Abstract
Monoclonal IgM autoantibodies have previously been generated from a patient with lepromatous leprosy. Polyclonal anti-idiotypes raised against two of these monoclonal antibodies (8E7 and TH9) were used in an immunoassay to detect the presence of idiotype in human serum. The anti-idiotypes recognize different but overlapping sets of idiotypic determinants, some of which are present on antibodies which bind to Mycobacterium leprae. Sera were tested from 16 individuals with leprosy, 45 with systemic lupus erythematosus, 20 with Lyme disease, and 80 healthy subjects. Positive sera were detected in all groups (seven, two, three, and four, respectively). In most cases the serum bound to both anti-idiotypes, the idiotype being present in the IgM and/or IgG fraction. Levels of the two idiotypes varied independently of total serum IgG concentration and, in serial samples from one patient, independently of each other. The results indicate that 8E7 and TH9 may be representative of serum antibodies which are commonly expressed in leprosy, but may also be expressed in other diseases and in health; and they suggest that such serum antibodies are encoded by a widely shared set of variable region genes.
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Selected References
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- Duggan D. B., Mackworth-Young C., Kari-Lefvert A., Andre-Schwartz J., Mudd D., McAdam K. P., Schwartz R. S. Polyspecificity of human monoclonal antibodies reactive with Mycobacterium leprae, mitochondria, ssDNA, cytoskeletal proteins, and the acetylcholine receptor. Clin Immunol Immunopathol. 1988 Dec;49(3):327–340. doi: 10.1016/0090-1229(88)90123-7. [DOI] [PubMed] [Google Scholar]
- Gavalchin J., Datta S. K. The NZB X SWR model of lupus nephritis. II. Autoantibodies deposited in renal lesions show a distinctive and restricted idiotypic diversity. J Immunol. 1987 Jan 1;138(1):138–148. [PubMed] [Google Scholar]
- Isenberg D. A., Collins C. Detection of cross-reactive anti-DNA antibody idiotypes on renal tissue-bound immunoglobulins from lupus patients. J Clin Invest. 1985 Jul;76(1):287–294. doi: 10.1172/JCI111959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Isenberg D. A., Shoenfeld Y., Madaio M. P., Rauch J., Reichlin M., Stollar B. D., Schwartz R. S. Anti-DNA antibody idiotypes in systemic lupus erythematosus. Lancet. 1984 Aug 25;2(8400):417–422. doi: 10.1016/s0140-6736(84)92904-0. [DOI] [PubMed] [Google Scholar]
- Isenberg D. A., Shoenfeld Y., Walport M., Mackworth-Young C., Dudeney C., Todd-Pokropek A., Brill S., Weinberger A., Pinkas J. Detection of cross-reactive anti-DNA antibody idiotypes in the serum of systemic lupus erythematosus patients and of their relatives. Arthritis Rheum. 1985 Sep;28(9):999–1007. doi: 10.1002/art.1780280907. [DOI] [PubMed] [Google Scholar]
- Mackworth-Young C., Sabbaga J., Schwartz R. S. Idiotypic markers of polyclonal B cell activation. Public idiotypes shared by monoclonal antibodies derived from patients with systemic lupus erythematosus or leprosy. J Clin Invest. 1987 Feb;79(2):572–581. doi: 10.1172/JCI112849. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Steen V. D., Ramsey-Goldman R. Phenothiazine-induced systemic lupus erythematosus with superior vena cava syndrome: case report and review of the literature. Arthritis Rheum. 1988 Jul;31(7):923–926. doi: 10.1002/art.1780310716. [DOI] [PubMed] [Google Scholar]