Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1970 Mar;18(3):417–429.

Immunoglobulin determinants on the lymphocytes of normal rabbits

I. Demonstration by the mixed antiglobulin reaction of determinants recognized by anti-γ, anti-μ, anti-Fab and anti-allotype sera, anti-As4 and anti-As6

R R A Coombs, B W Gurner, C A Janeway, Anne B Wilson, P G H Gell, A S Kelus
PMCID: PMC1455568  PMID: 4192262

Abstract

Immunoglobulin determinants can be shown on the membrane of circulating lymphocytes of the rabbit by the mixed antiglobulin reaction. An enumeration was made of lymphocytes reacting specifically with anti-γ, anti-μ, anti-Fab and anti-L chain sera. The L chain allotypic determinants As4 and As6 were also shown and reacting cells enumerated. There was a marked dose-dependence between the concentration of the antiglobulin reagent used to treat the lymphocyte suspension and the number of lymphocytes reacting to form rosettes.

The experiments reported at this stage bear no direct evidence on the question as to whether the μ- and γ-determinants, or more than one L chain allotypic determinant can exist on the one cell.

Of three rabbits with 26, 47, and 61 per cent IgG-reacting lymphocytes in the circulation, only one had 1 per cent reacting cells in the thymus while the other two had less than 1 per cent. No μ-reacting cells were detectable in the thymuses of the two rabbits tested.

Full text

PDF
423

Images in this article

421FIG. 2
on p.421

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHALMERS D. G., COOMBS R. R., GURNER B. W., DAUSSET J. The mixed antiglobulin reaction in the detection of human isoantibodies against leucocytes, platelets and HeLa cells. Br J Haematol. 1959 Jul;5:225–231. doi: 10.1111/j.1365-2141.1959.tb04030.x. [DOI] [PubMed] [Google Scholar]
  2. CHAPLIN H., COHEN S., PRESS E. M. PREPARATION AND PROPERTIES OF THE PEPTIDE CHAINS OF NORMAL HUMAN 19 S GAMMA-GLOBULIN (IGM). Biochem J. 1965 Apr;95:256–261. doi: 10.1042/bj0950256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUBISKI S., DUDZIAK Z., SKALBA D. Serum groups in rabbits. Immunology. 1959 Jan;2(1):84–92. [PMC free article] [PubMed] [Google Scholar]
  4. Pernis B., Chiappino G., Kelus A. S., Gell P. G. Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J Exp Med. 1965 Nov 1;122(5):853–876. doi: 10.1084/jem.122.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sell S., Gell P. G. Studies on rabbit lymphocytes in vitro. IV. Blast transformation of the lymphocytes from newborn rabbits induced by antiallotype serum to a paternal IgG allotype not present in the serum of the lymphocyte donors. J Exp Med. 1965 Nov 1;122(5):923–928. doi: 10.1084/jem.122.5.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sell S. Studies on rabbit lymphocytes in vitro. V. The induction of blast transformation with sheep antisera to rabbit IgG subunits. J Exp Med. 1967 Feb 1;125(2):289–301. doi: 10.1084/jem.125.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sell S. Studies on rabbit lymphocytes in vitro. VI. The induction of blast transformation with sheep antisera to rabbit IgA and IgM. J Exp Med. 1967 Mar 1;125(3):393–400. doi: 10.1084/jem.125.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES