Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1979 May;37(1):279–286.

Antibody-containing cell response in lymph of sheep after intra-intestinal infusion of ovalbumin with and without DEAE-dextran.

K J Beh
PMCID: PMC1457284  PMID: 572818

Abstract

The output of antibody-containing cells (ACC) was monitored in efferent ileal lymph after continuous infusion of ovalbumin into the ileum of sheep with and without the adjuvant DEAE-dextran. When ovalbumin was infused at the slow rate of 5 ml/h, maximum outputs of 2.9 x 10(5) and 2.4 x 10(5 ACC/h were observed on days 9 and 16 respectively. When infused at the faster rate of 15 ml/h, peak levels of 6.9 x 10(5) and 11.7 x 10(5) ACC/h were recorded on days 10 and 16 respectively. The maximum response was substantially enhanced when ovalbumin was infused simultaneously with DEAE-dextran when a mean output of 51.7 x 10(5) ACC/h occurred on day 10. With all treatments the distribution of ACC amongst various immunoglobulin classes was similar. During the first few days of the response IgM-specific ACC predominated and later IgG1-specific ACC were most abundant. Throughout the response a substantial proportion (10-81%) of ACC in efferent ileal lymph were IgA-specific.

Full text

PDF
283

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen W. D., Porter P. Localization of immunoglobulins in intestinal mucosa and the production of secretory antibodies in response to intraluminal administration of bacterial antigens in the preruminant calf. Clin Exp Immunol. 1975 Sep;21(3):407–418. [PMC free article] [PubMed] [Google Scholar]
  2. BATTY I., WARRACK G. H. Local antibody production in the mammary gland, spleen, uterus, vagina and appendix of the rabbit. J Pathol Bacteriol. 1955 Oct;70(2):355–363. doi: 10.1002/path.1700700212. [DOI] [PubMed] [Google Scholar]
  3. BUTLER W. T. HEMAGGLUTINATION STUDIES WITH FORMALINIZED ERYTHROCYTES. EFFECT OF BIS-DIAZO-BENZIDINE AND TANNIC ACID TREATMENT ON SENSITIZATION BY SOLUBLE ANTIGEN. J Immunol. 1963 May;90:663–671. [PubMed] [Google Scholar]
  4. Beh K. J., Lascelles A. K. Class specificity of intracellular and surface immunoglobulin of cells in popliteal and intestinal lymph from sheep. Aust J Exp Biol Med Sci. 1974 Jun;52(Pt 3):505–514. doi: 10.1038/icb.1974.49. [DOI] [PubMed] [Google Scholar]
  5. Beh K. J. The origin of IgA-containing cells in intestinal lymph of sheep. Aust J Exp Biol Med Sci. 1977 Jun;55(3):263–274. doi: 10.1038/icb.1977.21. [DOI] [PubMed] [Google Scholar]
  6. Bockman D. E., Cooper M. D. Pinocytosis by epithelium associated with lymphoid follicles in the bursa of Fabricius, appendix, and Peyer's patches. An electron microscopic study. Am J Anat. 1973 Apr;136(4):455–477. doi: 10.1002/aja.1001360406. [DOI] [PubMed] [Google Scholar]
  7. Carter P. B., Collins F. M. Peyer's patch responsiveness to Salmonella in mice. J Reticuloendothel Soc. 1975 Jan;17(1):38–46. [PubMed] [Google Scholar]
  8. Cooper G. N., Halliday W. J., Thonard J. C. Immunological reactivity associated with antigens in the intestinal tract of rats. J Pathol Bacteriol. 1967 Jan;93(1):223–233. doi: 10.1002/path.1700930122. [DOI] [PubMed] [Google Scholar]
  9. Crabbé P. A., Nash D. R., Bazin H., Eyssen D. V., Heremans J. F. Antibodies of the IgA type in intestinal plasma cells of germfree mice after oral or parenteral immunization with ferritin. J Exp Med. 1969 Oct 1;130(4):723–744. doi: 10.1084/jem.130.4.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Porter P., Kenworthy R., Noakes D. E., Allen W. D. Intestinal antibody secretion in the young pig in response to oral immunization with Escherichia coli. Immunology. 1974 Nov;27(5):841–853. [PMC free article] [PubMed] [Google Scholar]
  11. Robertson P. W., Cooper G. N. Immune responses in intestinal tissues to particulate antigens. Plaque-forming and rosette-forming cell responses in rats. Aust J Exp Biol Med Sci. 1972 Dec;50(6):703–714. doi: 10.1038/icb.1972.63. [DOI] [PubMed] [Google Scholar]
  12. Rothberg R. M., Kraft S. C., Farr R. S. Similarities between rabbit antibodies produced following ingestion of bovine serum albumin and following parenteral immunization. J Immunol. 1967 Feb;98(2):386–395. [PubMed] [Google Scholar]
  13. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  14. Warshaw A. L., Walker W. A., Cornell R., Isselbacher K. J. Small intestinal permeability to macromolecules. Transmission of horseradish peroxidase into mesenteric lymph and portal blood. Lab Invest. 1971 Dec;25(6):675–684. [PubMed] [Google Scholar]
  15. Wittmann G., Dietzschold B., Bauer K. Some investigations on the adjuvant mechanism of DEAE dextran. Arch Virol. 1975;47(3):225–235. doi: 10.1007/BF01317810. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES