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. 1971 Mar;20(3):299–306.

A C1-fixation method for the measurement of chicken anti-viral antibody

R L Stolfi, Ruth A Fugmann, J J Jensen, M M Sigel
PMCID: PMC1455823  PMID: 4323923

Abstract

Because of the failure of chicken antibody to activate the first component (C1) of guinea-pig complement a haemolytic system for the measurement of chicken C1 was developed. Chicken antisera to influenza and Rous sarcoma viruses were then assayed by the measurement of the fixation of chicken C1. Antibody titres obtained with this C1-fixation assay correlated well with those obtained by other methods.

The haemolytic system consisted of sheep erythrocytes sensitized with chicken antibody, partially purified preparations of chicken C1, and a guinea-pig serum reagent free of C1, but containing the remaining eight components of complement. Measurement of the residual chicken C1 by this method was profoundly influenced by ionic strength during both the fixation and the lytic phases, and by the order of addition of the reagents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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