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. 1981 Feb;42(2):191–199.

Release of platelet-activating factor (PAF) and histamine. II. The cellular origin of human PAF: monocytes, polymorphonuclear neutrophils and basophils.

G Camussi, M Aglietta, R Coda, F Bussolino, W Piacibello, C Tetta
PMCID: PMC1458080  PMID: 6161885

Abstract

The origin of platelet activating factor (PAF) from human leucocytes was investigated. Purified monocytes release PAF passively at pH 10.6, when challenged with Ionophore A 23187 or under phagocytic stimuli. Pure preparations of polymorphonuclear neutrophils liberate PAF passively, when challenged with C5a, neutrophil cationic proteins (CP), their carboxypeptidase B derived products (C5a des Arg, CP des Arg) or under phagocytic stimuli. Basophil rich buffy coat cells release PAF when challenged with C5a, CP, anti-IgE (in low amount) or Synacthen concomitantly with basophil degranulation and histamine release. Electron microscopy studies, carried out on Synacthen-stimulated basophil rich buffy coat, provide morphological evidence for platelet-basophil interaction. In conclusion our data demonstrate that PAF can be released from different leucocyte populations. However, the stimuli able to trigger such release appear to have some specificity for the cell target.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bang N. U., Heidenreich R. O., Trygstad C. W. Plasma protein requirements for human platelet aggregation. Ann N Y Acad Sci. 1972 Oct 27;201:280–299. doi: 10.1111/j.1749-6632.1972.tb16305.x. [DOI] [PubMed] [Google Scholar]
  2. Benveniste J., Camussi J., Polonsky J. Platelet-activating factor. Monogr Allergy. 1977;12:138–142. [PubMed] [Google Scholar]
  3. Benveniste J., Egido J., Gutierrez Millet V., Camussi G. Detection of immediate hypersensitivity in rabbits by direct basophil degranulation. J Allergy Clin Immunol. 1977 Apr;59(4):271–279. doi: 10.1016/0091-6749(77)90047-1. [DOI] [PubMed] [Google Scholar]
  4. Benveniste J., Henson P. M., Cochrane C. G. Leukocyte-dependent histamine release from rabbit platelets. The role of IgE, basophils, and a platelet-activating factor. J Exp Med. 1972 Dec 1;136(6):1356–1377. doi: 10.1084/jem.136.6.1356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benveniste J., Le Couedic J. P., Polonsky J., Tence M. Structural analysis of purified platelet-activating factor by lipases. Nature. 1977 Sep 8;269(5624):170–171. doi: 10.1038/269170a0. [DOI] [PubMed] [Google Scholar]
  6. Benveniste J., Tencé M., Varenne P., Bidault J., Boullet C., Polonsky J. Semi-synthèse et structure proposée du facteur activant les plaquettes (P.A.F.): PAF-acether, un alkyl ether analogue de la lysophosphatidylcholine. C R Seances Acad Sci D. 1979 Nov 26;289(14):1037–1040. [PubMed] [Google Scholar]
  7. Bokisch V. A., Müller-Eberhard H. J. Anaphylatoxin inactivator of human plasma: its isolation and characterization as a carboxypeptidase. J Clin Invest. 1970 Dec;49(12):2427–2436. doi: 10.1172/JCI106462. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Brittinger G., Hirschhorn R., Douglas S. D., Weissmann G. Studies on lysosomes. XI. Characterization of a hydrolase-rich fraction from human lymphocytes. J Cell Biol. 1968 May;37(2):394–411. doi: 10.1083/jcb.37.2.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Bussolino F., Benveniste J. Pharmacological modulation of platelet-activating factor (PAF) release from rabbit leucocytes. I. Role of cAMP. Immunology. 1980 Jul;40(3):367–376. [PMC free article] [PubMed] [Google Scholar]
  10. Camussi G., Mencia-Huerta J. M., Benveniste J. Release of platelet-activating factor and histamine. I. Effect of immune complexes, complement and neutrophils on human and rabbit mastocytes and basophils. Immunology. 1977 Oct;33(4):523–534. [PMC free article] [PubMed] [Google Scholar]
  11. Camussi G., Tetta C., Segoloni G., Vercellone A. Immunological mechanisms of human platelet involvement. Ric Clin Lab. 1978 Oct-Dec;8(4):262–272. [PubMed] [Google Scholar]
  12. Chignard M., Le Couedic J. P., Tence M., Vargaftig B. B., Benveniste J. The role of platelet-activating factor in platelet aggregation. Nature. 1979 Jun 28;279(5716):799–800. doi: 10.1038/279799a0. [DOI] [PubMed] [Google Scholar]
  13. Cochrane C. G., Müller-Eberhard H. J. The derivation of two distinct anaphylatoxin activities from the third and fifth components of human complement. J Exp Med. 1968 Feb 1;127(2):371–386. doi: 10.1084/jem.127.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Demopoulos C. A., Pinckard R. N., Hanahan D. J. Platelet-activating factor. Evidence for 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine as the active component (a new class of lipid chemical mediators). J Biol Chem. 1979 Oct 10;254(19):9355–9358. [PubMed] [Google Scholar]
  15. Henson P. M., Pinckard R. N. Basophil-derived platelet-activating factor (PAF) as an in vivo mediator of acute allergic reactions: demonstration of specific desensitization of platelets to PAF during IgE-induced anaphylaxis in the rabbit. J Immunol. 1977 Dec;119(6):2179–2184. [PubMed] [Google Scholar]
  16. Jaques R., Brugger M. Synthetic polypeptides related to corticotrophin acting as histamine liberators. Pharmacology. 1969;2(6):361–370. doi: 10.1159/000136040. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. Lewis R. A., Goetzl E. J., Wasserman S. I., Valone F. H., Rubin R. H., Austen K. F. The release of four mediators of immediate hypersensitivity from human leukemic basophils. J Immunol. 1975 Jan;114(1 Pt 1):87–92. [PubMed] [Google Scholar]
  19. Lotner G. Z., Lynch J. M., Betz S. J., Henson P. M. Human neutrophil-derived platelet activating factor. J Immunol. 1980 Feb;124(2):676–684. [PubMed] [Google Scholar]
  20. Lynch J. M., Lotner G. Z., Betz S. J., Henson P. M. The release of a platelet-activating factor by stimulated rabbit neutrophils. J Immunol. 1979 Sep;123(3):1219–1226. [PubMed] [Google Scholar]
  21. Mencia-Huerta J. M., Benveniste J. Platelet-activating factor and macrophages. I. Evidence for the release from rat and mouse peritoneal macrophages and not from mastocytes. Eur J Immunol. 1979 May;9(5):409–415. doi: 10.1002/eji.1830090512. [DOI] [PubMed] [Google Scholar]
  22. PROCKOP D. J., DAVIDSON W. D. A STUDY OF URINARY AND SERUM LYSOZYME IN PATIENTS WITH RENAL DISEASE. N Engl J Med. 1964 Feb 6;270:269–274. doi: 10.1056/NEJM196402062700602. [DOI] [PubMed] [Google Scholar]
  23. RABINOWITZ Y. SEPARATION OF LYMPHOCYTES, POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES ON GLASS COLUMNS, INCLUDING TISSUE CULTURE OBSERVATIONS. Blood. 1964 Jun;23:811–828. [PubMed] [Google Scholar]
  24. Ruff F., Saindelle A., Dutripon E., Parrot J. L. Continuous automatic fluorometric evaluation of total blood histamine. Nature. 1967 Apr 15;214(5085):279–281. doi: 10.1038/214279b0. [DOI] [PubMed] [Google Scholar]
  25. Shaw J. O., Henson P. M. The binding of rabbit basophil-derived platelet-activating factor to rabbit platelets. Am J Pathol. 1980 Mar;98(3):791–810. [PMC free article] [PubMed] [Google Scholar]

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