Abstract
1. Effects of the platelet secretagogues thrombin and Concanavalin A (Con A) on shape change and release have been studied under conditions where external Ca2+ and ADP have been controlled. 2. In the absence of detectable aggregation both Con A- and thrombin-induced release of serotonin are markedly dependent upon extracellular calcium. 3. Con A, unlike thrombin, does not produce aggregation in calcium-supplemented medium. 4. The above property of Con A suggests that, in combination with added creatine phosphate and creatine phosphokinase, this ligand will be of value in the analysis of calcium-dependent release in platelets.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- ASTER R. H., JANDL J. H. PLATELET SEQUESTRATION IN MAN. I. METHODS. J Clin Invest. 1964 May;43:843–855. doi: 10.1172/JCI104970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BORN G. V., CROSS M. J. THE AGGREGATION OF BLOOD PLATELETS. J Physiol. 1963 Aug;168:178–195. doi: 10.1113/jphysiol.1963.sp007185. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Born G. V. Observations on the change in shape of blood platelets brought about by adenosine diphosphate. J Physiol. 1970 Aug;209(2):487–511. doi: 10.1113/jphysiol.1970.sp009176. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cronberg S., Caen J. P. Release reaction in washed platelet suspensions induced by kaolin and other particles. Scand J Haematol. 1971;8(2):151–160. doi: 10.1111/j.1600-0609.1971.tb01966.x. [DOI] [PubMed] [Google Scholar]
- Feinman R. D., Detwiler T. C. Platelet secretion induced by divalent cation ionophores. Nature. 1974 May 10;249(453):172–173. doi: 10.1038/249172a0. [DOI] [PubMed] [Google Scholar]
- Feinstein M. B., Fraser C. Human platelet secretion and aggregation induced by calcium ionophores. Inhibition by PGE1 and dibutyryl cyclic AMP. J Gen Physiol. 1975 Nov;66(5):561–581. doi: 10.1085/jgp.66.5.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Greenberg J. H., Jamieson G. A. The effects of various lectins on platelet aggregation and release. Biochim Biophys Acta. 1974 Apr 29;345(2):231–242. doi: 10.1016/0005-2736(74)90261-2. [DOI] [PubMed] [Google Scholar]
- Jenkins C. S., Packham M. A., Kinlough-Rathbone R. L., Mustard J. F. Interactions of polylysine with platelets. Blood. 1971 Apr;37(4):395–412. [PubMed] [Google Scholar]
- Lis H., Sharon N. The biochemistry of plant lectins (phytohemagglutinins). Annu Rev Biochem. 1973;42(0):541–574. doi: 10.1146/annurev.bi.42.070173.002545. [DOI] [PubMed] [Google Scholar]
- MACMILLAN D. C., OLIVER M. F. THE INITIAL CHANGES IN PLATELET MORPHOLOGY FOLLOWING THE ADDITION OF ADENOSINE DIPHOSPHATE. J Atheroscler Res. 1965 Jul-Aug;5(4):440–444. doi: 10.1016/s0368-1319(65)80081-3. [DOI] [PubMed] [Google Scholar]
- MORSE E. E., JACKSON D. P., CONLEY C. L. ROLE OF PLATELET FIBRINOGEN IN THE REACTIONS OF PLATELETS TO THROMBIN. J Clin Invest. 1965 May;44:809–816. doi: 10.1172/JCI105193. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Massini P., Lüscher E. F. Some effects of ionophores for divalent cations on blood platelets. Comparison with the effects of thrombin. Biochim Biophys Acta. 1974 Nov 4;372(1):109–121. doi: 10.1016/0304-4165(74)90077-4. [DOI] [PubMed] [Google Scholar]
- Miller J. L., Katz A. J., Feinstein M. B. Plasmin inhibition of thrombin-induced platelet aggregation. Thromb Diath Haemorrh. 1975 Apr 30;33(2):286–309. [PubMed] [Google Scholar]
- Mills D. C., Robb I. A., Roberts G. C. The release of nucleotides, 5-hydroxytryptamine and enzymes from human blood platelets during aggregation. J Physiol. 1968 Apr;195(3):715–729. doi: 10.1113/jphysiol.1968.sp008484. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Brien J. R., Heywood J. B. Effect of aggregating agents and their inhibitors on the mean platelet shape. J Clin Pathol. 1966 Mar;19(2):148–153. doi: 10.1136/jcp.19.2.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Patscheke H., Brossmer R. Platelet-release reaction induced by the lectin concanavalin A. Naturwissenschaften. 1974 Apr;61(4):164–166. doi: 10.1007/BF00602590. [DOI] [PubMed] [Google Scholar]
- Patscheke H., Wörner P. Common activation of aggregation and released reaction of platelets. Thromb Res. 1977 Sep;11(3):391–402. doi: 10.1016/0049-3848(77)90190-6. [DOI] [PubMed] [Google Scholar]
- Schmukler M., Zieve P. D. The effect of concanavalin A on human platelets and their response to thrombin. J Lab Clin Med. 1974 Jun;83(6):887–895. [PubMed] [Google Scholar]
- Skjorten F. Studies on the ultrastructure of pseudopod formation in human blood platelets. I. Effect of temperature, period of incubation, anticoagulants and mechanical forces. Scand J Haematol. 1968;5(6):401–414. [PubMed] [Google Scholar]
- Tollefsen D. M., Feagler J. R., Majerus P. W. The binding of thrombin to the surface of human platelets. J Biol Chem. 1974 Apr 25;249(8):2646–2651. [PubMed] [Google Scholar]
- White J. G., Rao G. H., Gerrard J. M. Effects of the lonophore A23187 on blood platelets I. Influence on aggregation and secretion. Am J Pathol. 1974 Nov;77(2):135–149. [PMC free article] [PubMed] [Google Scholar]