Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1979 Oct;38(2):385–392.

The respiratory response of sensitized rats to challenge with antigen aerosols.

H Piechuta, M E Smith, N N Share, G Holme
PMCID: PMC1457924  PMID: 511221

Abstract

An experimental procedure was developed to evaluate the effects of challenging sensitized conscious rats with antigen aerosols. The challenge resulted in changes in the respiratory patterns which were antigen specific and mediated by IgE antibodies. The response was inducible in non-sensitized rats by passive administration of IgE-rich serum. Sprague-Dawley rats were heterogeneous with respect to the respiratory response. A proportion (20--70%) of each group had continuous dyspnoea and other symptoms similar to asthma; the other had only episodes of apnoea. Wistar and Long-Evans rats resembled Sprague-Dawley rats; Fischer 344 rats had apnoea only, even though they produced IgE antibodies. The type of response did not correlate with serum IgE levels. The respiratory responses were reduced by dexamethasone, disodium cromoglycate, epinephrine and theophylline. Rats that respond with dyspnoea may provide a useful experimental model of allergic asthma.

Full text

PDF
387

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BINAGHI R. A., BENACERRAF B. THE PRODUCTION OF ANAPHYLACTIC ANTIBODY IN THE RAT. J Immunol. 1964 Jun;92:920–926. [PubMed] [Google Scholar]
  2. Barch G. K., Talbott M. W. Allergic bronchoconstriction and its drug-induced reversal in anesthetized, ovalbumin-sensitized cats. Res Commun Chem Pathol Pharmacol. 1976 Apr;13(4):623–633. [PubMed] [Google Scholar]
  3. Carney I. F. IgE-mediated anaphylactic bronchoconstriction in the guinea pig and the effect of disodium cromoglycate. Int Arch Allergy Appl Immunol. 1976;50(3):322–328. doi: 10.1159/000231509. [DOI] [PubMed] [Google Scholar]
  4. Carswell F., Oliver J. Site of respiratory reaction in allergic rats challenged via the airways. Int Arch Allergy Appl Immunol. 1978;57(4):358–363. doi: 10.1159/000232125. [DOI] [PubMed] [Google Scholar]
  5. Church M. K., Collier H. O., James G. W. The inhibition by dexamethasone and disodium cromoglycate of anaphylactic bronchoconstriction in the rat. Br J Pharmacol. 1972 Sep;46(1):56–65. doi: 10.1111/j.1476-5381.1972.tb06848.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Church M. K. Correlation of anaphylactic bronchoconstriction with circulating reaginic antibody level and active cutaneous anaphylaxis in the rat. Immunology. 1975 Sep;29(3):527–534. [PMC free article] [PubMed] [Google Scholar]
  7. Faith R. E., Hessler J. R., Small P. A., Jr Respiratory allergy in the dog: induction by the respiratory route and the effect of passive antibody. Int Arch Allergy Appl Immunol. 1977;53(6):530–544. doi: 10.1159/000231795. [DOI] [PubMed] [Google Scholar]
  8. Farmer J. B., Richards I. M., Sheard P., Woods A. M. Mediators of passive lung anaphylaxis in the rat. Br J Pharmacol. 1975 Sep;55(1):57–64. doi: 10.1111/j.1476-5381.1975.tb07610.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gold W. M., Kessler G. F., Yu D. Y., Frick O. L. Pulmonary physiologic abnormalities in experimental asthma in dogs. J Appl Physiol. 1972 Oct;33(4):496–501. doi: 10.1152/jappl.1972.33.4.496. [DOI] [PubMed] [Google Scholar]
  10. KEELING J. E. The effects of ultra-violet radiation on Nippostrongylus muris. I. Irradiation of infective larvae: lethal and sublethal effects. Ann Trop Med Parasitol. 1960 Jun;54:182–191. doi: 10.1080/00034983.1960.11685974. [DOI] [PubMed] [Google Scholar]
  11. Kepron W., James J. M., Kirk B., Sehon A. H., Tse K. S. A canine model for reaginic hypersensitivity and allergic bronchoconstriction. J Allergy Clin Immunol. 1977 Jan;59(1):64–69. doi: 10.1016/0091-6749(77)90178-6. [DOI] [PubMed] [Google Scholar]
  12. Margni R. A., Hajos S. E. Guinea-pig reaginic antibody. II. Physicochemical and biological properties. Immunology. 1973 Aug;25(2):333–342. [PMC free article] [PubMed] [Google Scholar]
  13. Patterson R., Harris K. E., Suszko I. M., Roberts M. Reagin-mediated asthma in rhesus monkeys and relation to bronchial cell histamine release and airway reactivity to carbocholine. J Clin Invest. 1976 Mar;57(3):586–593. doi: 10.1172/JCI108314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Patterson R., Kelly J. F. Animal models of the asthmatic state. Annu Rev Med. 1974;25(0):53–68. doi: 10.1146/annurev.me.25.020174.000413. [DOI] [PubMed] [Google Scholar]
  15. Patterson R., Talbot C. A comparison of immediate-type respiratory reactions to immunologic and pharmacologic agents in rhesus monkeys. J Allergy Clin Immunol. 1972 May;49(5):292–300. doi: 10.1016/0091-6749(72)90097-8. [DOI] [PubMed] [Google Scholar]
  16. Stechschulte D. J., Orange R. P., Austen K. F. Immunochemical and biologic properties of rat IgE. I. Immunochemical identification of rat IgE. J Immunol. 1970 Nov;105(5):1082–1086. [PubMed] [Google Scholar]
  17. Stotland L. M., Share N. N. Active bronchial anaphylaxis in the rat. Can J Physiol Pharmacol. 1974 Dec;52(6):1114–1118. doi: 10.1139/y74-146. [DOI] [PubMed] [Google Scholar]
  18. Stotland L. M., Share N. N. Pharmacological studies on active bronchial anaphylaxis in the rat. Can J Physiol Pharmacol. 1974 Dec;52(6):1119–1125. doi: 10.1139/y74-147. [DOI] [PubMed] [Google Scholar]
  19. Taylor W. A., Francis D. H., Sheldon D., Roitt I. M. Anti-allergic actions of disodium cromoglycate and other drugs known to inhibit cyclic 3',5'-nucleotide phosphodiesterase. Int Arch Allergy Appl Immunol. 1974;47(2):175–193. doi: 10.1159/000231212. [DOI] [PubMed] [Google Scholar]
  20. Van Hout C. A., Johnson H. G. Synthesis of rat IgE by aerosol immunization. J Immunol. 1972 Mar;108(3):834–836. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES