Abstract
To date, frozen storage of YAC libraries have relied on the administration of glycerol to the medium subsequent to cell growth. By adding Hogness Freezing Medium prior to inoculation, cultures can be frozen directly after cell growth, with no adverse effect on the stability of the YAC DNA or on the viability of the cells even after repeated freezing and defrosting. Although a relatively simple modification, the procedure notably improves the handling of YAC libraries and significantly reduces the risk of contamination, especially when dealing with large numbers of clones.
Full Text
The Full Text of this article is available as a PDF (64.1 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Albertsen H. M., Abderrahim H., Cann H. M., Dausset J., Le Paslier D., Cohen D. Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents. Proc Natl Acad Sci U S A. 1990 Jun;87(11):4256–4260. doi: 10.1073/pnas.87.11.4256. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cox R. D., Meier-Ewert S., Ross M., Larin Z., Monaco A. P., Lehrach H. Genome mapping and cloning of mutations using yeast artificial chromosomes. Methods Enzymol. 1993;225:637–653. doi: 10.1016/0076-6879(93)25041-y. [DOI] [PubMed] [Google Scholar]
- Foote S., Vollrath D., Hilton A., Page D. C. The human Y chromosome: overlapping DNA clones spanning the euchromatic region. Science. 1992 Oct 2;258(5079):60–66. doi: 10.1126/science.1359640. [DOI] [PubMed] [Google Scholar]
- Libert F., Lefort A., Okimoto R., Womack J., Georges M. Construction of a bovine genomic library of large yeast artificial chromosome clones. Genomics. 1993 Nov;18(2):270–276. doi: 10.1006/geno.1993.1465. [DOI] [PubMed] [Google Scholar]
- Qin S., Zhang J., Isaacs C. M., Nagafuchi S., Jani Sait S. N., Abel K. J., Higgins M. J., Nowak N. J., Shows T. B. A chromosome 11 YAC library. Genomics. 1993 Jun;16(3):580–585. doi: 10.1006/geno.1993.1233. [DOI] [PubMed] [Google Scholar]
- Rubio J. P., Triglia T., Kemp D. J., de Bruin D., Ravetch J. V., Cowman A. F. A YAC contig map of Plasmodium falciparum chromosome 4: characterization of a DNA amplification between two recently separated isolates. Genomics. 1995 Mar 20;26(2):192–198. doi: 10.1016/0888-7543(95)80200-6. [DOI] [PubMed] [Google Scholar]
- Schmidt R., West J., Love K., Lenehan Z., Lister C., Thompson H., Bouchez D., Dean C. Physical map and organization of Arabidopsis thaliana chromosome 4. Science. 1995 Oct 20;270(5235):480–483. doi: 10.1126/science.270.5235.480. [DOI] [PubMed] [Google Scholar]
- Zehetner G., Lehrach H. The Reference Library System--sharing biological material and experimental data. Nature. 1994 Feb 3;367(6462):489–491. doi: 10.1038/367489a0. [DOI] [PubMed] [Google Scholar]