Abstract
Immunological memory and antibody formation appear to be properties of different cell lines. This follows from a demonstration that cells from spleens of mice primed with sheep erythrocytes are capable of inducing normally unresponsive bone marrow cells to produce specific antibody.
Primed spleen cells, taken from CBA or CBAT6T6 mice, were cultured for 5 or 6 days with an excess of bone marrow cells of the opposite chromosome type, and with antigen, in lethally irradiated, histocompatible mice. The tissue of origin of antibody-plaque-forming cells from the spleens of the irradiated mice was then identified from chromosome spreads of individual plaque-forming cells. After 5 days of culture, equal numbers of spleen and bone marrow cells were found producing antibody. At 6 days nearly all of the plaque-forming cells were from the bone marrow. Plaque-forming cells of bone marrow origin were also identified, using an immunogenetic technique, when semi-allogeneic mixtures of spleen and bone marrow cells were cultured together.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ADLER F. L. STUDIES ON MOUSE ANTIBODIES. I. THE RESPONSE TO SHEEP RED CELLS. J Immunol. 1965 Jul;95:26–38. [PubMed] [Google Scholar]
- Claman H. N., Chaperon E. A., Triplett R. F. Immunocompetence of transferred thymus-marrow cell combinations. J Immunol. 1966 Dec;97(6):828–832. [PubMed] [Google Scholar]
- Cunningham A. J. Studies on the cellular basis of IgM immunological memory. Immunology. 1969 May;16(5):621–632. [PMC free article] [PubMed] [Google Scholar]
- Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
- FRIEDMAN H. DISTRIBUTION OF ANTIBODY PLAQUE FORMING CELLS IN VARIOUS TISSUES OF SEVERAL STRAINS OF MICE INJECTED WITH SHEEP ERYTHROCYTES. Proc Soc Exp Biol Med. 1964 Nov;117:526–530. doi: 10.3181/00379727-117-29628. [DOI] [PubMed] [Google Scholar]
- Harris S., Harris T. N., Ogburn C. A. In vitro demonstration of suppressive antibody in mouse antisera vs. allogeneic spleen cells. J Immunol. 1967 Aug;99(2):447–453. [PubMed] [Google Scholar]
- Henry C., Jerne N. K. Competition of 19S and 7S antigen receptors in the regulation of the primary immune response. J Exp Med. 1968 Jul 1;128(1):133–152. doi: 10.1084/jem.128.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nossal G. J., Cunningham A., Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. 3. Chromosomal marker analysis of single antibody-forming cells in reconstituted, irradiated, or thymectomized mice. J Exp Med. 1968 Oct 1;128(4):839–853. doi: 10.1084/jem.128.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Radovich J., Hemingsen H., Talmage D. W. The enhancing effect of bone marrow cells on the immune response of irradiated mice reconstituted with spleen cells from normal and immunized donors. J Immunol. 1968 Apr;100(4):756–759. [PubMed] [Google Scholar]
- Sercarz E. E., Byers V. S. The X-Y-Z scheme of immunocyte maturation. 3. Early IgM memory and the nature of the memory cell. J Immunol. 1967 Apr;98(4):836–843. [PubMed] [Google Scholar]