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. 1961 Oct;17(2):245–260. doi: 10.1111/j.1476-5381.1961.tb01285.x

Effect of guanethidine in revealing cholinergic sympathetic fibres

M D Day, M J Rand
PMCID: PMC1482045  PMID: 13884063

Abstract

Guanethidine abolished the inhibitory response of segments of rabbit intestine to stimulation of the sympathetic nerves which accompany the mesenteric arteries. In the majority of experiments a motor response of the intestinal segment was then revealed; it was more readily observed in intestinal segments from young than from adult rabbits. The motor response of the intestine to sympathetic stimulation after guanethidine was blocked by atropine. It was not blocked by hexamethonium and was present in rabbits in which the vagal innervation to the small intestine had been sectioned. In the cat isolated atria, guanethidine blocked the accelerator response to sympathetic nerve stimulation and revealed a response resembling that to stimulation of the vagus. It was concluded that guanethidine blocked the release of noradrenaline and thus revealed the response to the direct action of acetylcholine released from cholinergic sympathetic nerves.

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Selected References

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  1. BEIN H. J. Zur Pharmakologie des Reserpin, eines neuen Alkaloids aus Rauwolfia serpentina Benth. Experientia. 1953 Mar 15;9(3):107–110. doi: 10.1007/BF02178342. [DOI] [PubMed] [Google Scholar]
  2. BOURA A. L., GREEN A. F. The actions of bretylium: adrenergic neurone blocking and other effects. Br J Pharmacol Chemother. 1959 Dec;14:536–548. doi: 10.1111/j.1476-5381.1959.tb00961.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. BURN J. H. A new view of adrenergic nerve fibres, explaining the action of reserpine, bretylium, and guanethidine. Br Med J. 1961 Jun 10;1(5240):1623–1627. doi: 10.1136/bmj.1.5240.1623. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. BURN J. H., LEACH E. H., RAND M. J., THOMPSON J. W. Peripheral effects of nicotine and acetylcholine resembling those of sympathetic stimulation. J Physiol. 1959 Oct;148:332–352. doi: 10.1113/jphysiol.1959.sp006291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BURN J. H., RAND M. J. Excitatory action of the vagus in the isolated atria in relation to adrenaline. J Physiol. 1958 Jun 18;142(1):173–186. doi: 10.1113/jphysiol.1958.sp006007. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BURN J. H., RAND M. J. New observations on the sympathetic postganglionic mechanism. Am J Med. 1960 Dec;29:1002–1007. doi: 10.1016/0002-9343(60)90080-2. [DOI] [PubMed] [Google Scholar]
  7. BURN J. H., RAND M. J. Noradrenaline in artery walls and its dispersal by reserpine. Br Med J. 1958 Apr 19;1(5076):903–908. doi: 10.1136/bmj.1.5076.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. BURN J. H., RAND M. J. Sympathetic postganglionic cholinergic fibres. Br J Pharmacol Chemother. 1960 Mar;15:56–66. doi: 10.1111/j.1476-5381.1960.tb01210.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. BURN J. H., RAND M. J. The action of sympathomimetic amines in animals treated with reserpine. J Physiol. 1958 Dec 4;144(2):314–336. doi: 10.1113/jphysiol.1958.sp006104. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. BURN J. H., RAND M. J. The cause of the supersensitivity of smooth muscle to noradrenaline after sympathetic degeneration. J Physiol. 1959 Jun 23;147(1):135–143. doi: 10.1113/jphysiol.1959.sp006228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. CASS R., KUNTZMAN R., BRODIE B. B. Norepinephrine depletion as a possible mechanism of action of guanethidine (SU 5864), a new hypotensive agent. Proc Soc Exp Biol Med. 1960 Apr;103:871–872. doi: 10.3181/00379727-103-25702. [DOI] [PubMed] [Google Scholar]
  12. CHANG V., RAND M. J. Transmission failure in sympathetic nerves produced by hemicholinium. Br J Pharmacol Chemother. 1960 Dec;15:588–600. doi: 10.1111/j.1476-5381.1960.tb00287.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Finkleman B. On the nature of inhibition in the intestine. J Physiol. 1930 Sep 18;70(2):145–157. doi: 10.1113/jphysiol.1930.sp002683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. GARRY R. C., GILLESPIE J. S. The responses of the musculature of the colon of the rabbit to stimulation, in vitro, of the parasympathetic and of the sympathetic outflows. J Physiol. 1955 Jun 28;128(3):557–576. doi: 10.1113/jphysiol.1955.sp005325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. GILLESPIE J. S., MACKENNA B. R. The inhibitory action of the sympathetic nerves on the smooth muscle of the rabbit gut, its reversal by reserpine and restoration by catechol amines and by DOPA. J Physiol. 1961 Apr;156:17–34. doi: 10.1113/jphysiol.1961.sp006655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MAXWELL R. A., PLUMMER A. J., SCHNEIDER F., POVALSKI H., DANIEL A. I. Pharmacology of [2-(octahydro-1-azocinyl)-ethyl]-guanidine sulfate (Su-5864). J Pharmacol Exp Ther. 1960 Jan;128:22–29. [PubMed] [Google Scholar]
  17. MCCUBBIN J. W., KANEKO Y., PAGE I. H. The peripheral cardiovascular actions of guanethidine in dogs. J Pharmacol Exp Ther. 1961 Mar;131:346–354. [PubMed] [Google Scholar]
  18. MCEWEN L. M. The effect on the isolated rabbit heart of vagal stimulation and its modification by cocaine, hexamethonium and ouabain. J Physiol. 1956 Mar 28;131(3):678–689. doi: 10.1113/jphysiol.1956.sp005493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MUSCHOLL E., VOGT M. The action of reserpine on the peripheral sympathetic system. J Physiol. 1958 Apr 3;141(1):132–155. doi: 10.1113/jphysiol.1958.sp005961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PAGE I. H., DUSTAN H. P. A new, potent antihypertensive drug: preliminary study of [2-(octahydro-1-azocinyl)-ethyl]-guanidine sulfate (guanethidine). J Am Med Assoc. 1959 Jul 11;170(11):1265–1271. doi: 10.1001/jama.1959.03010110013003. [DOI] [PubMed] [Google Scholar]
  21. PATON W. D. The action of morphine and related substances on contraction and on acetylcholine output of coaxially stimulated guinea-pig ileum. Br J Pharmacol Chemother. 1957 Mar;12(1):119–127. doi: 10.1111/j.1476-5381.1957.tb01373.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. TRENDELENBURG U., GRAVENSTEIN J. S. Effect of reserpine pretreatment on stimulation of the accelerans nerve of the dog. Science. 1958 Oct 17;128(3329):901–903. doi: 10.1126/science.128.3329.901-a. [DOI] [PubMed] [Google Scholar]
  23. WEST G. B., SHEPHERD D. M., HUNTER R. B. Adrenaline and noradrenaline concentrations in adrenal glands at different ages and in some diseases. Lancet. 1951 Nov 24;2(6691):966–969. doi: 10.1016/s0140-6736(51)91928-9. [DOI] [PubMed] [Google Scholar]

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