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. 1995 Jun;100(3):419–424. doi: 10.1111/j.1365-2249.1995.tb03716.x

HIV-specific lymphoproliferative responses in asymptomatic HIV-infected individuals.

O Pontesilli 1, M Carlesimo 1, A R Varani 1, R Ferrara 1, E C Guerra 1, M L Bernardi 1, G Ricci 1, A M Mazzone 1, G D'Offizi 1, F Aiuti 1
PMCID: PMC1534461  PMID: 7774051

Abstract

In vitro lymphoproliferative responses to HIV-1 recombinant antigens (gp160, p24, and Rev protein) were studied in 83 patients with asymptomatic HIV-1 infection (CDC groups II and III) and circulating CD4 lymphocyte numbers > 400/mm3. Significant response to at least one of the three antigens was detected in 52.4% of the subjects, but the responses were weak, and concordance of the response to the three antigens was rare, the frequency of individuals responding to each antigen not exceeding 22.4%. Increasing frequencies of response were observed when recall antigens (tetanus toxoid and Candida albicans glycomannoprotein) (65.5%) and anti-CD3 MoAb (76.6%) were used as stimuli. Although a significant association between lymphocyte response to p24, but not gp160, and steadiness of CD4 lymphocyte numbers before the assay was observed, no predictive value for lack of CD4 cell decrease was confirmed for either antigen, and fluctuation of the responses to HIV antigens was seen during subsequent follow up. The panel of T cell assays used could be regarded as appropriate for monitoring both HIV-specific responses and T lymphocyte function during immunotherapy with soluble HIV antigens.

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Selected References

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  1. Bogger-Goren S., Bernstein J. M., Gershon A. A., Ogra P. L. Mucosal cell-mediated immunity to varicella zoster virus: role in protection against disease. J Pediatr. 1984 Aug;105(2):195–199. doi: 10.1016/s0022-3476(84)80112-2. [DOI] [PubMed] [Google Scholar]
  2. Clerici M., Berzofsky J. A., Shearer G. M., Tacket C. O. Exposure to human immunodeficiency virus (HIV) type I indicated by HIV-specific T helper cell responses before detection of infection by polymerase chain reaction and serum antibodies [corrected]. J Infect Dis. 1991 Jul;164(1):178–182. doi: 10.1093/infdis/164.1.178. [DOI] [PubMed] [Google Scholar]
  3. Clerici M., Giorgi J. V., Chou C. C., Gudeman V. K., Zack J. A., Gupta P., Ho H. N., Nishanian P. G., Berzofsky J. A., Shearer G. M. Cell-mediated immune response to human immunodeficiency virus (HIV) type 1 in seronegative homosexual men with recent sexual exposure to HIV-1. J Infect Dis. 1992 Jun;165(6):1012–1019. doi: 10.1093/infdis/165.6.1012. [DOI] [PubMed] [Google Scholar]
  4. Clerici M., Levin J. M., Kessler H. A., Harris A., Berzofsky J. A., Landay A. L., Shearer G. M. HIV-specific T-helper activity in seronegative health care workers exposed to contaminated blood. JAMA. 1994 Jan 5;271(1):42–46. [PubMed] [Google Scholar]
  5. Clerici M., Lucey D. R., Berzofsky J. A., Pinto L. A., Wynn T. A., Blatt S. P., Dolan M. J., Hendrix C. W., Wolf S. F., Shearer G. M. Restoration of HIV-specific cell-mediated immune responses by interleukin-12 in vitro. Science. 1993 Dec 10;262(5140):1721–1724. doi: 10.1126/science.7903123. [DOI] [PubMed] [Google Scholar]
  6. Clerici M., Stocks N. I., Zajac R. A., Boswell R. N., Bernstein D. C., Mann D. L., Shearer G. M., Berzofsky J. A. Interleukin-2 production used to detect antigenic peptide recognition by T-helper lymphocytes from asymptomatic HIV-seropositive individuals. Nature. 1989 Jun 1;339(6223):383–385. doi: 10.1038/339383a0. [DOI] [PubMed] [Google Scholar]
  7. Clerici M., Stocks N. I., Zajac R. A., Boswell R. N., Lucey D. R., Via C. S., Shearer G. M. Detection of three distinct patterns of T helper cell dysfunction in asymptomatic, human immunodeficiency virus-seropositive patients. Independence of CD4+ cell numbers and clinical staging. J Clin Invest. 1989 Dec;84(6):1892–1899. doi: 10.1172/JCI114376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Geretti A. M., Van Baalen C. A., Borleffs J. C., Van Els C. A., Osterhaus A. D. Kinetics and specificities of the T helper-cell response to gp120 in the asymptomatic stage of HIV-1 infection. Scand J Immunol. 1994 Apr;39(4):355–362. doi: 10.1111/j.1365-3083.1994.tb03386.x. [DOI] [PubMed] [Google Scholar]
  9. Kelker H. C., Seidlin M., Vogler M., Valentine F. T. Lymphocytes from some long-term seronegative heterosexual partners of HIV-infected individuals proliferate in response to HIV antigens. AIDS Res Hum Retroviruses. 1992 Aug;8(8):1355–1359. doi: 10.1089/aid.1992.8.1355. [DOI] [PubMed] [Google Scholar]
  10. Kovacs J. A., Vasudevachari M. B., Easter M., Davey R. T., Falloon J., Polis M. A., Metcalf J. A., Salzman N., Baseler M., Smith G. E. Induction of humoral and cell-mediated anti-human immunodeficiency virus (HIV) responses in HIV sero-negative volunteers by immunization with recombinant gp160. J Clin Invest. 1993 Aug;92(2):919–928. doi: 10.1172/JCI116667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krowka J. F., Stites D. P., Jain S., Steimer K. S., George-Nascimento C., Gyenes A., Barr P. J., Hollander H., Moss A. R., Homsy J. M. Lymphocyte proliferative responses to human immunodeficiency virus antigens in vitro. J Clin Invest. 1989 Apr;83(4):1198–1203. doi: 10.1172/JCI114001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Manca F., Habeshaw J. A., Dalgleish A. G. HIV envelope glycoprotein, antigen specific T-cell responses, and soluble CD4. Lancet. 1990 Apr 7;335(8693):811–815. doi: 10.1016/0140-6736(90)90935-x. [DOI] [PubMed] [Google Scholar]
  13. Pantaleo G., Graziosi C., Fauci A. S. New concepts in the immunopathogenesis of human immunodeficiency virus infection. N Engl J Med. 1993 Feb 4;328(5):327–335. doi: 10.1056/NEJM199302043280508. [DOI] [PubMed] [Google Scholar]
  14. Pontesilli O., Carlesimo M., Varani A. R., Ferrara R., D'Offizi G., Aiuti F. In vitro lymphocyte proliferative response to HIV-1 p24 is associated with a lack of CD4+ cell decline. AIDS Res Hum Retroviruses. 1994 Feb;10(2):113–114. doi: 10.1089/aid.1994.10.113. [DOI] [PubMed] [Google Scholar]
  15. Quinti I., Palma C., Guerra E. C., Gomez M. J., Mezzaroma I., Aiuti F., Cassone A. Proliferative and cytotoxic responses to mannoproteins of Candida albicans by peripheral blood lymphocytes of HIV-infected subjects. Clin Exp Immunol. 1991 Sep;85(3):485–492. doi: 10.1111/j.1365-2249.1991.tb05754.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Reddy M. M., Grieco M. H. Cell-mediated immunity to recombinant human immunodeficiency virus (HIV) antigens in HIV-infected populations. J Infect Dis. 1989 Jan;159(1):120–122. doi: 10.1093/infdis/159.1.120. [DOI] [PubMed] [Google Scholar]
  17. Redfield R. R., Birx D. L., Ketter N., Tramont E., Polonis V., Davis C., Brundage J. F., Smith G., Johnson S., Fowler A. A phase I evaluation of the safety and immunogenicity of vaccination with recombinant gp160 in patients with early human immunodeficiency virus infection. Military Medical Consortium for Applied Retroviral Research. N Engl J Med. 1991 Jun 13;324(24):1677–1684. doi: 10.1056/NEJM199106133242401. [DOI] [PubMed] [Google Scholar]
  18. Sattentau Q. J., Dalgleish A. G., Weiss R. A., Beverley P. C. Epitopes of the CD4 antigen and HIV infection. Science. 1986 Nov 28;234(4780):1120–1123. doi: 10.1126/science.2430333. [DOI] [PubMed] [Google Scholar]
  19. Schellekens P. T., Roos M. T., De Wolf F., Lange J. M., Miedema F. Low T-cell responsiveness to activation via CD3/TCR is a prognostic marker for acquired immunodeficiency syndrome (AIDS) in human immunodeficiency virus-1 (HIV-1)-infected men. J Clin Immunol. 1990 Mar;10(2):121–127. doi: 10.1007/BF00918194. [DOI] [PubMed] [Google Scholar]
  20. Schrier R. D., Gnann J. W., Jr, Landes R., Lockshin C., Richman D., McCutchan A., Kennedy C., Oldstone M. B., Nelson J. A. T cell recognition of HIV synthetic peptides in a natural infection. J Immunol. 1989 Feb 15;142(4):1166–1176. [PubMed] [Google Scholar]
  21. Tacket C. O., Baqar S., Munoz C., Murphy J. R. Lymphoproliferative responses to mitogens and HIV-1 envelope glycoprotein among volunteers vaccinated with recombinant gp160. AIDS Res Hum Retroviruses. 1990 Apr;6(4):535–542. doi: 10.1089/aid.1990.6.535. [DOI] [PubMed] [Google Scholar]
  22. Wahren B., Morfeldt-Månsson L., Biberfeld G., Moberg L., Ljungman P., Nordlund S., Bredberg-Rådén U., Werner A., Löwer J., Kurth R. Impaired specific cellular response to HTLV-III before other immune defects in patients with HTLV-III infection. N Engl J Med. 1986 Aug 7;315(6):393–394. [PubMed] [Google Scholar]
  23. Wahren B., Morfeldt-Månsson L., Biberfeld G., Moberg L., Sönnerborg A., Ljungman P., Werner A., Kurth R., Gallo R., Bolognesi D. Characteristics of the specific cell-mediated immune response in human immunodeficiency virus infection. J Virol. 1987 Jun;61(6):2017–2023. doi: 10.1128/jvi.61.6.2017-2023.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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