Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1978 Aug;35(2):391–395.

Stimulation of human peripheral blood lymphocytes by Tamm-Horsfall urinary glycoprotein.

J S Hunt, A R McGiven
PMCID: PMC1457265  PMID: 750375

Abstract

Tamm-Horsfall urinary glycoprotein (THP), prepared by salt precipitation of pooled urine from normal individuals, stimulated purified human peripheral blood lymphocytes (PBL) to undergo blastoid transformation. the response was measured by tritiated thymidine uptake into DNA after 6 days in culture. Several batches of THP stimulated, in varying degrees, all samples of PBL tested and the response approached that seen with the mitogens phytohaemagglutinin (PHA), Concanavalin A (Con A) and pokeweed mitogen (PWM) after 4 days in culture. The response usually exceeded that seen after 6 days with tuberculin purified protein derivative (PPD) in Mantoux positive lymphocyte donors.

Full text

PDF
391

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Böyum A. Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g. Scand J Clin Lab Invest Suppl. 1968;97:77–89. [PubMed] [Google Scholar]
  2. Cochrane A. M., Tsantoulos D. C., Moussouros A., McFarlane I. G., Eddleston A. L., Williams R. Lymphocyte cytotoxicity for kidney cells in renal tubular acidosis of autoimmune liver disease. Br Med J. 1976 Jul 31;2(6030):276–278. doi: 10.1136/bmj.2.6030.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fletcher A. P., McLaughlin J. E., Ratcliffe W. A., Woods D. A. The chemical composition and electron microscopic appearance of a protein derived from urinary casts. Biochim Biophys Acta. 1970 Aug 21;214(2):299–308. doi: 10.1016/0005-2795(70)90007-3. [DOI] [PubMed] [Google Scholar]
  4. Hanson L. A., Fasth A., Jodal U. Autoantibodies to Tamm-Horsfall protein, a tool for diagnosing the level of urinary-tract infection. Lancet. 1976 Jan 31;1(7953):226–228. doi: 10.1016/s0140-6736(76)91342-8. [DOI] [PubMed] [Google Scholar]
  5. KEUTEL H. J. LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHIQUE. J Histochem Cytochem. 1965 Mar;13:155–160. [PubMed] [Google Scholar]
  6. Lis H., Sharon N. The biochemistry of plant lectins (phytohemagglutinins). Annu Rev Biochem. 1973;42(0):541–574. doi: 10.1146/annurev.bi.42.070173.002545. [DOI] [PubMed] [Google Scholar]
  7. McKenzie J. K., McQueen E. G. Immunofluorescent localization of Tamm-Horsfall mucoprotein in human kidney. J Clin Pathol. 1969 May;22(3):334–339. doi: 10.1136/jcp.22.3.334. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McQueen E. G. The nature of urinary casts. J Clin Pathol. 1962 Jul;15(4):367–373. doi: 10.1136/jcp.15.4.367. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller J. B., Hsu R., Heinrikson R., Yachnin S. Extensive homology between the subunits of the phytohemagglutinin mitogenic proteins derived from Phaseolus vulgaris. Proc Natl Acad Sci U S A. 1975 Apr;72(4):1388–1391. doi: 10.1073/pnas.72.4.1388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Penhale W. J., Farmer A., Maccuish A. C., Irvine W. J. A rapid micro-method for the phytohaemagglutinin-induced human lymphocyte transformation test. Clin Exp Immunol. 1974 Sep;18(1):155–167. [PMC free article] [PubMed] [Google Scholar]
  11. Schenk E. A., Schwartz R. H., Lewis R. A. Tamm-Horsfall mucoprotein. I. Localization in the kidney. Lab Invest. 1971 Jul;25(1):92–95. [PubMed] [Google Scholar]
  12. TAMM I., BUGHER J. C., HORSFALL F. L., Jr Ultracentrifugation studies of a urinary mucoprotein which reacts with various viruses. J Biol Chem. 1955 Jan;212(1):125–133. [PubMed] [Google Scholar]
  13. TAMM I., HORSFALL F. L., Jr Characterization and separation of an inhibitor of viral hemagglutination present in urine. Proc Soc Exp Biol Med. 1950 May;74(1):106–108. [PubMed] [Google Scholar]
  14. Tsantoulas D. C., McFarlane I. G., Portmann B., Eddleston A. L., Williams R. Cell-mediated immunity to human Tamm-Horsfall glycoprotein in autoimmune liver disease with renal tubular acidosis. Br Med J. 1974 Nov 30;4(5943):491–494. doi: 10.1136/bmj.4.5943.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Waxdal M. J. Isolation, characterization, and biological activities of five mitogens from pokeweed. Biochemistry. 1974 Aug 27;13(18):3671–3677. doi: 10.1021/bi00715a008. [DOI] [PubMed] [Google Scholar]
  16. Yachnin S., Allen L. W., Baron J. M., Svenson R. H. The potentiation of phytohemagglutinin-induced lymphocyte transformation by cell-cell interaction; a matrix hypothesis. Cell Immunol. 1972 Apr;3(4):569–589. doi: 10.1016/0008-8749(72)90120-7. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES