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. 1986 Apr;66:45–53. doi: 10.1289/ehp.866645

Toxicity of mycotoxins for the rat pulmonary macrophage in vitro.

W G Sorenson, G F Gerberick, D M Lewis, V Castranova
PMCID: PMC1474366  PMID: 2423320

Abstract

The presence of mycotoxins in grains is well documented. Workers in grain handling occupations are commonly exposed to grain dust aerosols. Work in our laboratory has shown that T-2 toxin is highly toxic to rat alveolar macrophages in vitro, causing loss of viability, release of radiolabeled chromium, inhibition of macromolecular synthesis, inhibition of phagocytosis, and inhibition of macrophage activation. Similarly, patulin caused a significant release of radiolabeled chromium, decrease in ATP levels, significant inhibition of protein and RNA synthesis, and inhibition of phagocytosis. The data show that both T-2 toxin and patulin are highly toxic to rat alveolar macrophages in vitro. The data further suggest that the presence of these mycotoxins in airborne respirable dust might present a hazard to exposed workers.

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Selected References

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  1. Adams D. O., Snyderman R. Do macrophages destroy nascent tumors? J Natl Cancer Inst. 1979 Jun;62(6):1341–1345. [PubMed] [Google Scholar]
  2. Ciegler A., Beckwith A. C., Jackson L. K. Teratogenicity of patulin and patulin adducts formed with cysteine. Appl Environ Microbiol. 1976 May;31(5):664–667. doi: 10.1128/aem.31.5.664-667.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DICKENS F., JONES H. E. Carcinogenic activity of a series of reactive lactones and related substances. Br J Cancer. 1961 Mar;15:85–100. doi: 10.1038/bjc.1961.10. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gerberick G. F., Sorenson W. G., Lewis D. M. The effects of T-2 toxin on alveolar macrophage function in vitro. Environ Res. 1984 Feb;33(1):246–260. doi: 10.1016/0013-9351(84)90021-5. [DOI] [PubMed] [Google Scholar]
  5. Gerberick G. F., Sorenson W. G. Toxicity of T-2 toxin, a Fusarium mycotoxin, to alveolar macrophages in vitro. Environ Res. 1983 Dec;32(2):269–285. doi: 10.1016/0013-9351(83)90111-1. [DOI] [PubMed] [Google Scholar]
  6. Gudewicz P. W., Molnar J., Lai M. Z., Beezhold D. W., Siefring G. E., Jr, Credo R. B., Lorand L. Fibronectin-mediated uptake of gelatin-coated latex particles by peritoneal macrophages. J Cell Biol. 1980 Nov;87(2 Pt 1):427–433. doi: 10.1083/jcb.87.2.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hammond M. E., Dvorak H. F. Antigen-induced stimulation of glucosamine incorporation by guinea pig peritoneal macrophages in delayed hypersensitivity. J Exp Med. 1972 Dec 1;136(6):1518–1532. doi: 10.1084/jem.136.6.1518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hatey F., Gaye P. Inhibition of translation in reticulocyte lysate by the mycotoxin patulin. FEBS Lett. 1978 Nov 15;95(2):252–256. doi: 10.1016/0014-5793(78)81005-9. [DOI] [PubMed] [Google Scholar]
  9. Hsu I. C., Smalley E. B., Strong F. M., Ribelin W. E. Identification of T-2 toxin in moldy corn associated with a lethal toxicosis in dairy cattle. Appl Microbiol. 1972 Nov;24(5):684–690. doi: 10.1128/am.24.5.684-690.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lafarge-Frayssinet C., Decloitre F., Mousset S., Martin M., Frayssinet C. Induction of DNA single-strand breaks by T2 toxin, a trichothecene metabolite of fusarium: effect on lymphoid organs and liver. Mutat Res. 1981 Feb;88(2):115–123. doi: 10.1016/0165-1218(81)90010-0. [DOI] [PubMed] [Google Scholar]
  11. Lafarge-Frayssinet C., Lespinats G., Lafont P., Loisillier F., Mousset S., Rosenstein Y., Frayssinet C. Immunosuppressive effects of Fusarium extracts and trichothecenes: blastogenic response of murine splenic and thymic cells to mitogens. Proc Soc Exp Biol Med. 1979 Mar;160(3):302–311. doi: 10.3181/00379727-160-40439. [DOI] [PubMed] [Google Scholar]
  12. Lafont P., Lafarge-Frayssinet C., Lafont J., Bertin G., Frayssinet France Métabolites toxiques de Fusarium, agents de l'aleucémie toxique alimentaire. Ann Microbiol (Paris) 1977 Aug-Sep;128(2):215–220. [PubMed] [Google Scholar]
  13. Lee S., Chu F. S. Radioimmunoassay of T-2 toxin in corn and wheat. J Assoc Off Anal Chem. 1981 Jan;64(1):156–161. [PubMed] [Google Scholar]
  14. Levy M. H., Wheelock E. F. The role of macrophages in defense against neoplastic disease. Adv Cancer Res. 1974;20:131–163. doi: 10.1016/s0065-230x(08)60110-4. [DOI] [PubMed] [Google Scholar]
  15. Lipscomb M. F., Toews G. B., Lyons C. R., Uhr J. W. Antigen presentation by guinea pig alveolar macrophages. J Immunol. 1981 Jan;126(1):286–291. [PubMed] [Google Scholar]
  16. Massaro D., Kelleher K., Massaro G., Yeager H., Jr Alveolar macrophages: depression of protein synthesis during phagocytosis. Am J Physiol. 1970 Jun;218(6):1533–1539. doi: 10.1152/ajplegacy.1970.218.6.1533. [DOI] [PubMed] [Google Scholar]
  17. Morel-Chany E., Burtin P., Trincal G., Frayssinet C. Cytostatic effects of T2 toxin on cultured human neoplastic cells of intestinal origin. Bull Cancer. 1980;67(2):149–154. [PubMed] [Google Scholar]
  18. Moulé Y., Hatey F. Mechanism of the in vitro inhibition of transcription by patulin, a mycotoxin from Byssochlamys nivea. FEBS Lett. 1977 Feb 15;74(1):121–125. doi: 10.1016/0014-5793(77)80767-9. [DOI] [PubMed] [Google Scholar]
  19. North R. J. The concept of the activated macrophage. J Immunol. 1978 Sep;121(3):806–809. [PMC free article] [PubMed] [Google Scholar]
  20. Oldham J. W., Allred L. E., Milo G. E., Kindig O., Capen C. C. The toxicological evaluation of the mycotoxins T-2 and T-2 tetraol using normal human fibroblasts in vitro. Toxicol Appl Pharmacol. 1980 Jan;52(1):159–168. doi: 10.1016/0041-008x(80)90255-0. [DOI] [PubMed] [Google Scholar]
  21. Rennard S. I., Hunninghake G. W., Bitterman P. B., Crystal R. G. Production of fibronectin by the human alveolar macrophage: mechanism for the recruitment of fibroblasts to sites of tissue injury in interstitial lung diseases. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7147–7151. doi: 10.1073/pnas.78.11.7147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rosenstein Y., Lafarge-Frayssinet C., Lespinats G., Loisillier F., Lafont P., Frayssinet C. Immunosuppressive activity of Fusarium toxins. Effects on antibody synthesis and skin grafts of crude extracts, T2-toxin and diacetoxyscirpenol. Immunology. 1979 Jan;36(1):111–117. [PMC free article] [PubMed] [Google Scholar]
  23. SENTEIN P. Altérations du fuseau mitotique et fragmentation des chromosomes par l'action de la patuline sur l'oeuf d'urodèles en segmentation. C R Seances Soc Biol Fil. 1955 Aug-Sep;149(15-18):1621–1622. [PubMed] [Google Scholar]
  24. Sato N., Ueno Y., Enomoto M. Toxicological approaches to the toxic metabolites of Fusaria. VIII. Acute and subacute toxicities of T-2 toxin in cats. Jpn J Pharmacol. 1975 Jun;25(3):263–270. doi: 10.1254/jjp.25.263. [DOI] [PubMed] [Google Scholar]
  25. Schoental R., Joffe A. Z., Yagen B. Cardiovascular lesions and various tumors found in rats given T-2 toxin, a trichothecene metabolite of Fusarium. Cancer Res. 1979 Jun;39(6 Pt 1):2179–2189. [PubMed] [Google Scholar]
  26. Snyderman R., Pike M. C., Fischer D. G., Koren H. S. Biologic and biochemical activities of continuous macrophage cell lines P388D1 and J774.1. J Immunol. 1977 Dec;119(6):2060–2066. [PubMed] [Google Scholar]
  27. Sorenson W. G., Simpson J. P., Peach M. J., 3rd, Thedell T. D., Olenchock S. A. Aflatoxin in respirable corn dust particles. J Toxicol Environ Health. 1981 Mar-Apr;7(3-4):669–672. doi: 10.1080/15287398109530009. [DOI] [PubMed] [Google Scholar]
  28. Sumbu Z. L., Thonart P., Bechet J. Action of patulin on a yeast. Appl Environ Microbiol. 1983 Jan;45(1):110–115. doi: 10.1128/aem.45.1.110-115.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Ueno Y., Matsumoto H., Ishi K., Kukita K. I. Inhibitory effects of mycotoxins on Na+-dependent transport of glycine in rabbit reticulocytes. Biochem Pharmacol. 1976 Sep 15;25(18):2091–2095. doi: 10.1016/0006-2952(76)90435-4. [DOI] [PubMed] [Google Scholar]
  30. Umeda M., Yamamoto T., Saito M. DNA-strand breakage of HeLa cells induced by several mycotoxins. Jpn J Exp Med. 1972 Dec;42(6):527–535. [PubMed] [Google Scholar]
  31. Waters M. D., Gardner D. E., Aranyi C., Coffin D. L. Metal toxicity for rabbit alveolar macrophages in vitro. Environ Res. 1975 Feb;9(1):32–47. doi: 10.1016/0013-9351(75)90047-x. [DOI] [PubMed] [Google Scholar]
  32. Waters M. D., Vaughan T. O., Abernethy D. J., Garland H. R., Cox C. C., Coffin D. L. Toxicity of platinum (IV) salts for cells of pulmonary origin. Environ Health Perspect. 1975 Dec;12:45–56. doi: 10.1289/ehp.751245. [DOI] [PMC free article] [PubMed] [Google Scholar]

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