Abstract
In cats under chloralose anaesthesia the cerebral ventricles were perfused from a cannula in the lateral ventricle. The effluent was collected either from the cisterna or from the aqueduct. When acetylcholine was added to the perfusion fluid and its destruction was prevented by the addition of an anticholinesterase, its recovery in the internal effluent was incomplete and irregular. This could be explained mainly by the devious route the perfusion fluid had to take in order to reach the cisterna and the unavoidable mixture with the cerebrospinal fluid of the subarachnoidal space. When collection was from the aqueduct 90% or more of the acetylcholine added to the perfusion fluid was recovered. In the presence of an anticholinesterase in the perfusion fluid, acetylcholine appeared in the effluent. Neostigmine was more effective than eserine or dyflos. The acetylcholine originated mainly from structures lining the lateral and third ventricle because the amounts in the effluent from the aqueduct were only a little less than those in the cisternal effluent. When injected intravenously, eserine was found to pass more readily into the perfusion fluid than neostigmine, probably because eserine passes the blood-brain barrier more readily than neostigmine. The method provides a quantitative approach for the study of the blood-brain barrier for pharmacologically active substances.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BHATTACHARYA B. K., FELDBERG W. Comparison of the effects of eserine and neostigmine on the leech muscle preparation. Br J Pharmacol Chemother. 1958 Jun;13(2):151–155. doi: 10.1111/j.1476-5381.1958.tb00210.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BRADLEY P. B., ELKES J. A technique for recording the electrical activity of the brain in the conscious animal. Electroencephalogr Clin Neurophysiol. 1953 Aug;5(3):451–456. doi: 10.1016/0013-4694(53)90091-1. [DOI] [PubMed] [Google Scholar]
- Bülbring E., Burn J. H. Observations bearing on synaptic transmission by acetylcholine in the spinal cord. J Physiol. 1941 Nov 28;100(3):337–368. doi: 10.1113/jphysiol.1941.sp003947. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DESMEDT J. E., LA GRUTTA G. The effect of selective inhibition of pseudocholinesterase on the spontaneous and evoked activity of the cat's cerebral cortex. J Physiol. 1957 Apr 3;136(1):20–40. doi: 10.1113/jphysiol.1957.sp005741. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ECCLES J. C., ECCLES D. M., FATT P. Pharmacological investigations on a central synapse operated by acetylcholine. J Physiol. 1956 Jan 27;131(1):154–169. doi: 10.1113/jphysiol.1956.sp005452. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ECCLES J. C., FATT P., KOKETSU K. Cholinergic and inhibitory synapses in a pathway from motor-axon collaterals to motoneurones. J Physiol. 1954 Dec 10;126(3):524–562. doi: 10.1113/jphysiol.1954.sp005226. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ERDMANN W. D., KEMPE H. D., LUHNING W. Uber die Wirkung von Esteraseblockern (E605, Eserin und Prostigmin) auf das Atemzentrum von Katze und Hund. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1955;225(4):359–368. [PubMed] [Google Scholar]
- Feldberg W., Schriever H. The acetylcholine content of the cerebro-spinal fluid of dogs. J Physiol. 1936 Mar 9;86(3):277–284. doi: 10.1113/jphysiol.1936.sp003362. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HORNYKIEWICZ O., KOBINGER W. Uber den Einfluss von Eserin, Tetraäthylpyrophosphat (TEPP) und Neostigmin auf den Blutdruck und die pressorischen Carotissinusreflexe der Ratte. Naunyn Schmiedebergs Arch Exp Pathol Pharmakol. 1956;228(5):493–500. [PubMed] [Google Scholar]
- Lindberg O., Ernster L. The turnover of radioactive phosphate injected into the subarachnoid space of the brain of the rat. Biochem J. 1950 Jan;46(1):43–47. doi: 10.1042/bj0460043. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RODRIGUEZ L. A. Experiments on the histologic locus of the hemato-encephalic barrier. J Comp Neurol. 1955 Feb;102(1):27–45. doi: 10.1002/cne.901020103. [DOI] [PubMed] [Google Scholar]
- SACKS J., CULBRETH G. G. Phosphate transport and turnover in the brain. Am J Physiol. 1951 Apr 1;165(1):251–256. doi: 10.1152/ajplegacy.1951.165.1.251. [DOI] [PubMed] [Google Scholar]