Abstract
The distribution of 5-hydroxytryptamine, adenosinetriphosphate, and succinic dehydrogenase in sucrose homogenates of the dog's small intestine has been studied. The adenosinetriphosphate was present in two different layers which could be separated by density gradient centrifugation. The upper layer contained also much succinic dehydrogenase, but no amine; it is probably composed of mitochondria. The lower layer contained not only adenosinetriphosphate but also the major portion of the particle-held 5-hydroxytryptamine. The mean molar ratio, amine: adenosinetriphosphate, in the lower layer was 2.6. The experiments suggest that adenosinetriphosphate in the intestine is of importance in the storage of 5-hydroxytryptamine, resembling the function of adenosinetriphosphate in the storage of the catechol amines of the adrenal medulla.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAKER R. V. Observations on the localization of 5-hydroxytryptamine. J Physiol. 1958 Aug 6;142(3):563–570. doi: 10.1113/jphysiol.1958.sp006036. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EADE N. R. The distribution of the catechol amines in homogenates of the bovine adrenal medulla. J Physiol. 1958 Apr 3;141(1):183–192. doi: 10.1113/jphysiol.1958.sp005966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HOLTON P. The liberation of adenosine triphosphate on antidromic stimulation of sensory nerves. J Physiol. 1959 Mar 12;145(3):494–504. doi: 10.1113/jphysiol.1959.sp006157. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KUFF E. L., SCHNEIDER W. C. Intracellular distribution of enzymes. XII. Biochemical heterogeneity of mitochondria. J Biol Chem. 1954 Feb;206(2):677–685. [PubMed] [Google Scholar]
- UDENFRIEND S., WEISSBACH H., CLARK C. T. The estimation of 5-hydroxytryptamine (serotonin) in biological tissues. J Biol Chem. 1955 Jul;215(1):337–344. [PubMed] [Google Scholar]