Abstract
The mechanism of the inhibitory effect of gluten fractions on the peristaltic reflex was studied using, first, a filtrate of the autoclaved peptic-tryptic digest of gluten (fraction III A.F.) and, second, the ultrafiltrate of an aqueous extract of gluten (fraction G.U.F.). The fractions did not act as antagonists to naturally-occurring agents such as acetylcholine or substance P, but they did depress the twitch response of the isolated guinea-pig jejunum preparation stimulated coaxially. The output of acetylcholine from cholinergic nerve endings in gut was decreased during rest as well as during electrical stimulation. In large amounts, the gluten fractions depressed acetylcholine synthesis in vitro. It was concluded that the mechanism of inhibition of the peristaltic reflex by gluten fractions was chiefly by decreasing acetylcholine release.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BULBRING E., CREMA A. The action of 5-hydroxytryptamine, 5-hydroxytryptophan and reserpine on intestinal peristalsis in anaesthetized guinea-pigs. J Physiol. 1959 Apr 23;146(1):29–53. doi: 10.1113/jphysiol.1959.sp006176. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feldberg W., Lin R. C. Synthesis of acetylcholine in the wall of the digestive tract. J Physiol. 1950 Apr 15;111(1-2):96–118. doi: 10.1113/jphysiol.1950.sp004467. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingelfinger F. J., Moss R. E. THE MOTILITY OF THE SMALL INTESTINE IN SPRUE. J Clin Invest. 1943 May;22(3):345–352. doi: 10.1172/JCI101403. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PATON W. D. The action of morphine and related substances on contraction and on acetylcholine output of coaxially stimulated guinea-pig ileum. Br J Pharmacol Chemother. 1957 Mar;12(1):119–127. doi: 10.1111/j.1476-5381.1957.tb01373.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHAUMANN W. Inhibition by morphine of the release of acetylcholine from the intestine of the guinea-pig. Br J Pharmacol Chemother. 1957 Mar;12(1):115–118. doi: 10.1111/j.1476-5381.1957.tb01372.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHNEIDER R., BISHOP H., SHAW B. The inhibition of the peristaltic reflex by substances from protein sources. Br J Pharmacol Chemother. 1960 Jun;15:219–223. doi: 10.1111/j.1476-5381.1960.tb01234.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VANE J. R. A sensitive method for the assay of 5-hydroxytryptamine. Br J Pharmacol Chemother. 1957 Sep;12(3):344–349. doi: 10.1111/j.1476-5381.1957.tb00146.x. [DOI] [PMC free article] [PubMed] [Google Scholar]