Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1984 Aug;52(4):697–702.

Influence of subclass-specific anti-idiotypic antibodies on the kinetics of the immune response to BCG.

H Daus, H J Hammer, K Rajki, H Mauch
PMCID: PMC1454648  PMID: 6611300

Abstract

The role of anti-idiotypic subclass-specific antibodies was analysed in the regulation of the immune response to BCG in the guinea-pig. The idiotypes to BCG were separated into subclasses and anti-idiotypes were carried out by immunizing with the purified IgG1 and IgG2 anti-idiotypes. The in vivo T cell response was recorded by tuberculin skin testing, and the in vitro response by lymphocyte stimulation testing with tuberculin. A suppressive effect was detected in cases where the animals were preimmunized with anti-idiotypic IgG2 against anti-BCG IgG1. In the B cell response, the anti-BCG IgG1 and IgG2 subclasses were also quantified by a solid-phase radioimmunoassay. There were 10 times more IgG2 antibodies than IgG1 against BCG in the guinea-pig, and this major idiotypic subclass was suppressed by the IgG2 anti-idiotype raised against anti-BCG IgG2. The minor component anti-BCG IgG1 was slightly stimulated by both IgG2 anti-idiotypes.

Full text

PDF
700

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Eichmann K. Idiotype expression and the inheritance of mouse antibody clones. J Exp Med. 1973 Mar 1;137(3):603–621. doi: 10.1084/jem.137.3.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Eichmann K. Idiotype suppression. I. Influence of the dose and of the effector functions of anti-idiotypic antibody on the production of an idiotype. Eur J Immunol. 1974 Apr;4(4):296–302. doi: 10.1002/eji.1830040413. [DOI] [PubMed] [Google Scholar]
  3. Eichmann K. Idiotype suppression. II. Amplification of a suppressor T cell with anti-idiotypic activity. Eur J Immunol. 1975 Aug;5(8):511–517. doi: 10.1002/eji.1830050802. [DOI] [PubMed] [Google Scholar]
  4. Goidl E. A., Schrater A. F., Thorbecke G. H., Siskind G. W. Production of auto-anti-idiotypic antibody during the normal immune response. IV. Studies of the primary and secondary responses to thymus-dependent and -independent antigens. Eur J Immunol. 1980 Nov;10(11):810–814. doi: 10.1002/eji.1830101103. [DOI] [PubMed] [Google Scholar]
  5. Hetzelberger D., Eichmann K. Idiotype suppression. III. Induction of unresponsiveness to sensitization with anti-idiotypic antibody: identification of the cell types tolerized in high zone and in low zone, suppressor cell-mediated, idiotype suppression. Eur J Immunol. 1978 Dec;8(12):839–846. doi: 10.1002/eji.1830081204. [DOI] [PubMed] [Google Scholar]
  6. Hjelm H., Hjelm K., Sjöquist J. Protein A from Staphylococcus aureus. Its isolation by affinity chromatography and its use as an immunosorbent for isolation of immunoglobulins. FEBS Lett. 1972 Nov 15;28(1):73–76. doi: 10.1016/0014-5793(72)80680-x. [DOI] [PubMed] [Google Scholar]
  7. Jerne N. K. Towards a network theory of the immune system. Ann Immunol (Paris) 1974 Jan;125C(1-2):373–389. [PubMed] [Google Scholar]
  8. Kato K., Yamamoto K. Suppression of BCG cell wall-induced delayed-type hypersensitivity by BCG pre-treatment. II. Induction of suppressor T cells by heat-killed BCG injection. Immunology. 1982 Apr;45(4):655–661. [PMC free article] [PubMed] [Google Scholar]
  9. Kümel G., Daus H., Mauch H. Improved method for the cyanogen bromide activation of agarose beads. J Chromatogr. 1979 Apr 21;172:221–226. doi: 10.1016/s0021-9673(00)90957-9. [DOI] [PubMed] [Google Scholar]
  10. Mauch H., Brehmer W. Mycobacterial antibodies after tuberculin testing, BCG- vaccination, BCG-immunotherapy and against cross-reacting antigens in a solid-phase radioimmunoassay. Zentralbl Bakteriol Mikrobiol Hyg A. 1982 Mar;251(3):380–388. [PubMed] [Google Scholar]
  11. Mauch H., Kümel G. A solid-phase radioimmunoassay to detect anti-tuberculin antibodies in the guinea pig. Int Arch Allergy Appl Immunol. 1979;58(1):82–89. doi: 10.1159/000232176. [DOI] [PubMed] [Google Scholar]
  12. Oliveira B., Osler A. G., Siraganian R. P., Sandberg A. L. The biologic activities of guinea pig antibodies. I. Separation of gamma1 and gamma2 immunoglobulins and their participation in allergic reactions of the immediate type. J Immunol. 1970 Feb;104(2):320–328. [PubMed] [Google Scholar]
  13. Schrier D. J., Sternick J. L., Allen E. M., Moore V. L. Immunogenetics of BCG-induced anergy in mice: control by genes linked to the Igh complex. J Immunol. 1982 Mar;128(3):1466–1469. [PubMed] [Google Scholar]
  14. Stewart-Tull D. E., Arbuthnott J. P., Freer J. H. Some properties of IgG1 and IgG2 globulins from normal and adjuvant stimulated guinea-pigs. Immunochemistry. 1975 Dec;12(12):941–947. doi: 10.1016/0019-2791(75)90257-8. [DOI] [PubMed] [Google Scholar]
  15. Sy M. S., Bach B. A., Brown A., Nisonoff A., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. II. Induction of suppressor T cells with idiotype-coupled syngeneic spleen cells. J Exp Med. 1979 Nov 1;150(5):1229–1240. doi: 10.1084/jem.150.5.1229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sy M. S., Bach B. A., Dohi Y., Nisonoff A., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. I. Induction of suppressor T cells with anti-idiotypic antibodies. J Exp Med. 1979 Nov 1;150(5):1216–1228. doi: 10.1084/jem.150.5.1216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sy M. S., Dietz M. H., Germain R. N., Benacerraf B., Greene M. I. Antigen- and receptor-driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induce second-order suppressor T cells which express anti-idiotypic receptors. J Exp Med. 1980 May 1;151(5):1183–1195. doi: 10.1084/jem.151.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Waldron J. A., Jr, Horn R. G., Rosenthal A. S. Antigen-induced proliferation of guinea pig lymphocytes in vitro: obligatory role of macrophages in the recognition of antigen by immune T-lymphocytes. J Immunol. 1973 Jul;111(1):58–64. [PubMed] [Google Scholar]
  19. Yamamoto H., Nonaka M., Katz D. H. Suppression of hapten-specific delayed-type hypersensitivity responses in mice by idiotype-specific suppressor T cells after administration of anti-idiotypic antibodies. J Exp Med. 1979 Oct 1;150(4):818–829. doi: 10.1084/jem.150.4.818. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES