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. 1970 Apr;18(4):585–594.

Fractionation of immunocompetent spleen cells by albumin density gradient centrifugation

Göran Möller, Klaus Hiesche
PMCID: PMC1455500  PMID: 4393021

Abstract

Fractionation of spleen cells was performed on albumin density gradients. Larger cells localized in the low density fractions. 19S and 7S antibody producing cells against SRBC and Esch. coli endotoxin were found to be enriched in low density fractions; high density fractions being depleted of such cells. Antigen-sensitive cells for humoral antibody synthesis were studied in transfer system and were found to be enriched in the high density fractions. Cells initiating graft-versus-host reactions were also enriched in these fractions. The results suggest that the antibody-producing cells are physically distinct from the antigen-sensitive cells initiating humoral antibody synthesis and cell-mediated immunity as well.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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