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. 1969 Jul;17(1):71–76.

Double diffusion in agarose: precipitin lines which are not the result of antigen—antibody reactions

E Gardner, L T Rosenberg
PMCID: PMC1455940  PMID: 4978227

Abstract

Precipitin lines were observed in double diffusion tests between mouse gut tissue homogenates and serum from the same animal. Other tissues failed to produce this result. The line-forming components of the tissue are lipoidal while serum reactants are free of lipid. Purified γ-globulin, IgG, does not react with tissue constituents. An IgM component can be identified as one reactant. Lipopolysaccharides extracted from E. coli 014, Forssman antigen and Kidd—Friedewald antigen could not be demonstrated as reactants. It appears that certain tissue components which may be lipoproteins, and lipid free serum components, one of which is IgM, react in a way which resembles immunological interactions but which are believed not to be so.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dalmasso A. P., Müller-Eberhard H. J. Hemolytic activity of lipoprotein-depleted serum and the effect of certain anions on complement. J Immunol. 1966 Nov;97(5):680–685. [PubMed] [Google Scholar]
  2. Dumonde D. C. Tissue-specific antigens. Adv Immunol. 1966;5:245–412. doi: 10.1016/s0065-2776(08)60275-8. [DOI] [PubMed] [Google Scholar]
  3. Furth J., Kabat E. A. ASSOCIATION OF THE HETEROGENETIC ANTIGEN WITH A MATERIAL IN NORMAL AND TUMOR TISSUES SEDIMENTABLE AT HIGH SPEED. Science. 1940 May 17;91(2368):483–485. doi: 10.1126/science.91.2368.483. [DOI] [PubMed] [Google Scholar]
  4. Goldstein I. J., So L. L. Protein-carbonhydrate interaction. 3. Agar gel-diffusion studies on the interaction of Concanavalin A, a lectin isolated from jack bean, with polysaccharides. Arch Biochem Biophys. 1965 Aug;111(2):407–414. doi: 10.1016/0003-9861(65)90203-1. [DOI] [PubMed] [Google Scholar]
  5. KUNIN C. M., BEARD M. V. SEROLOGICAL STUDIES OF O ANTIGENS OF ESCHERICHIA COLI BY MEANS OF THE HEMAGGLUTINATION TEST. J Bacteriol. 1963 Mar;85:541–548. doi: 10.1128/jb.85.3.541-548.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Künzel W., Bundschuh G. Durch Methämoglobin bedingte "Präzipitation" von Humanserumeiweiss im Albuminbereich. Z Immunitatsforsch Allerg Klin Immunol. 1968 Mar;135(1):102–110. [PubMed] [Google Scholar]
  7. LEONARD C. G., THORNE C. B. Studies on the non-specific precipitation of basic serum proteins with gamma-glutamyl polypeptides. J Immunol. 1961 Aug;87:175–188. [PubMed] [Google Scholar]
  8. Leon M. A. Concanavalin A reaction with human normal immunoglobulin G and myeloma immunoglobulin G. Science. 1967 Dec 8;158(3806):1325–1326. doi: 10.1126/science.158.3806.1325. [DOI] [PubMed] [Google Scholar]
  9. PEETOOM F., ROSE N., RUDDY S., MICHELI A., GRABAR P. [Study of hemolysates of human erythrocytes. II. Presence in normal serums of a substance precipitating the hemolysate]. Ann Inst Pasteur (Paris) 1960 Feb;98:252–260. [PubMed] [Google Scholar]
  10. TACHIBANA D. K., ROSENBERG L. T. CHARACTERIZATION OF A GENETICALLY IDENTIFIABLE COMPONENT OF COMPLEMENT IN MICE. Folia Microbiol (Praha) 1964 May;14:129–144. doi: 10.1007/BF02873998. [DOI] [PubMed] [Google Scholar]
  11. WILSON J. F., WARREN G. Precipitin reaction between serum and lysed erythrocytes. J Clin Pathol. 1962 Jan;15:40–43. doi: 10.1136/jcp.15.1.40. [DOI] [PMC free article] [PubMed] [Google Scholar]

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