Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1984 Jan;51(1):17–25.

Monoclonal antibodies reactive with idiotypic and variable-region specific determinants on human immunoglobulins.

J L Greenstein, A Solomon, G N Abraham
PMCID: PMC1454402  PMID: 6197358

Abstract

Monoclonal antibody JG-B1, specific for the human VKIIIb sub-subgroup of L chains, and JG-B4 specific for an idiotypic determinant on Glo, a monoclonal human IgM-VKIIIb anti-IgG, were produced and characterized. The VKIIIb determinant was detected on L chains alone and intact immunoglobulins with VKIIIb L chains. However, the idiotypic determinant was expressed only on IgM-Glo and required association of H and L chains. Binding of the immunogen Glo, to its antigen-IgG partially inhibited anti-idiotype and anti-VKIIIb binding. Cross-inhibition experiments demonstrated that intact pentameric IgM-Glo expressed one-half the number of idiotypic sites as VKIIIb determinants. However, Glo half-molecules expressed equal numbers of idiotypic and VKIIIb determinants. This is the first described monoclonal antibody produced by hybridoma technology which recognizes an antigenic determinant specific for a single variable region in intact immunoglobulin.

Full text

PDF
25

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham G. N., Brown P., Johnston S. L., Nellis L., Marks S., Welch E. H. Human triclonal anti-IgG gammopathy. III. Determination of the clonal persistence of the IgM autoantibody for a four and one-half year period. Immunology. 1978 Sep;35(3):447–453. [PMC free article] [PubMed] [Google Scholar]
  2. Abraham G. N., Clark R. A., Vaughan J. H. Characterization of an IgA rheumatoid factor: finding properties and reactivity with the subclasses of human G globulin. Immunochemistry. 1972 Mar;9(3):301–315. doi: 10.1016/0019-2791(72)90094-8. [DOI] [PubMed] [Google Scholar]
  3. Agnello V., Arbetter A., Ibanez de Kasep G., Powell R., Tan E. M., Joslin F. Evidence for a subset of rheumatoid factors that cross-react with DNA-histone and have a distinct cross-idiotype. J Exp Med. 1980 Jun 1;151(6):1514–1527. doi: 10.1084/jem.151.6.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Capra J. D., Kehoe J. M. Hypervariable regions, idiotypy, and the antibody-combining site. Adv Immunol. 1975;20:1–40. doi: 10.1016/s0065-2776(08)60205-9. [DOI] [PubMed] [Google Scholar]
  5. Capra J. D., Kehoe J. M. Structure of antibodies with shared idiotypy: the complete sequence of the heavy chain variable regions of two immunoglobulin M anti-gamma globulins. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4032–4036. doi: 10.1073/pnas.71.10.4032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Capra J. D., Klapper D. G. Complete amino acid sequence of the variable domains of two human IgM anti-gamma globulins (Lay/Pom) with shared idiotypic specificities. Scand J Immunol. 1976;5(6-7):677–684. doi: 10.1111/j.1365-3083.1976.tb03017.x. [DOI] [PubMed] [Google Scholar]
  7. Chiller J. M., Weigle W. O. Cellular events during induction of immunologic unresponsiveness in adult mice. J Immunol. 1971 Jun;106(6):1647–1653. [PubMed] [Google Scholar]
  8. Doran D. M., Spar I. L. Oxidative iodine monochloride iodination technique. J Immunol Methods. 1980;39(1-2):155–163. doi: 10.1016/0022-1759(80)90304-x. [DOI] [PubMed] [Google Scholar]
  9. FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
  10. Feizi T., Kunkel H. G., Roelcke D. Cross idiotypic specificity among cold agglutinins in relation to combining activity for blood group-related antigens. Clin Exp Immunol. 1974 Oct;18(2):283–293. [PMC free article] [PubMed] [Google Scholar]
  11. Franklin E. C., Frangione B. The molecular defect in a protein (CRA) found in gamma-1 heavy chain disease, and its genetic implications. Proc Natl Acad Sci U S A. 1971 Jan;68(1):187–191. doi: 10.1073/pnas.68.1.187. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Førre O., Dobloug J. H., Michaelsen T. E., Natvig J. B. Evidence of similar idiotypic determinants on different rheumatoid factor populations. Scand J Immunol. 1979 Mar;9(3):281–289. doi: 10.1111/j.1365-3083.1979.tb02732.x. [DOI] [PubMed] [Google Scholar]
  13. Greenstein J. L., Lord E. M., Horan P., Kappler J. W., Marrack P. Functional subsets of B cells defined by quantitative differences in surface I-A. J Immunol. 1981 Jun;126(6):2419–2423. [PubMed] [Google Scholar]
  14. Johnston S. L., Abraham G. N. Studies of human anti-IgM anti-IgG cryoglobulins. I. Patterns of reactivity with autologous and isologous human IgG and its subunits. Immunology. 1979 Apr;36(4):671–683. [PMC free article] [PubMed] [Google Scholar]
  15. Johnston S. L., Abraham G. N., Welch E. H. Structural studies of monoclonal human cryoprecipitable immunoglobulins. Biochem Biophys Res Commun. 1975 Sep 16;66(2):842–847. doi: 10.1016/0006-291x(75)90586-0. [DOI] [PubMed] [Google Scholar]
  16. Kubagawa H., Mayumi M., Kearney J. F., Cooper M. D. Immunoglobulin VH determinants defined by monoclonal antibodies. J Exp Med. 1982 Oct 1;156(4):1010–1024. doi: 10.1084/jem.156.4.1010. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kunkel H. G., Agnello V., Joslin F. G., Winchester R. J., Capra J. D. Cross-idiotypic specificity among monoclonal IgM proteins with anti- -globulin activity. J Exp Med. 1973 Feb 1;137(2):331–342. doi: 10.1084/jem.137.2.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kunkel H. G., Joslin F., Hurley J. Blocking of certain antigenic sites in the F(ab) region by combination of univalent fragments of Rh antibodies with red cell antigens. J Immunol. 1976 Jun;116(6):1532–1535. [PubMed] [Google Scholar]
  19. Kunkel H. G., Winchester R. J., Joslin F. G., Capra J. D. Similarities in the light chains of anti-gamma-globulins showing cross-idiotypic specificities. J Exp Med. 1974 Jan 1;139(1):128–136. doi: 10.1084/jem.139.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  21. McLaughlin C. L., Solomon A. Bence-Jones proteins and light chains of immunoglobulins. VII. Localization of antigenic sites responsible for immunochemical heterogeneity of kappa chains. J Biol Chem. 1972 Aug 25;247(16):5017–5025. [PubMed] [Google Scholar]
  22. Solomon A., McLaughlin C. L. Bence Jones proteins and light chains of immunoglobulins. II. Immunochemical differentiation and classification of kappa-chains. J Exp Med. 1969 Dec 1;130(6):1295–1311. doi: 10.1084/jem.130.6.1295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wang A. C., Wells J. V., Fudenberg H. H. Chemical analyses of cryoglobulins. Immunochemistry. 1974 Jul;11(7):341–345. doi: 10.1016/0019-2791(74)90186-4. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES