Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1978 Dec;35(6):963–970.

Influence of a synthetic adjuvant (MDP) on qualitative and quantitative changes of serum globulins.

C Leclerc, F Audibert, L Chedid
PMCID: PMC1457442  PMID: 738768

Abstract

Administered to guinea-pig, a synthetic compound, N-acetyl-muramyl-L-alanyl-D-isoglutamine (MDP), has been previously shown to substitute for Mycobacteria in Freund's complete adjuvant. Moreover, MDP increases the humoral immune response even when administered in an aqueous solution to mice. In the present report, it was demonstrated that administered to guinea-pig in a water-in-oil emulsion, MDP or active analogues favoured the production of IgG2 antibodies against ovalbumin. In contrast, derivatives of MDP which had no adjuvant activity failed to induce this particular class of immunoglobulins. MDP without antigen, in contrast with LPS or FCA, did not induce changes in immunoglobulin levels in mice. Administered in mice with an antigen, MDP induces an increase of IgG1 although the immunoglobulin levels are lower than those observed after immunization with adjuvants injected in a water-in-oil emulsion.

Full text

PDF
965

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASKONAS B. A., HUMPHREY J. H. Formation of specific antibodies and gamma-globulin in vitro; a study of the synthetic ability of various tissues from rabbits immunized by different methods. Biochem J. 1958 Feb;68(2):252–261. doi: 10.1042/bj0680252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adam A., Ciorbaru R., Petit J. F., Lederer E. Isolation and properties of a macromolecular, water-soluble, immuno-adjuvant fraction from the cell wall of Mycobacterium smegmatis. Proc Natl Acad Sci U S A. 1972 Apr;69(4):851–854. doi: 10.1073/pnas.69.4.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adam A., Devys M., Souvannavong V., Lefrancier P., Choay J., Lederer E. Correlation of structure and adjuvant activity of N-acetyl muramyl-L-alanyl-D-isoglutamine (MDP), its derivatives and analogues. Anti-adjuvant and competition properties of stereoisomers. Biochem Biophys Res Commun. 1976 Sep 7;72(1):339–346. doi: 10.1016/0006-291x(76)90999-2. [DOI] [PubMed] [Google Scholar]
  4. Audibert F., Chédid L., Lefrancier P., Choay J. Distinctive adjuvanticity of synthetic analogs of mycobacterial water-soluble components. Cell Immunol. 1976 Feb;21(2):243–249. doi: 10.1016/0008-8749(76)90053-8. [DOI] [PubMed] [Google Scholar]
  5. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Barth W. F., McLaughlin C. L., Fahey J. L. The immunoglobulins of mice. VI. Response to immunization. J Immunol. 1965 Nov;95(5):781–790. [PubMed] [Google Scholar]
  7. Chedid L., Audibert F., Lefrancier P., Choay J., Lederer E. Modulation of the immune response by a synthetic adjuvant and analogs. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2472–2475. doi: 10.1073/pnas.73.7.2472. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Coe J. E. 7Sgamma-1 and 7Sgamma-2 antibody response in the mouse. I. Influence of strain, antigen and adjuvant. J Immunol. 1966 Apr;96(4):744–753. [PubMed] [Google Scholar]
  9. De Vos-Cloetens C., Minsart-Baleriaux V., Urbain-Vansanten G. Possible relationships between antibodies and non-specific immunoglobulins simultaneously induced after antigenic stimulation. Immunology. 1971 Jun;20(6):955–962. [PMC free article] [PubMed] [Google Scholar]
  10. Ellouz F., Adam A., Ciorbaru R., Lederer E. Minimal structural requirements for adjuvant activity of bacterial peptidoglycan derivatives. Biochem Biophys Res Commun. 1974 Aug 19;59(4):1317–1325. doi: 10.1016/0006-291x(74)90458-6. [DOI] [PubMed] [Google Scholar]
  11. Franzl R. E., McMaster P. D. The primary immune response in mice. I. The enhancement and suppression of hemolysin production by a bacterial endotoxin. J Exp Med. 1968 Jun 1;127(6):1087–1107. doi: 10.1084/jem.127.6.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gurvitch A. E., Nikolaeva A. Changes in the synthesis of antibiodies and non-specific immunoglobulins in the spleen perfused in vitro. Immunology. 1971 Dec;21(6):915–924. [PMC free article] [PubMed] [Google Scholar]
  13. Herbert W. J. The mode of action of mineral-oil emulsion adjuvants on antibody production in mice. Immunology. 1968 Mar;14(3):301–318. [PMC free article] [PubMed] [Google Scholar]
  14. Hirata A. A., Brandriss M. W. Passive hemagglutination procedures for protein and polysaccharide antigens using erythrocytes stabilized by aldehydes. J Immunol. 1968 Mar;100(3):641–646. [PubMed] [Google Scholar]
  15. Hiu I. J. Water-soluble and lipid-free fraction from BCG with adjuvant and antitumour activity. Nat New Biol. 1972 Aug 23;238(86):241–242. doi: 10.1038/newbio238241a0. [DOI] [PubMed] [Google Scholar]
  16. JOHNSON A. G., GAINES S., LANDY M. Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide. J Exp Med. 1956 Feb 1;103(2):225–246. doi: 10.1084/jem.103.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kotani S., Watanabe Y., Kinoshita F., Shimono T., Morisaki I. Immunoadjuvant activities of synthetic N-acetyl-muramyl-peptides or -amino acids. Biken J. 1975 Jun;18(2):105–111. [PubMed] [Google Scholar]
  18. LOWE J. S. SERUM PROTEIN CHANGES IN RATS WITH ARTHRITIS INDUCED BY MYCOBACTERIAL ADJUVANT. Biochem Pharmacol. 1964 Apr;13:633–641. doi: 10.1016/0006-2952(64)90050-4. [DOI] [PubMed] [Google Scholar]
  19. Lefrancier P., Choay J., Derrien M., Lederman I. Synthesis of N-acetyl-muramyl-L-alanyl-D-isoglutamine, an adjuvant of the immune response, and of some n-acetyl-muramyl-peptide analogs. Int J Pept Protein Res. 1977;9(4):249–257. doi: 10.1111/j.1399-3011.1977.tb03488.x. [DOI] [PubMed] [Google Scholar]
  20. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  21. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  22. Migliore-Samour D., Jollès P. A hydrosoluble, adjuvant-active mycobacterial "polysaccharide-peptidoglycan". Preparation by a simple extraction technique of the bacterial cells (strain Peurois). FEBS Lett. 1972 Sep 15;25(2):301–304. doi: 10.1016/0014-5793(72)80509-x. [DOI] [PubMed] [Google Scholar]
  23. Moticka E. J. The non-specific stimulation of immunoglobulin secretion following specific stimulation of the immune system. Immunology. 1974 Sep;27(3):401–412. [PMC free article] [PubMed] [Google Scholar]
  24. ROWLEY D., TURNER K. J. INCREASE IN MACROGLOBULIN ANTIBODIES OF MOUSE AND PIG FOLLOWING INJECTION OF BACTERIAL LIPOPOLYSACCHARIDE. Immunology. 1964 Jul;7:394–402. [PMC free article] [PubMed] [Google Scholar]
  25. Tam L. Q., Benedict A. A. Elevated 7S immunoglobulin and acute phase proteins in adjuvant-injected chickens. Proc Soc Exp Biol Med. 1975 Nov;150(2):340–346. doi: 10.3181/00379727-150-39032. [DOI] [PubMed] [Google Scholar]
  26. Urbain-Vansanten G. Concomitant synthesis, in separate cells, of non-reactive immunoglobulins and specific antibodies after immunization with tobacco mosaic virus. Immunology. 1970 Nov;19(5):783–797. [PMC free article] [PubMed] [Google Scholar]
  27. WHITE R. G., JENKINS G. C., WILKINSON P. C. The production of skin-sensitizing antibody in the guinea-pig. Int Arch Allergy Appl Immunol. 1963;22:156–165. doi: 10.1159/000229362. [DOI] [PubMed] [Google Scholar]
  28. Wilkinson P. C., White R. G. The role of mycobacteria and silica in the immunological response of the guinea-pig. Immunology. 1966 Sep;11(3):229–241. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES