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. 1981 Mar;42(3):379–383.

Diverging effects of chemotactic serum peptides and synthetic f-Met-Leu-Phe on neutrophil locomotion and adhesion.

H U Keller, J H Wissler, B Damerau
PMCID: PMC1458440  PMID: 7203527

Abstract

The chemotactic serum peptide preparations CAT 1.6.1. and C5adesArg induced marked directional locomotion over a wide concentration range without significant effects on random locomotion and adhesion of human neutrophils in Gey's solution containing 2% HSA. In contrast, f-Met-Leu-Phe produced marked negative chemokinetic effects and its capacity to induce directional locomotion was more limited with respect to magnitude and concentration range. The negative chemokinetic effect of f-Met-Leu-Phe correlated closely with increased spreading and cell adhesion.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becker E. L., Sigman M., Oliver J. M. Superoxide production induced in rabbit polymorphonuclear leukocytes by synthetic chemotactic peptides and A23187. Am J Pathol. 1979 Apr;95(1):81–97. [PMC free article] [PubMed] [Google Scholar]
  2. Cramer E. B., Gallin J. I. Localization of submembranous cations to the leading end of human neutrophils during chemotaxis. J Cell Biol. 1979 Aug;82(2):369–379. doi: 10.1083/jcb.82.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fehr J., Jacob H. S. In vitro granulocyte adherence and in vivo margination: two associated complement-dependent functions. Studies based on the acute neutropenia of filtration leukophoresis. J Exp Med. 1977 Sep 1;146(3):641–652. doi: 10.1084/jem.146.3.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Keller H. U., Barandun S., Kistler P., Ploem J. S. Locomotion and adhesion of neutrophil granulocytes. Effects of albumin, fibrinogen and gamma globulins studied by reflection contrast microscopy. Exp Cell Res. 1979 Sep;122(2):351–362. doi: 10.1016/0014-4827(79)90311-2. [DOI] [PubMed] [Google Scholar]
  5. Keller H. U., Gerber H., Hess M. W., Cottier H. Studies on the regulation of the neutrophil chemotactic response using a rapid and reliable method for measuring random migration and chemotaxis of neutrophil granulocytes. Agents Actions. 1976 Feb;6(1-3):326–339. doi: 10.1007/BF01972250. [DOI] [PubMed] [Google Scholar]
  6. Keller H. U., Wissler J. H., Hess M. W., Cottier H. Distinct chemokinetic and chemotactic responses in neutrophil granulocytes. Eur J Immunol. 1978 Jan;8(1):1–7. doi: 10.1002/eji.1830080102. [DOI] [PubMed] [Google Scholar]
  7. Kreutzer D. L., O'Flaherty J. T., Orr W., Showell H. J., Ward P. A., Becker E. L. Quantitative comparisons of various biological responses of neutrophils to different active and inactive chemotactic factors. Immunopharmacology. 1978 Dec;1(1):39–47. doi: 10.1016/0162-3109(78)90007-3. [DOI] [PubMed] [Google Scholar]
  8. Naccache P. H., Showell H. J., Becker E. L., Sha'afi R. I. Changes in ionic movements across rabbit polymorphonuclear leukocyte membranes during lysosomal enzyme release. Possible ionic basis for lysosomal enzyme release. J Cell Biol. 1977 Dec;75(3):635–649. doi: 10.1083/jcb.75.3.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. O'Flaherty J. T., Kreutzer D. L., Ward P. A. The influence of chemotactic factors on neutrophil adhesiveness. Inflammation. 1978 Mar;3(1):37–48. doi: 10.1007/BF00917320. [DOI] [PubMed] [Google Scholar]
  10. Smith C. W., Hollers J. C., Patrick R. A., Hassett C. Motility and adhesiveness in human neutrophils. Effects of chemotactic factors. J Clin Invest. 1979 Feb;63(2):221–229. doi: 10.1172/JCI109293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith R. P., Lackie J. M., Wilkinson P. C. The effects of chemotactic factors on the adhesiveness of rabbit neutrophil granulocytes. Exp Cell Res. 1979 Aug;122(1):169–177. doi: 10.1016/0014-4827(79)90571-8. [DOI] [PubMed] [Google Scholar]
  12. Spilberg I., Mandell B., Mehta J., Sullivan T., Simchowitz L. Dissociation of the neutrophill functions of exocytosis and chemotaxis. J Lab Clin Med. 1978 Aug;92(2):297–302. [PubMed] [Google Scholar]
  13. Wissler J. H. Chemistry and biology of the anaphylatoxin related serum peptide system. I. Purification, crystallization and properties of classical anaphylatoxin from rat serum. Eur J Immunol. 1972 Feb;2(1):73–83. doi: 10.1002/eji.1830020115. [DOI] [PubMed] [Google Scholar]
  14. Zigmond S. H. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J Cell Biol. 1977 Nov;75(2 Pt 1):606–616. doi: 10.1083/jcb.75.2.606. [DOI] [PMC free article] [PubMed] [Google Scholar]

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