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. 1980 Feb;39(2):231–237.

Immune mechanisms against canine distemper. III. Role of complement lysis in the immunity and persistent infection of canine distemper virus.

C K Ho, L A Babiuk
PMCID: PMC1457963  PMID: 7380468

Abstract

Antibody-mediated complement lysis of Vero cells and canine macrophages infected with canine distemper virus (CDV) was demonstratee in an in vitro 51Cr release assay. This cytolytic activity was found to be highly efficient and was optimal under conditions which favoured the capping of redistribution of surface viral antigens. A prozone was observed in the presence of high antibody concentration and could not be eliminated by repeated washings. By tagging antibody-coated target cells with 125I-labelled staphylococcus protein A, it was found that the extent of protein A binding was parallel to the degree of cytotoxicity suggesting that the mechanism of this prozone effect was similar to that of a precipitation test.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Babiuk L. A., Acres S. D., Rouse B. T. Solid-phase radioimmunoassay for detecting bovine (neonatal calf diarrhea) rotavirus antibody. J Clin Microbiol. 1977 Jul;6(1):10–15. doi: 10.1128/jcm.6.1.10-15.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ehrnst A. C. Characterization of measles virus-specific cytotoxic antibodies by use of a chronically infected cell line. J Immunol. 1975 Mar;114(3):1077–1082. [PubMed] [Google Scholar]
  3. Goudswaard J., van der Donk J. A., Noordzij A., van Dam R. H., Vaerman J. P. Protein A reactivity of various mammalian immunoglobulins. Scand J Immunol. 1978;8(1):21–28. doi: 10.1111/j.1365-3083.1978.tb00492.x. [DOI] [PubMed] [Google Scholar]
  4. HSIUNG G. D., STAFSETH H. J. Canine distemper. I. Bacteriological studies. Cornell Vet. 1952 Apr;42(2):223–231. [PubMed] [Google Scholar]
  5. Ho C. K., Babiuk L. A. A new plaque system for canine distemper: characteristics of the green strain of canine distemper virus. Can J Microbiol. 1979 Jun;25(6):680–685. doi: 10.1139/m79-098. [DOI] [PubMed] [Google Scholar]
  6. Ho C. K., Babiuk L. A. Immune mechanisms against canine distemper. I. Identification of K cell against canine distemper virus infected target cells in vitro. Immunology. 1979 May;37(1):231–239. [PMC free article] [PubMed] [Google Scholar]
  7. Ho C. K., Babiuk L. A. Isolation of various canine leucocytes and their characterization by surface marker analysis. Immunology. 1978 Nov;35(5):733–740. [PMC free article] [PubMed] [Google Scholar]
  8. Joseph B. S., Oldstone M. B. Immunologic injury in measles virus infection. II. Suppression of immune injury through antigenic modulation. J Exp Med. 1975 Oct 1;142(4):864–876. doi: 10.1084/jem.142.4.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kieling F., Bandlow G., Thomssen R. Cytotoxische Reaktionen an Sendai-virus infizierten Zellen. Arch Gesamte Virusforsch. 1972;36(1):123–132. [PubMed] [Google Scholar]
  10. Minagawa T., Yamada M. Studies on the persistent infection with measles virus in HeLa cells. 3. Immunolysis of cells in carrier state by anti-measles sera. Jpn J Microbiol. 1971 Jul;15(4):341–350. doi: 10.1111/j.1348-0421.1971.tb00590.x. [DOI] [PubMed] [Google Scholar]
  11. Norrby E., Vandvik B. Measles and multiple sclerosis. Proc R Soc Med. 1974 Nov;67(11):1129–1132. [PMC free article] [PubMed] [Google Scholar]
  12. Oldstone M. B., Tishon A. Immunologic injury in measles virus infection. IV. Antigens modulation and abrogation oflymphocyte lysis of virus-infected cells. Clin Immunol Immunopathol. 1978 Jan;9(1):55–62. doi: 10.1016/0090-1229(78)90120-4. [DOI] [PubMed] [Google Scholar]
  13. STARR S., BERKOVICH S. EFFECTS OF MEASLES, GAMMA-GLOBULIN-MODIFIED MEASLES AND VACCINE MEASLES ON THE TUBERCULIN TEST. N Engl J Med. 1964 Feb 20;270:386–391. doi: 10.1056/NEJM196402202700802. [DOI] [PubMed] [Google Scholar]
  14. Summers B. A., Greisen H. A., Appel M. J. Possible initiation of viral encephalomyelitis in dogs by migrating lymphocytes infected with distemper virus. Lancet. 1978 Jul 22;2(8082):187–189. doi: 10.1016/s0140-6736(78)91924-4. [DOI] [PubMed] [Google Scholar]
  15. Wear D. J., Rapp F. Latent measles virus infection of the hamster central nervous system. J Immunol. 1971 Dec;107(6):1593–1598. [PubMed] [Google Scholar]

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