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. 1969 Oct;17(4):535–550.

Effect of RES `blockade' on antibody formation

II. Cytokinetics of the secondary haemolysin response and suppressed immunological `memory' in mice treated with carbon particles

T Y Sabet, H Friedman
PMCID: PMC1455850  PMID: 5352362

Abstract

Suppression of the primary immune response by treatment of mice with carbon 1 day before initial immunization markedly interfered with development of immunological `memory', since such mice responded to a subsequent challenge injection of RBCs by formation of mainly IgM PFCs and serum antibody. Appearance of IgG PFCs and 2-ME resistant antibody was delayed several days in these carbon treated animals, indicating failure of a typical secondary response. The immune response of these animals was similar to that of a primary response of control animals to a single injection of red cells.

Reticulo-endothelial cell blockade with colloidal carbon suspensions interfered with development of a normal secondary type immune response to sheep red blood cells, as assayed on both the cellular and humoral levels. Fewer antibody PFCs, mainly 19S IgM but also 7S IgG, appeared in spleens of antigen primed mice treated with carbon 1–3 days prior to a challenge injection of red cells, as compared to control primed mice injected with erythrocytes alone. However, the peak day of antibody response was the same for both control and carbon treated animals.

Mice treated with carbon 1–3 days before secondary immunization had much lower peak serum titres, mostly susceptible to 2-ME inactivation.

The time of inoculation of carbon in relation to immunization was important since carbon treatment 1–3 days before secondary RBC immunization resulted in maximum suppression. Injection of carbon 5–7 days before resulted in only a slight effect, whereas injection 30 days before had no detectable effect. Injection of carbon simultaneously or after RBC injection had little effect.

The dose of carbon used for immunosuppression, as well as the concentration of sheep erythrocytes used for immunization affected the number of antibody PFCs and the serum titres in control as well as carbon treated animals.

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