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. 1978 May;22(2):191–207. doi: 10.1016/S0006-3495(78)85484-8

Deuterated phospholipids as nonperturbing components for Raman studies of biomembranes.

B P Gaber, P Yager, W L Peticolas
PMCID: PMC1473445  PMID: 580768

Abstract

The deuterated phospholipid, 1,2-dipalmitoyl-d62-phosphatidylcholine is shown by Raman spectroscopic measurements to be useful for obtaining information concerning phospholipid conformation in complex phospholipid and lipidprotein mixtures. The Raman bands of the deuterated phospholipid are assigned, and the sensitivity of these vibrational modes to conformational changes in the bilayer is demonstrated. Deuteration of the alkyl chains reveals the CH vibrations of the head group. A change in these bands is observed at the melting temperature and is assigned to alteration of the glycerol backbone conformation upon melting.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bunow M. R., Levin I. W. Raman spectra and vibrational assignments for deuterated membrane lipids. 1,2-Dipalmitoyl phosphatidylcholine-d9 and -d62. Biochim Biophys Acta. 1977 Nov 24;489(2):191–206. doi: 10.1016/0005-2760(77)90138-2. [DOI] [PubMed] [Google Scholar]
  2. Cadenhead D. A., Kellner B. M., Müller-Landau F. A comparison of a spin-label and a fluorescent cell membrane probe using pure and mixed monomolecular films. Biochim Biophys Acta. 1975 Mar 13;382(2):253–259. doi: 10.1016/0005-2736(75)90183-2. [DOI] [PubMed] [Google Scholar]
  3. Gaber B. P., Peticolas W. L. On the quantitative interpretation of biomembrane structure by Raman spectroscopy. Biochim Biophys Acta. 1977 Mar 1;465(2):260–274. doi: 10.1016/0005-2736(77)90078-5. [DOI] [PubMed] [Google Scholar]
  4. Gaber B. P., Yager P., Peticolas W. L. Interpretation of biomembrane structure by Raman difference spectroscopy. Nature of the endothermic transitions in phosphatidylcholines. Biophys J. 1978 Feb;21(2):161–176. doi: 10.1016/S0006-3495(78)85516-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mendelsohn R., Sunder S., Bernstein H. J. The effect of sonication on the hydrocarbon chain conformation in model membrane systems: a Raman spectroscopic study. Biochim Biophys Acta. 1976 Feb 6;419(3):563–569. doi: 10.1016/0005-2736(76)90270-4. [DOI] [PubMed] [Google Scholar]
  6. Petersen N. O., Kroon P. A., Kainoshio M., Chan S. I. Thermal phase transitions in deuterated lecithin bilayers. Chem Phys Lipids. 1975 Aug;14(4):343–349. doi: 10.1016/0009-3084(75)90071-7. [DOI] [PubMed] [Google Scholar]
  7. Spiker R. C., Levin I. W. Effect of bilayer curvature on vibrational Raman spectroscopic behavior of phospholipid-water assemblies. Biochim Biophys Acta. 1976 Dec 2;455(2):560–575. doi: 10.1016/0005-2736(76)90325-4. [DOI] [PubMed] [Google Scholar]
  8. Sunder S., Mendelsohn R., Bernstein H. J. Raman studies of the C-H and C-D stretching regions in stearic acid and some specifically deuterated derivatives. Chem Phys Lipids. 1976 Nov;17(4):456–465. doi: 10.1016/0009-3084(76)90047-5. [DOI] [PubMed] [Google Scholar]
  9. Yellin N., Levin I. W. Hydrocarbon trans-gauche isomerization in phospholipid bilayer gel assemblies. Biochemistry. 1977 Feb 22;16(4):642–647. doi: 10.1021/bi00623a014. [DOI] [PubMed] [Google Scholar]

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