFC responses against model membranes that are sensitized with synthetic amphipathic antigens 2,4-dinitrophenyl-6-N-aminocaproyldipalmytoylphosphatidylethanolamine (DNP-Cap-DPPE) and fluorescein thiocarbamyldipalmytoylphosphatidylethanolamine (F1-DPPE). The enhancing effect was pronounced only when lipid A and haptenic determinants were presented on the same liposome.

Lipid A was incorporated into heterologous erythrocytes and syngeneic tumour cells. Significant and selective increase of in-vitro PFC responses was observed against heterologous erythrocytes, and immunization of C57BL/6 mice with lipid A treated X-irradiated tumour cells (EL-4) markedly prolonged the survival of mice after challenge with homologous viable tumour cells. These data suggest that passively incorporated lipid A will be an effective method for selectively augmenting antibody formation against membrane-associated antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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