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Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1994 Sep;97(3):380–385. doi: 10.1111/j.1365-2249.1994.tb06098.x

Early impairment of gut mucosal immunity in HIV-1-infected children.

A Quesnel 1, P Moja 1, S Blanche 1, C Griscelli 1, C Genin 1
PMCID: PMC1534861  PMID: 7915975

Abstract

This study was performed in 27 HIV-1+ children to characterize the IgA hyperglobulinaemia observed in the serum during the course of HIV-1 infection. By contrast with serum IgG, which increased very early, IgA elevation was related to the decrease of CD4+ cell percentage. It was demonstrated that IgA1 subclass increased selectively. Secretory IgA (SIgA) and IgA and IgG activity to gliadin, bovine serum albumin (BSA) and at a lower level to casein could be detected in the serum at the early stages of HIV infection, but SIgA levels and IgA activity to gliadin further increased during the course of immunodeficiency. By contrast, IgA and IgG activity to tetanus toxoid did not change. These data demonstrate that the hyper IgA, closely related to the degree of immunodeficiency, could be due in part to a disturbance of the gut mucosal immune system. Moreover, impaired intestinal immunity seems to appear very early, and to progress during the course of paediatric HIV-1 infection.

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Selected References

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  1. Ballet J. J., Sulcebe G., Couderc L. J., Danon F., Rabian C., Lathrop M., Clauvel J. P., Seligmann M. Impaired anti-pneumococcal antibody response in patients with AIDS-related persistent generalized lymphadenopathy. Clin Exp Immunol. 1987 Jun;68(3):479–487. [PMC free article] [PubMed] [Google Scholar]
  2. Bernstein L. J., Ochs H. D., Wedgwood R. J., Rubinstein A. Defective humoral immunity in pediatric acquired immune deficiency syndrome. J Pediatr. 1985 Sep;107(3):352–357. doi: 10.1016/s0022-3476(85)80505-9. [DOI] [PubMed] [Google Scholar]
  3. Blanche S., Tardieu M., Duliege A., Rouzioux C., Le Deist F., Fukunaga K., Caniglia M., Jacomet C., Messiah A., Griscelli C. Longitudinal study of 94 symptomatic infants with perinatally acquired human immunodeficiency virus infection. Evidence for a bimodal expression of clinical and biological symptoms. Am J Dis Child. 1990 Nov;144(11):1210–1215. doi: 10.1001/archpedi.1990.02150350042021. [DOI] [PubMed] [Google Scholar]
  4. Conley M. E., Delacroix D. L. Intravascular and mucosal immunoglobulin A: two separate but related systems of immune defense? Ann Intern Med. 1987 Jun;106(6):892–899. doi: 10.7326/0003-4819-106-6-892. [DOI] [PubMed] [Google Scholar]
  5. Delacroix D. L., Elkom K. B., Geubel A. P., Hodgson H. F., Dive C., Vaerman J. P. Changes in size, subclass, and metabolic properties of serum immunoglobulin A in liver diseases and in other diseases with high serum immunoglobulin A. J Clin Invest. 1983 Feb;71(2):358–367. doi: 10.1172/JCI110777. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Engström P. E., Norhagen G., Smith C. I., Söder P. O., Hammarström L. An enzyme-linked immunosorbent assay for the determination of the IgA subclass distribution of antigen-specific antibodies. J Immunol Methods. 1988 Nov 25;115(1):45–53. doi: 10.1016/0022-1759(88)90308-0. [DOI] [PubMed] [Google Scholar]
  7. Fahey J. L., Taylor J. M., Detels R., Hofmann B., Melmed R., Nishanian P., Giorgi J. V. The prognostic value of cellular and serologic markers in infection with human immunodeficiency virus type 1. N Engl J Med. 1990 Jan 18;322(3):166–172. doi: 10.1056/NEJM199001183220305. [DOI] [PubMed] [Google Scholar]
  8. Grodzinsky E., Hed J., Liedén G., Sjögren F., Ström M. Presence of IgA and IgG antigliadin antibodies in healthy adults as measured by micro-ELISA. Effect of various cutoff levels on specificity and sensitivity when diagnosing coeliac disease. Int Arch Allergy Appl Immunol. 1990;92(2):119–123. doi: 10.1159/000235201. [DOI] [PubMed] [Google Scholar]
  9. Guarino A., Tarallo L., Guandalini S., Troncone R., Albano F., Rubino A. Impaired intestinal function in symptomatic HIV infection. J Pediatr Gastroenterol Nutr. 1991 May;12(4):453–458. doi: 10.1097/00005176-199105000-00007. [DOI] [PubMed] [Google Scholar]
  10. Horowitz S., Lorenzsonn V. W., Olsen W. A., Albrecht R., Hong R. Small intestinal disease in T cell deficiency. J Pediatr. 1974 Oct;85(4):457–462. doi: 10.1016/s0022-3476(74)80444-0. [DOI] [PubMed] [Google Scholar]
  11. Kemp M., Husby S., Larsen M. L., Svehag S. E. ELISA analysis of IgA subclass antibodies to dietary antigens. Elevated IgA1 antibodies in children with coeliac disease. Int Arch Allergy Appl Immunol. 1988;87(3):247–253. doi: 10.1159/000234680. [DOI] [PubMed] [Google Scholar]
  12. Kozlowski P. A., Jackson S. Serum IgA subclasses and molecular forms in HIV infection: selective increases in monomer and apparent restriction of the antibody response to IgA1 antibodies mainly directed at env glycoproteins. AIDS Res Hum Retroviruses. 1992 Oct;8(10):1773–1780. doi: 10.1089/aid.1992.8.1773. [DOI] [PubMed] [Google Scholar]
  13. Lems-Van Kan P., Verspaget H. W., Peña A. S. ELISA assay for quantitative measurement of human immunoglobulins IgA, IgG, and IgM in nanograms. J Immunol Methods. 1983 Feb 25;57(1-3):51–57. doi: 10.1016/0022-1759(83)90064-9. [DOI] [PubMed] [Google Scholar]
  14. Mascart-Lemone F., Cadranel S., Van den Broeck J., Dive C., Vaerman J. P., Duchateau J. IgA immune response patterns to gliadin in serum. Int Arch Allergy Appl Immunol. 1988;86(4):412–419. doi: 10.1159/000234627. [DOI] [PubMed] [Google Scholar]
  15. McCaughan G. W., Adams E., Basten A. Human antigen-specific IgA responses in blood and secondary lymphoid tissue: an analysis of help and suppression. J Immunol. 1984 Mar;132(3):1190–1196. [PubMed] [Google Scholar]
  16. McLoughlin L. C., Nord K. S., Joshi V. V., Oleske J. M., Connor E. M. Severe gastrointestinal involvement in children with the acquired immunodeficiency syndrome. J Pediatr Gastroenterol Nutr. 1987 Jul-Aug;6(4):517–524. doi: 10.1097/00005176-198707000-00004. [DOI] [PubMed] [Google Scholar]
  17. Nelson J. A., Wiley C. A., Reynolds-Kohler C., Reese C. E., Margaretten W., Levy J. A. Human immunodeficiency virus detected in bowel epithelium from patients with gastrointestinal symptoms. Lancet. 1988 Feb 6;1(8580):259–262. doi: 10.1016/s0140-6736(88)90348-0. [DOI] [PubMed] [Google Scholar]
  18. Paganelli R., Levinsky R. J., Atherton D. J. Detection of specific antigen within circulating immune complexes: validation of the assay and its application to food antigen-antibody complexes formed in healthy and food-allergic subjects. Clin Exp Immunol. 1981 Oct;46(1):44–53. [PMC free article] [PubMed] [Google Scholar]
  19. Pahwa S. G., Quilop M. T., Lange M., Pahwa R. N., Grieco M. H. Defective B-lymphocyte function in homosexual men in relation to the acquired immunodeficiency syndrome. Ann Intern Med. 1984 Dec;101(6):757–763. doi: 10.7326/0003-4819-101-6-757. [DOI] [PubMed] [Google Scholar]
  20. Pignata C., Budillon G., Monaco G., Nani E., Cuomo R., Parrilli G., Ciccimarra F. Jejunal bacterial overgrowth and intestinal permeability in children with immunodeficiency syndromes. Gut. 1990 Aug;31(8):879–882. doi: 10.1136/gut.31.8.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rieger C. H., Renz H., Vestner R., Brehler C., Horn A. Local and systemic immune responses to food antigens in neonates and infants. Allergy Proc. 1991 Sep-Oct;12(5):309–312. doi: 10.2500/108854191778879197. [DOI] [PubMed] [Google Scholar]
  22. Savilahti E., Viander M., Perkkiö M., Vainio E., Kalimo K., Reunala T. IgA antigliadin antibodies: a marker of mucosal damage in childhood coeliac disease. Lancet. 1983 Feb 12;1(8320):320–322. doi: 10.1016/s0140-6736(83)91627-6. [DOI] [PubMed] [Google Scholar]
  23. Schnittman S. M., Lane H. C., Higgins S. E., Folks T., Fauci A. S. Direct polyclonal activation of human B lymphocytes by the acquired immune deficiency syndrome virus. Science. 1986 Sep 5;233(4768):1084–1086. doi: 10.1126/science.3016902. [DOI] [PubMed] [Google Scholar]
  24. Scott G. B., Buck B. E., Leterman J. G., Bloom F. L., Parks W. P. Acquired immunodeficiency syndrome in infants. N Engl J Med. 1984 Jan 12;310(2):76–81. doi: 10.1056/NEJM198401123100202. [DOI] [PubMed] [Google Scholar]
  25. Scott H., Fausa O., Ek J., Brandtzaeg P. Immune response patterns in coeliac disease. Serum antibodies to dietary antigens measured by an enzyme linked immunosorbent assay (ELISA). Clin Exp Immunol. 1984 Jul;57(1):25–32. [PMC free article] [PubMed] [Google Scholar]
  26. Ullrich R., Zeitz M., Heise W., L'age M., Höffken G., Riecken E. O. Small intestinal structure and function in patients infected with human immunodeficiency virus (HIV): evidence for HIV-induced enteropathy. Ann Intern Med. 1989 Jul 1;111(1):15–21. doi: 10.7326/0003-4819-111-1-15. [DOI] [PubMed] [Google Scholar]
  27. Unsworth D. J., Kieffer M., Holborow E. J., Coombs R. R., Walker-Smith J. A. IgA anti-gliadin antibodies in coeliac disease. Clin Exp Immunol. 1981 Nov;46(2):286–293. [PMC free article] [PubMed] [Google Scholar]
  28. Volta U., Molinaro N., Fratangelo D., Bianchi F. B. IgA subclass antibodies to gliadin in serum and intestinal juice of patients with coeliac disease. Clin Exp Immunol. 1990 May;80(2):192–195. doi: 10.1111/j.1365-2249.1990.tb05232.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yarchoan R., Redfield R. R., Broder S. Mechanisms of B cell activation in patients with acquired immunodeficiency syndrome and related disorders. Contribution of antibody-producing B cells, of Epstein-Barr virus-infected B cells, and of immunoglobulin production induced by human T cell lymphotropic virus, type III/lymphadenopathy-associated virus. J Clin Invest. 1986 Aug;78(2):439–447. doi: 10.1172/JCI112595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Yolken R. H., Li S., Perman J., Viscidi R. Persistent diarrhea and fecal shedding of retroviral nucleic acids in children infected with human immunodeficiency virus. J Infect Dis. 1991 Jul;164(1):61–66. doi: 10.1093/infdis/164.1.64. [DOI] [PubMed] [Google Scholar]
  31. van Elburg R. M., Uil J. J., de Monchy J. G., Heymans H. S. Intestinal permeability in pediatric gastroenterology. Scand J Gastroenterol Suppl. 1992;194:19–24. doi: 10.3109/00365529209096021. [DOI] [PubMed] [Google Scholar]

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