Skip to main content
Environmental Health Perspectives logoLink to Environmental Health Perspectives
. 1992 Jul;97:153–157. doi: 10.1289/ehp.9297153

Phagolysosomal pH and dissolution of cobalt oxide particles by alveolar macrophages.

M Lundborg 1, R Falk 1, A Johansson 1, W Kreyling 1, P Camner 1
PMCID: PMC1519542  PMID: 1396451

Abstract

We studied phagolysosomal pH in rabbit alveolar macrophages (AM) incubated with 0-15 microM chloroquine. There was a dose-related increase in pH with chloroquine concentration. Electron microscopy showed that chloroquine increased lysosomal size. In a second experiment we studied dissolution of radiolabeled cobalt oxide particles by rabbit AM, phagolysosomal pH, and lysosomal size. The cells were incubated for 2 days with 0, 2.5, and 10 microM chloroquine. Size and pH increased with chloroquine concentration. Dissolution of cobalt particles by the AM did not clearly change with pH. In a third experiment, dissolution of cobalt oxide particles in 0.1 M acetate buffer in saline with pH 4.0, 5.0, and 6.0 was studied. At the same pH, dissolution in acetate buffer was faster than in the AM, and the dissolution appeared to decrease faster with increasing pH than in the AM. A simple model for dissolution of a particle in a phagolysosome was proposed. This model predicts the types of differences in dissolution between AM and buffered saline.

Full text

PDF
155

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. André S., Métivier H., Lantenois G., Boyer M., Nolibé D., Masse R. Beryllium metal solubility in the lung, comparison of metal and hot-pressed forms by in vivo and in vitro dissolution bioassays. Hum Toxicol. 1987 May;6(3):233–240. doi: 10.1177/096032718700600311. [DOI] [PubMed] [Google Scholar]
  2. Bailey M. R., Fry F. A., James A. C. The long-term clearance kinetics of insoluble particles from the human lung. Ann Occup Hyg. 1982;26(1-4):273–290. [PubMed] [Google Scholar]
  3. Bohning D. E., Atkins H. L., Cohn S. H. Long-term particle clearance in man: normal and impaired. Ann Occup Hyg. 1982;26(1-4):259–271. [PubMed] [Google Scholar]
  4. Geisow M. J., D'Arcy Hart P., Young M. R. Temporal changes of lysosome and phagosome pH during phagolysosome formation in macrophages: studies by fluorescence spectroscopy. J Cell Biol. 1981 Jun;89(3):645–652. doi: 10.1083/jcb.89.3.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jacques Y. V., Bainton D. F. Changes in pH within the phagocytic vacuoles of human neutrophils and monocytes. Lab Invest. 1978 Sep;39(3):179–185. [PubMed] [Google Scholar]
  6. Jensen M. S., Bainton D. F. Temporal changes in pH within the phagocytic vacuole of the polymorphonuclear neutrophilic leukocyte. J Cell Biol. 1973 Feb;56(2):379–388. doi: 10.1083/jcb.56.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johansson A., Robertson B., Curstedt T., Camner P. Alveolar macrophage abnormalities in rabbits exposed to low concentrations of trivalent chromium. Environ Res. 1987 Dec;44(2):279–293. doi: 10.1016/s0013-9351(87)80237-2. [DOI] [PubMed] [Google Scholar]
  8. Lundborg M., Eklund A., Lind B., Camner P. Dissolution of metals by human and rabbit alveolar macrophages. Br J Ind Med. 1985 Sep;42(9):642–645. doi: 10.1136/oem.42.9.642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lundborg M., Lind B., Camner P. Ability of rabbit alveolar macrophages to dissolve metals. Exp Lung Res. 1984;7(1):11–22. doi: 10.3109/01902148409087905. [DOI] [PubMed] [Google Scholar]
  10. Marafante E., Lundborg M., Vahter M., Camner P. Dissolution of two arsenic compounds by rabbit alveolar macrophages in vitro. Fundam Appl Toxicol. 1987 Apr;8(3):382–388. doi: 10.1016/0272-0590(87)90087-x. [DOI] [PubMed] [Google Scholar]
  11. Nilsen A., Nyberg K., Camner P. Intraphagosomal pH in alveolar macrophages after phagocytosis in vivo and in vitro of fluorescein-labeled yeast particles. Exp Lung Res. 1988;14(2):197–207. doi: 10.3109/01902148809115124. [DOI] [PubMed] [Google Scholar]
  12. Nyberg K., Johansson A., Camner P. Intraphagosomal pH in alveolar macrophages studied with fluorescein-labeled amorphous silica particles. Exp Lung Res. 1989;15(1):49–62. doi: 10.3109/01902148909069608. [DOI] [PubMed] [Google Scholar]
  13. Nyberg K., Johansson U., Rundquist I., Camner P. Estimation of pH in individual alveolar macrophage phagolysosomes. Exp Lung Res. 1989 Jul;15(4):499–510. doi: 10.3109/01902148909069614. [DOI] [PubMed] [Google Scholar]
  14. Ohkuma S., Poole B. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327–3331. doi: 10.1073/pnas.75.7.3327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Philipson K., Falk R., Camner P. Long-term lung clearance in humans studied with Teflon particles labeled with chromium-51. Exp Lung Res. 1985;9(1-2):31–42. doi: 10.3109/01902148509061526. [DOI] [PubMed] [Google Scholar]

Articles from Environmental Health Perspectives are provided here courtesy of National Institute of Environmental Health Sciences

RESOURCES