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. 1997 Mar;90(3):376–382. doi: 10.1111/j.1365-2567.1997.00376.x

The antigen receptor complex on cord B lymphocytes.

P J Macardle 1, H Weedon 1, M Fusco 1, S Nobbs 1, J Ridings 1, L Flego 1, D M Roberton 1, H Zola 1
PMCID: PMC1456600  PMID: 9155644

Abstract

The neonatal immune system responds to a restricted range of antigens, producing largely IgM antibody of low affinity. Comparison of the components of the B-cell antigen receptor complex shows significantly elevated membrane levels of IgM in neonatal B cells, compared with adult cells. CD79, which acts as the signal transducer for membrane immunoglobulin, is elevated in parallel with IgM, while IgD is elevated to a lesser degree. CD19, CD21, CD22 and CD81, which are all involved in transmitting activation signals when immunoglobulin is engaged, are not elevated. CD32, which is involved in negative regulation of activation, is present at reduced levels on cord B cells. The elevation of B-cell membrane IgM persists during infancy. Neonatal B cells respond in vitro to interleukin-4 (IL-4) by further elevation of membrane IgM levels. The elevated level of membrane IgM may make neonatal B cells easier to trigger by low concentrations of antigen, but in vitro activation and immunoglobulin modulation experiments did not show significant differences between cord and adult B-cell responses to anti-IgM.

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Selected References

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  1. Allison A. C., Nawata Y. Cytokines mediating the proliferation and differentiation of B-1 lymphocytes and their role in ontogeny and phylogeny. Ann N Y Acad Sci. 1992 May 4;651:200–219. doi: 10.1111/j.1749-6632.1992.tb24616.x. [DOI] [PubMed] [Google Scholar]
  2. Barr I. G., Hancock W. W., Kraft N., Toh B. H., Atkins R. C. PHM 14: a novel monoclonal antibody that reacts with both normal and neoplastic human B cells but not B-CLL. Scand J Haematol. 1984 Aug;33(2):187–196. doi: 10.1111/j.1600-0609.1984.tb02395.x. [DOI] [PubMed] [Google Scholar]
  3. Bhat N. M., Kantor A. B., Bieber M. M., Stall A. M., Herzenberg L. A., Teng N. N. The ontogeny and functional characteristics of human B-1 (CD5+ B) cells. Int Immunol. 1992 Feb;4(2):243–252. doi: 10.1093/intimm/4.2.243. [DOI] [PubMed] [Google Scholar]
  4. Carter R. H., Fearon D. T. CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 1992 Apr 3;256(5053):105–107. doi: 10.1126/science.1373518. [DOI] [PubMed] [Google Scholar]
  5. DeFranco A. L. Structure and function of the B cell antigen receptor. Annu Rev Cell Biol. 1993;9:377–410. doi: 10.1146/annurev.cb.09.110193.002113. [DOI] [PubMed] [Google Scholar]
  6. Durandy A., Thuillier L., Forveille M., Fischer A. Phenotypic and functional characteristics of human newborns' B lymphocytes. J Immunol. 1990 Jan 1;144(1):60–65. [PubMed] [Google Scholar]
  7. Engel P., Zhou L. J., Ord D. C., Sato S., Koller B., Tedder T. F. Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule. Immunity. 1995 Jul;3(1):39–50. doi: 10.1016/1074-7613(95)90157-4. [DOI] [PubMed] [Google Scholar]
  8. Fearon D. T., Carter R. H. The CD19/CR2/TAPA-1 complex of B lymphocytes: linking natural to acquired immunity. Annu Rev Immunol. 1995;13:127–149. doi: 10.1146/annurev.iy.13.040195.001015. [DOI] [PubMed] [Google Scholar]
  9. Gathings W. E., Kubagawa H., Cooper M. D. A distinctive pattern of B cell immaturity in perinatal humans. Immunol Rev. 1981;57:107–126. doi: 10.1111/j.1600-065x.1981.tb00444.x. [DOI] [PubMed] [Google Scholar]
  10. Golding B., Muchmore A. V., Blaese R. M. Newborn and Wiskott-Aldrich patient B cells can be activated by TNP-Brucella abortus: evidence that TNP-Brucella abortus behaves as a T-independent type 1 antigen in humans. J Immunol. 1984 Dec;133(6):2966–2971. [PubMed] [Google Scholar]
  11. Griffioen A. W., Franklin S. W., Zegers B. J., Rijkers G. T. Expression and functional characteristics of the complement receptor type 2 on adult and neonatal B lymphocytes. Clin Immunol Immunopathol. 1993 Oct;69(1):1–8. doi: 10.1006/clin.1993.1142. [DOI] [PubMed] [Google Scholar]
  12. Haynes B. F., Hemler M. E., Mann D. L., Eisenbarth G. S., Shelhamer J., Mostowski H. S., Thomas C. A., Strominger J. L., Fauci A. S. Characterization of a monoclonal antibody (4F2) that binds to human monocytes and to a subset of activated lymphocytes. J Immunol. 1981 Apr;126(4):1409–1414. [PubMed] [Google Scholar]
  13. Johnstone A. P., Millard R. E. Quantitation of immunoglobulin isotypes in chronic lymphocytic leukaemic cells compared with normal adult and neonatal lymphocytes. Int Arch Allergy Appl Immunol. 1985;76(4):313–317. doi: 10.1159/000233713. [DOI] [PubMed] [Google Scholar]
  14. Kuritani T., Cooper M. D. Human b-cell differentiation. I. Analysis of immunoglobulin heavy chain switching using monoclonal anti-immunoglobulin M, G, and A antibodies and pokeweed mitogen-induced plasma cell differentiation. J Exp Med. 1982 Mar 1;155(3):839–851. doi: 10.1084/jem.155.3.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ledbetter J. A., Shu G., Gallagher M., Clark E. A. Augmentation of normal and malignant B cell proliferation by monoclonal antibody to the B cell-specific antigen BP50 (CDW40). J Immunol. 1987 Feb 1;138(3):788–794. [PubMed] [Google Scholar]
  16. Loken M. R., Brosnan J. M., Bach B. A., Ault K. A. Establishing optimal lymphocyte gates for immunophenotyping by flow cytometry. Cytometry. 1990;11(4):453–459. doi: 10.1002/cyto.990110402. [DOI] [PubMed] [Google Scholar]
  17. Miyawaki T., Moriya N., Nagaoki T., Taniguchi N. Maturation of B-cell differentiation ability and T-cell regulatory function in infancy and childhood. Immunol Rev. 1981;57:61–87. doi: 10.1111/j.1600-065x.1981.tb00442.x. [DOI] [PubMed] [Google Scholar]
  18. Nicholas R., Sinclair S. C. Regulation of the immune response. I. Reduction in ability of specific antibody to inhibit long-lasting IgG immunological priming after removal of the Fc fragment. J Exp Med. 1969 Jun 1;129(6):1183–1201. doi: 10.1084/jem.129.6.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pastorelli G., Rousset F., Pène J., Peronne C., Roncarolo M. G., Tovo P. A., de Vries J. E. Cord blood B cells are mature in their capacity to switch to IgE-producing cells in response to interleukin-4 in vitro. Clin Exp Immunol. 1990 Oct;82(1):114–119. doi: 10.1111/j.1365-2249.1990.tb05413.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Reth M. Antigen receptors on B lymphocytes. Annu Rev Immunol. 1992;10:97–121. doi: 10.1146/annurev.iy.10.040192.000525. [DOI] [PubMed] [Google Scholar]
  21. Ridge J. P., Fuchs E. J., Matzinger P. Neonatal tolerance revisited: turning on newborn T cells with dendritic cells. Science. 1996 Mar 22;271(5256):1723–1726. doi: 10.1126/science.271.5256.1723. [DOI] [PubMed] [Google Scholar]
  22. Sato S., Steeber D. A., Tedder T. F. The CD19 signal transduction molecule is a response regulator of B-lymphocyte differentiation. Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11558–11562. doi: 10.1073/pnas.92.25.11558. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shields J. G., Armitage R. J., Jamieson B. N., Beverley P. C., Callard R. E. Increased expression of surface IgM but not IgD or IgG on human B cells in response to IL-4. Immunology. 1989 Feb;66(2):224–227. [PMC free article] [PubMed] [Google Scholar]
  24. Small T. N., Keever C., Collins N., Dupont B., O'Reilly R. J., Flomenberg N. Characterization of B cells in severe combined immunodeficiency disease. Hum Immunol. 1989 Jul;25(3):181–193. doi: 10.1016/0198-8859(89)90081-5. [DOI] [PubMed] [Google Scholar]
  25. Splawski J. B., Jelinek D. F., Lipsky P. E. Delineation of the functional capacity of human neonatal lymphocytes. J Clin Invest. 1991 Feb;87(2):545–553. doi: 10.1172/JCI115029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Strimas J. H., Chi D. S. Significance of IgE level in amniotic fluid and cord blood for the prediction of allergy. Ann Allergy. 1988 Aug;61(2):133–136. [PubMed] [Google Scholar]
  27. Timens W., Boes A., Rozeboom-Uiterwijk T., Poppema S. Immaturity of the human splenic marginal zone in infancy. Possible contribution to the deficient infant immune response. J Immunol. 1989 Nov 15;143(10):3200–3206. [PubMed] [Google Scholar]
  28. Tony H. P., Phillips N. E., Parker D. C. Role of membrane immunoglobulin (Ig) crosslinking in membrane Ig-mediated, major histocompatibility-restricted T cell-B cell cooperation. J Exp Med. 1985 Nov 1;162(5):1695–1708. doi: 10.1084/jem.162.5.1695. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Watson W., Oen K., Ramdahin R., Harman C. Immunoglobulin and cytokine production by neonatal lymphocytes. Clin Exp Immunol. 1991 Jan;83(1):169–174. doi: 10.1111/j.1365-2249.1991.tb05609.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Weis J. J., Tedder T. F., Fearon D. T. Identification of a 145,000 Mr membrane protein as the C3d receptor (CR2) of human B lymphocytes. Proc Natl Acad Sci U S A. 1984 Feb;81(3):881–885. doi: 10.1073/pnas.81.3.881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Wienands J., Reth M. Glycosyl-phosphatidylinositol linkage as a mechanism for cell-surface expression of immunoglobulin D. Nature. 1992 Mar 19;356(6366):246–248. doi: 10.1038/356246a0. [DOI] [PubMed] [Google Scholar]
  32. Yachie A., Konno A., Ohta K., Wada T., Seki H., Taniguchi N., Miyawaki T. Delineation of producing ability of IgG and IgA subclasses by naive B cells in newborn infants and adult individuals. Clin Exp Immunol. 1995 Oct;102(1):204–209. doi: 10.1111/j.1365-2249.1995.tb06657.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Zipf T. F., Lauzon G. J., Longenecker B. M. A monoclonal antibody detecting a 39,000 m.w. molecule that is present on B lymphocytes and chronic lymphocytic leukemia cells but is rare on acute lymphocytic leukemia blasts. J Immunol. 1983 Dec;131(6):3064–3072. [PubMed] [Google Scholar]
  34. Zola H., Flego L., Wong Y. T., Macardle P. J., Kenney J. S. Direct demonstration of membrane IL-1 alpha on the surface of circulating B lymphocytes and monocytes. J Immunol. 1993 Mar 1;150(5):1755–1762. [PubMed] [Google Scholar]
  35. Zola H., Fusco M., Macardle P. J., Flego L., Roberton D. Expression of cytokine receptors by human cord blood lymphocytes: comparison with adult blood lymphocytes. Pediatr Res. 1995 Sep;38(3):397–403. doi: 10.1203/00006450-199509000-00021. [DOI] [PubMed] [Google Scholar]
  36. Zola H., Macardle P. J., Flego L., Webster J. The expression of sub-population markers on B cells: a re-evaluation using high-sensitivity fluorescence flow cytometry. Dis Markers. 1991 Mar-Apr;9(2):103–118. [PubMed] [Google Scholar]
  37. van Tol M. J., Zijlstra J., Zegers B. J., Ballieux R. E. Antigen-induced plaque-forming cell responses in cultures of peripheral blood mononuclear cells of human neonates and infants. J Pediatr. 1984 Nov;105(5):738–744. doi: 10.1016/s0022-3476(84)80293-0. [DOI] [PubMed] [Google Scholar]

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