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. 1970 May;18(5):681–691.

The circulatory behaviour of normal and enzyme altered thymocytes in rats

S N Berney, B M Gesner
PMCID: PMC1455584  PMID: 5420724

Abstract

The circulation of rat thymocytes and factors which may control their pathway were studied. Evidence was obtained that intravenously infused thymocytes migrate from blood to spleen and lymph nodes in a manner similar to lymphocytes, though in lesser amounts. Thymocytes, incubated with neuraminidase prior to intravenous infusion, exhibit markedly altered cell migration. Enzyme-treated thymocytes concentrate in the liver with little accumulation in the spleen. Subsequently, however, small numbers of cells migrate to lymph nodes. Trypsin treatment does not alter the migratory pathway of thymocytes. The results suggest that thymocytes migrate from blood to peripheral lymphoid tissue and that sugar constituents may determine the circulatory pathway.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. GESNER B. M., GINSBURG V. EFFECT OF GLYCOSIDASES ON THE FATE OF TRANSFUSED LYMPHOCYTES. Proc Natl Acad Sci U S A. 1964 Sep;52:750–755. doi: 10.1073/pnas.52.3.750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  3. GOWANS J. L. The effect of the continuous re-infusion of lymph and lymphocytes on the output of lymphocytes from the thoracic duct of unanaesthetized rats. Br J Exp Pathol. 1957 Feb;38(1):67–78. [PMC free article] [PubMed] [Google Scholar]
  4. GOWANS J. L. The fate of parental strain small lymphocytes in F1 hybrid rats. Ann N Y Acad Sci. 1962 Oct 24;99:432–455. doi: 10.1111/j.1749-6632.1962.tb45326.x. [DOI] [PubMed] [Google Scholar]
  5. GOWANS J. L. The recirculation of lymphocytes from blood to lymph in the rat. J Physiol. 1959 Apr 23;146(1):54–69. doi: 10.1113/jphysiol.1959.sp006177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldschneider I., McGregor D. D. Migration of lymphocytes and thymocytes in the rat. I. The route of migration from blood to spleen and lymph nodes. J Exp Med. 1968 Jan 1;127(1):155–168. doi: 10.1084/jem.127.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldschneider I., McGregor D. D. Migration of lymphocytes and thymocytes in the rat. II. Circulation of lymphocytes and thymocytes from blood to lymph. Lab Invest. 1968 Apr;18(4):397–406. [PubMed] [Google Scholar]
  8. HARRIS J. E., FORD C. E. CELLULAR TRAFFIC OF THE THYMUS: EXPERIMENTS WITH CHROMOSOME MARKERS. EVIDENCE THAT THE THYMUS PLAYS AN INSTRUCTIONAL PART. Nature. 1964 Feb 29;201:884–885. doi: 10.1038/201884a0. [DOI] [PubMed] [Google Scholar]
  9. MARCHESI V. T., GOWANS J. L. THE MIGRATION OF LYMPHOCYTES THROUGH THE ENDOTHELIUM OF VENULES IN LYMPH NODES: AN ELECTRON MICROSCOPE STUDY. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:283–290. doi: 10.1098/rspb.1964.0002. [DOI] [PubMed] [Google Scholar]
  10. MARCHESI V. T. The site of leucocyte emigration during inflammation. Q J Exp Physiol Cogn Med Sci. 1961 Apr;46:115–118. doi: 10.1113/expphysiol.1961.sp001522. [DOI] [PubMed] [Google Scholar]
  11. MILLER J. F. THE THYMUS AND THE DEVELOPMENT OF IMMUNOLOGIC RESPONSIVENESS. Science. 1964 Jun 26;144(3626):1544–1551. doi: 10.1126/science.144.3626.1544. [DOI] [PubMed] [Google Scholar]
  12. MURRAY R. G., WOODS P. A. STUDIES ON THE FATE OF LYMPHOCYTES. 3. THE MIGRATION AND METAMORPHOSIS OF IN SITU LABELED THYMIC LYMPHOCYTES. Anat Rec. 1964 Oct;150:113–128. doi: 10.1002/ar.1091500203. [DOI] [PubMed] [Google Scholar]
  13. NOSSAL G. J. STUDIES ON THE RATE OF SEEDING OF LYMPHOCYTES FROM THE INTACT GUINEA PIG THYMUS. Ann N Y Acad Sci. 1964 Nov 30;120:171–181. doi: 10.1111/j.1749-6632.1964.tb34715.x. [DOI] [PubMed] [Google Scholar]
  14. SAINTE-MARIE G., LEBLOND C. P. Origin and fate of cells in the medulla of rat thymus. Proc Soc Exp Biol Med. 1958 Aug-Sep;98(4):909–915. doi: 10.3181/00379727-98-24226. [DOI] [PubMed] [Google Scholar]
  15. SHORTER R. G., BOLLMAN J. L. Experimental transfusion of lymphocytes. Am J Physiol. 1960 May;198:1014–1018. doi: 10.1152/ajplegacy.1960.198.5.1014. [DOI] [PubMed] [Google Scholar]
  16. Weissman I. L. Thymus cell migration. J Exp Med. 1967 Aug 1;126(2):291–304. doi: 10.1084/jem.126.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Woodruff J. J., Gesner B. M. The effect of neuraminidase on the fate of transfused lymphocytes. J Exp Med. 1969 Mar 1;129(3):551–567. doi: 10.1084/jem.129.3.551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Woodruff J., Gesner B. M. Lymphocytes: circulation altered by trypsin. Science. 1968 Jul 12;161(3837):176–178. doi: 10.1126/science.161.3837.176. [DOI] [PubMed] [Google Scholar]

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