Abstract
Line broadening of spin label signals is treated in terms of concentration, viscosity, charge and temperature dependencies. Line broadening of spin label signals may be caused either by spin label interactions or by the interaction between a spin label and a second paramagnetic species. Line broadening has been related to collision frequency in the literature and is treated in that way here. Collision frequency is related to diffusion processes in a way that allows information to be obtained about the diffusion environment. Several potential spin label line-broadening agents are compared as to their effectiveness. Small polymer beads with graduated pore sizes are used to show that collisional broadening has a marked dependence on the long-range structure of the diffusion environment. Application of these results to biological diffusion processes is considered.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barnett R. E., Grisham C. M. Spin exchange of spin labeled probes in a natural membrane. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1362–1366. doi: 10.1016/0006-291x(72)90862-5. [DOI] [PubMed] [Google Scholar]
- Devaux P., McConnell H. M. Lateral diffusion in spin-labeled phosphatidylcholine multilayers. J Am Chem Soc. 1972 Jun 28;94(13):4475–4481. doi: 10.1021/ja00768a600. [DOI] [PubMed] [Google Scholar]
- Keith A. D., Snipes W. Viscosity of cellular protoplasm. Science. 1974 Feb 15;183(4125):666–668. doi: 10.1126/science.183.4125.666. [DOI] [PubMed] [Google Scholar]
- Keith A., Bulfield G., Snipes W. Spin-labeled Neurospora mitochondria. Biophys J. 1970 Jul;10(7):618–629. doi: 10.1016/S0006-3495(70)86324-X. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sackmann E., Träuble H. Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. I. Use of spin labels and optical probes as indicators of the phase transition. J Am Chem Soc. 1972 Jun 28;94(13):4482–4491. doi: 10.1021/ja00768a013. [DOI] [PubMed] [Google Scholar]
- Sackmann E., Träuble H. Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. II. Analysis of electron spin resonance spectra of steroid labels incorporated into lipid membranes. J Am Chem Soc. 1972 Jun 28;94(13):4492–4498. doi: 10.1021/ja00768a014. [DOI] [PubMed] [Google Scholar]
- Scandella C. J., Devaux P., McConnell H. M. Rapid lateral diffusion of phospholipids in rabbit sarcoplasmic reticulum. Proc Natl Acad Sci U S A. 1972 Aug;69(8):2056–2060. doi: 10.1073/pnas.69.8.2056. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Träuble H., Sackmann E. Studies of the crystalline-liquid crystalline phase transition of lipid model membranes. 3. Structure of a steroid-lecithin system below and above the lipid-phase transition. J Am Chem Soc. 1972 Jun 28;94(13):4499–4510. doi: 10.1021/ja00768a015. [DOI] [PubMed] [Google Scholar]