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. 1984 Nov;53(3):481–490.

The relationship between secreted and cell surface antigen-binding molecules synthesized by T cells. I. Function-related isotypic determinants on T cells defined by antisera to antigen-specific helper and suppressor factors.

E J Culbert, S Kontiainen, M Feldmann
PMCID: PMC1454920  PMID: 6208146

Abstract

Rabbit antisera have been used to define 'constant region' markers which distinguish between mouse T cell-derived helper and suppressor factors, regardless of their antigen-specificity or strain of origin. These antisera have also been shown to bind to functional T-cell lines. After several absorption steps, rabbit anti-helper factor serum bound specifically to mouse helper cell lines, whereas rabbit anti-suppressor factor serum bound specifically to suppressor cell lines. Neither antiserum bound to cytotoxic T cell lines. The 'isotypic' determinants defined by these antisera were demonstrated to be present on distinct subpopulations of non-transformed T cell populations, such as splenic T cells, cortisone-resistant thymocytes and Con A blasts, but were not found on Thy-1- spleen cells, bone marrow, brain, heart, liver, kidney or heart tissue. The antisera did not stain significant numbers of normal thymocytes, and so expression was restricted to mature T cells. T cells reactive with rabbit anti-helper factor serum were found in the Lyt 2- population of cortisone-resistant thymocytes, and constituted a major subpopulation of in vitro induced helper cells, while rabbit anti-suppressor factor serum stained cells found in the Lyt 2+ population of cortisone-resistant thymocytes, as well as the majority of in vitro induced suppressor cells. Thus, these antisera are potentially of great value in the definition and isolation of functionally-distinct T cell subpopulations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ceredig R., Glasebrook A. L., MacDonald H. R. Phenotypic and functional properties of murine thymocytes. I. Precursors of cytolytic T lymphocytes and interleukin 2-producing cells are all contained within a subpopulation of "mature" thymocytes as analyzed by monoclonal antibodies and flow microfluorometry. J Exp Med. 1982 Feb 1;155(2):358–379. doi: 10.1084/jem.155.2.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Culbert E. J., Kontiainen S., Douglas L. M., Feldmann M. Definition of function-related isotypic markers on T cells. Curr Top Microbiol Immunol. 1982;100:19–32. doi: 10.1007/978-3-642-68586-6_3. [DOI] [PubMed] [Google Scholar]
  5. Feldmann M., Beverley P. C., Dunkley M., Kontiainen S. Different Ly antigen phenotypes of in vitro induced helper and suppressor cells. Nature. 1975 Dec 18;258(5536):614–616. doi: 10.1038/258614a0. [DOI] [PubMed] [Google Scholar]
  6. Feldmann M., Cecka J. M., James R., Todd I., Kontiainen S. Antibody production to antigen-specific factors. Contemp Top Immunobiol. 1980;11:275–289. doi: 10.1007/978-1-4684-3701-0_9. [DOI] [PubMed] [Google Scholar]
  7. Haskins K., Kubo R., White J., Pigeon M., Kappler J., Marrack P. The major histocompatibility complex-restricted antigen receptor on T cells. I. Isolation with a monoclonal antibody. J Exp Med. 1983 Apr 1;157(4):1149–1169. doi: 10.1084/jem.157.4.1149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Howie S., Feldman M. In vitro studies on H-2-linked unresponsiveness to synthetic polypeptides. III. Production of an antigen-specific T helper cell factor to (T,G)-A--L. Eur J Immunol. 1977 Jul;7(7):417–421. doi: 10.1002/eji.1830070703. [DOI] [PubMed] [Google Scholar]
  9. Kontiainen S., Feldmann M. Suppressor cell induction in vitro. I. Kinetics of induction of antigen-specific suppressor cells. Eur J Immunol. 1976 Apr;6(4):296–301. doi: 10.1002/eji.1830060412. [DOI] [PubMed] [Google Scholar]
  10. Lai P. K., Waterfield J. D., Gascoigne N. R., Sharrock C. E., Mitchison N. A. T-cell responses to minor histocompatibility antigens. Immunology. 1982 Oct;47(2):371–381. [PMC free article] [PubMed] [Google Scholar]
  11. Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280–295. doi: 10.1084/jem.152.2.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Okumura K., Herzenberg L. A., Murphy D. B., McDevitt H. O., Herzenberg L. A. Selective expression of H-2 (i-region) loci controlling determinants on helper and suppressor T lymphocytes. J Exp Med. 1976 Sep 1;144(3):685–698. doi: 10.1084/jem.144.3.685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ricciardi-Castagnoli P., Doria G., Adorini L. Production of antigen-specific suppressive T cell factor by radiation leukemia virus-transformed suppressor T cells. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3804–3808. doi: 10.1073/pnas.78.6.3804. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rogers J., Early P., Carter C., Calame K., Bond M., Hood L., Wall R. Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain. Cell. 1980 Jun;20(2):303–312. doi: 10.1016/0092-8674(80)90616-9. [DOI] [PubMed] [Google Scholar]

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