Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1990 Jun;80(3):435–443. doi: 10.1111/j.1365-2249.1990.tb03306.x

Cytokine regulation of human monocyte interleukin-1 (IL-1) production in vitro. Enhancement of IL-1 production by interferon (IFN) gamma, tumour necrosis factor-alpha, IL-2 and IL-1, and inhibition by IFN-alpha.

V A Danis 1, A J Kulesz 1, D S Nelson 1, P M Brooks 1
PMCID: PMC1535198  PMID: 2115419

Abstract

IL-1 production (secreted and cell-associated) was measured in monocyte cultures stimulated by a variety of agents in vitro. Monocytes either adherent to conventional plastic culture plates in serum-free conditions, or in suspension in culture medium containing serum were stimulated to produce IL-1 during culture. In non-adherent, serum-free conditions, monocytes produced very low or undetectable amounts of IL-1 during 20 h of culture. Lipopolysaccharide (LPS) induced equivalent amounts of secreted and cell-associated IL-1, although at very low concentrations more cell-associated IL-1 was produced. IL-1 production in response to LPS could be augmented by crude lymphokine, IFN-gamma, or tumour necrosis factor (TNF) alpha. TNF-alpha preferentially augmented the production of cell-associated IL-1 in LPS-stimulated cultures. TNF-alpha induced a significant amount of IL-1 (mainly cell-associated) directly but could also induce IL-1 secretion when combined with IL-2 or IFN-gamma, or when in the presence of serum. IL-2 acted synergistically with low concentrations of IFN-gamma or IL-1 to induce significant levels of IL-1 production. IFN-alpha did not induce any IL-1 production, but was a potent inhibitor of IL-1 production induced by a variety of stimuli. These results suggest that IL-1 production may be enhanced or reduced by different cytokines at concentrations likely to be found in chronic inflammatory lesions.

Full text

PDF
437

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayne E. K., Rupp E. A., Limjuco G., Chin J., Schmidt J. A. Immunocytochemical detection of interleukin 1 within stimulated human monocytes. J Exp Med. 1986 May 1;163(5):1267–1280. doi: 10.1084/jem.163.5.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Buchan G., Barrett K., Turner M., Chantry D., Maini R. N., Feldmann M. Interleukin-1 and tumour necrosis factor mRNA expression in rheumatoid arthritis: prolonged production of IL-1 alpha. Clin Exp Immunol. 1988 Sep;73(3):449–455. [PMC free article] [PubMed] [Google Scholar]
  3. Chantry D., Winearls C. G., Maini R. N., Feldmann M. Mechanism of immune complex-mediated damage: induction of interleukin 1 by immune complexes and synergy with interferon-gamma and tumor necrosis factor-alpha. Eur J Immunol. 1989 Jan;19(1):189–192. doi: 10.1002/eji.1830190130. [DOI] [PubMed] [Google Scholar]
  4. Dalton B. J., Connor J. R., Johnson W. J. Interleukin-1 induces interleukin-1 alpha and interleukin-1 beta gene expression in synovial fibroblasts and peripheral blood monocytes. Arthritis Rheum. 1989 Mar;32(3):279–287. doi: 10.1002/anr.1780320309. [DOI] [PubMed] [Google Scholar]
  5. Danis V. A., March L. M., Nelson D. S., Brooks P. M. Interleukin-1 secretion by peripheral blood monocytes and synovial macrophages from patients with rheumatoid arthritis. J Rheumatol. 1987 Feb;14(1):33–39. [PubMed] [Google Scholar]
  6. Di Giovine F. S., Nuki G., Duff G. W. Tumour necrosis factor in synovial exudates. Ann Rheum Dis. 1988 Sep;47(9):768–772. doi: 10.1136/ard.47.9.768. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dinarello C. A., Cannon J. G., Wolff S. M., Bernheim H. A., Beutler B., Cerami A., Figari I. S., Palladino M. A., Jr, O'Connor J. V. Tumor necrosis factor (cachectin) is an endogenous pyrogen and induces production of interleukin 1. J Exp Med. 1986 Jun 1;163(6):1433–1450. doi: 10.1084/jem.163.6.1433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dinarello C. A., Ikejima T., Warner S. J., Orencole S. F., Lonnemann G., Cannon J. G., Libby P. Interleukin 1 induces interleukin 1. I. Induction of circulating interleukin 1 in rabbits in vivo and in human mononuclear cells in vitro. J Immunol. 1987 Sep 15;139(6):1902–1910. [PubMed] [Google Scholar]
  9. Fuhlbrigge R. C., Chaplin D. D., Kiely J. M., Unanue E. R. Regulation of interleukin 1 gene expression by adherence and lipopolysaccharide. J Immunol. 1987 Jun 1;138(11):3799–3802. [PubMed] [Google Scholar]
  10. Gerrard T. L., Siegel J. P., Dyer D. R., Zoon K. C. Differential effects of interferon-alpha and interferon-gamma on interleukin 1 secretion by monocytes. J Immunol. 1987 Apr 15;138(8):2535–2540. [PubMed] [Google Scholar]
  11. Hart P. H., Whitty G. A., Piccoli D. S., Hamilton J. A. Control by IFN-gamma and PGE2 of TNF alpha and IL-1 production by human monocytes. Immunology. 1989 Mar;66(3):376–383. [PMC free article] [PubMed] [Google Scholar]
  12. Henderson B., Pettipher E. R., Higgs G. A. Mediators of rheumatoid arthritis. Br Med Bull. 1987 Apr;43(2):415–428. doi: 10.1093/oxfordjournals.bmb.a072191. [DOI] [PubMed] [Google Scholar]
  13. Hertzog P. J., Emery P., Cheetham B. F., Mackay I. R., Linnane A. W. Interferons in rheumatoid arthritis: alterations in production and response related to disease activity. Clin Immunol Immunopathol. 1988 Aug;48(2):192–201. doi: 10.1016/0090-1229(88)90083-9. [DOI] [PubMed] [Google Scholar]
  14. Hofsli E., Lamvik J., Nissen-Meyer J. Evidence that tumour necrosis factor (TNF) is not constitutively present in vivo. The association of TNF with freshly isolated monocytes reflects a rapid in vitro production. Scand J Immunol. 1988 Oct;28(4):435–441. doi: 10.1111/j.1365-3083.1988.tb01473.x. [DOI] [PubMed] [Google Scholar]
  15. Hopkins S. J., Meager A. Cytokines in synovial fluid: II. The presence of tumour necrosis factor and interferon. Clin Exp Immunol. 1988 Jul;73(1):88–92. [PMC free article] [PubMed] [Google Scholar]
  16. Hurme M., Serkkola E. Comparison of interleukin 1 release and interleukin 1 mRNA expression of human monocytes activated by bacterial lipopolysaccharide or synthetic lipid A. Scand J Immunol. 1989 Aug;30(2):259–263. doi: 10.1111/j.1365-3083.1989.tb01209.x. [DOI] [PubMed] [Google Scholar]
  17. Le Moal M. A., Stoeck M., Cavaillon J. M., MacDonald H. O., Truffa-Bachi P. A sensitive, IL-2-independent, assay for IL-1. J Immunol Methods. 1988 Feb 24;107(1):23–30. doi: 10.1016/0022-1759(88)90004-x. [DOI] [PubMed] [Google Scholar]
  18. Lepe-Zuniga J. L., Gery I. Production of intra- and extracellular interleukin-1 (IL-1) by human monocytes. Clin Immunol Immunopathol. 1984 May;31(2):222–230. doi: 10.1016/0090-1229(84)90242-3. [DOI] [PubMed] [Google Scholar]
  19. Manson J. C., Symons J. A., Di Giovine F. S., Poole S., Duff G. W. Autoregulation of interleukin 1 production. Eur J Immunol. 1989 Feb;19(2):261–265. doi: 10.1002/eji.1830190207. [DOI] [PubMed] [Google Scholar]
  20. Mosley B., Urdal D. L., Prickett K. S., Larsen A., Cosman D., Conlon P. J., Gillis S., Dower S. K. The interleukin-1 receptor binds the human interleukin-1 alpha precursor but not the interleukin-1 beta precursor. J Biol Chem. 1987 Mar 5;262(7):2941–2944. [PubMed] [Google Scholar]
  21. Newton R. C. Effect of interferon on the induction of human monocyte secretion of interleukin-1 activity. Immunology. 1985 Nov;56(3):441–449. [PMC free article] [PubMed] [Google Scholar]
  22. Numerof R. P., Aronson F. R., Mier J. W. IL-2 stimulates the production of IL-1 alpha and IL-1 beta by human peripheral blood mononuclear cells. J Immunol. 1988 Dec 15;141(12):4250–4257. [PubMed] [Google Scholar]
  23. Portillo G., Turner M., Chantry D., Feldmann M. Effect of cytokines on HLA-DR and IL-1 production by a monocytic tumour, THP-1. Immunology. 1989 Feb;66(2):170–175. [PMC free article] [PubMed] [Google Scholar]
  24. Ruschen S., Lemm G., Warnatz H. Interleukin-2 secretion by synovial fluid lymphocytes in rheumatoid arthritis. Br J Rheumatol. 1988 Oct;27(5):350–356. doi: 10.1093/rheumatology/27.5.350. [DOI] [PubMed] [Google Scholar]
  25. Seitz M., Napierski I., Augustin R., Hunstein W., Kirchner H. Reduced production of interferon alpha and interferon gamma in leukocyte cultures from patients with active rheumatoid arthritis. Scand J Rheumatol. 1987;16(4):257–262. doi: 10.3109/03009748709102926. [DOI] [PubMed] [Google Scholar]
  26. Tilden A. B., Dunlap N. E. Interleukin-2 augmentation of interleukin-1 and prostaglandin E2 production. J Leukoc Biol. 1989 May;45(5):474–477. doi: 10.1002/jlb.45.5.474. [DOI] [PubMed] [Google Scholar]
  27. Van der Meer J. W., Van de Gevel J. S., Van Hinsbergh V. W., Leijh P. C. The influence of culture conditions and serum lipids on interleukin-1 production by human monocytes. J Immunol Methods. 1988 Apr 6;108(1-2):19–26. doi: 10.1016/0022-1759(88)90397-3. [DOI] [PubMed] [Google Scholar]
  28. Winchurch R. A. Activation of thymocyte responses to interleukin-1 by zinc. Clin Immunol Immunopathol. 1988 May;47(2):174–180. doi: 10.1016/0090-1229(88)90070-0. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES