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. 1990 Apr;80(1):77–82. doi: 10.1111/j.1365-2249.1990.tb06444.x

Parasite antigens recognized by patients with cutaneous leishmaniasis.

C L Jaffe 1, R Shor 1, H Trau 1, J H Passwell 1
PMCID: PMC1535222  PMID: 2323102

Abstract

Humoral and cell-mediated responses to crude and purified parasite antigens were examined in patients with active cutaneous leishmaniasis caused by Leishmania major. The patients had serum antibody titres against parasite lysates ranging from 1/500 to 1/10,000 and recognized multiple components by Western blotting with molecular weights between 5000 and greater than 200,000. Several components, particularly at 5 and 50 kD, were recognized by most of the patients. The lymphoproliferative responses to two pure antigens, promastigote surface protease and lipophosphoglycan, both considered potential candidates for the development of a human vaccine were measured. No response to promastigote surface protease was observed even at 10 micrograms/ml; however, weak proliferation to lipophosphoglycan was consistently present. T cell blots examining peripheral blood lymphocyte proliferation, showed antigen-specific responses to a 72-82 kD component in promastigote lysates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bouvier J., Etges R. J., Bordier C. Identification and purification of membrane and soluble forms of the major surface protein of Leishmania promastigotes. J Biol Chem. 1985 Dec 15;260(29):15504–15509. [PubMed] [Google Scholar]
  2. Champsi J., McMahon-Pratt D. Membrane glycoprotein M-2 protects against Leishmania amazonensis infection. Infect Immun. 1988 Dec;56(12):3272–3279. doi: 10.1128/iai.56.12.3272-3279.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Handman E., Mitchell G. F. Immunization with Leishmania receptor for macrophages protects mice against cutaneous leishmaniasis. Proc Natl Acad Sci U S A. 1985 Sep;82(17):5910–5914. doi: 10.1073/pnas.82.17.5910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Huszar M., Shor R., Trau H., Gazit E., Passwell J. H. The T cell phenotypes in the lesion of patients with cutaneous leishmaniasis. Clin Exp Dermatol. 1987 Mar;12(2):103–107. doi: 10.1111/j.1365-2230.1987.tb01874.x. [DOI] [PubMed] [Google Scholar]
  5. Jaffe C. L., McMahon-Pratt D. Monoclonal antibodies specific for Leishmania tropica. I. Characterization of antigens associated with stage- and species-specific determinants. J Immunol. 1983 Oct;131(4):1987–1993. [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Melby P. C., Sacks D. L. Analysis of the heterogeneity of T-cell responses in human leishmaniasis. Ann Inst Pasteur Immunol. 1987 Sep-Oct;138(5):768–771. doi: 10.1016/s0769-2625(87)80035-1. [DOI] [PubMed] [Google Scholar]
  8. Modlin R. L., Tapia F. J., Bloom B. R., Gallinoto M. E., Castes M., Rondon A. J., Rea T. H., Convit J. In situ characterization of the cellular immune response in American cutaneous leishmaniasis. Clin Exp Immunol. 1985 May;60(2):241–248. [PMC free article] [PubMed] [Google Scholar]
  9. Müller I., Pedrazzini T., Farrell J. P., Louis J. T-cell responses and immunity to experimental infection with leishmania major. Annu Rev Immunol. 1989;7:561–578. doi: 10.1146/annurev.iy.07.040189.003021. [DOI] [PubMed] [Google Scholar]
  10. Nguyen T. V., Mauel J., Etges R. J., Bouvier J., Bordier C. Immunogenic proteins of Leishmania major during mouse infection. Parasite Immunol. 1984 May;6(3):265–273. doi: 10.1111/j.1365-3024.1984.tb00799.x. [DOI] [PubMed] [Google Scholar]
  11. Passwell J. H., Shor R., Shoham J. The enhancing effect of interferon-beta and -gamma on the killing of Leishmania tropica major in human mononuclear phagocytes in vitro. J Immunol. 1986 Apr 15;136(8):3062–3066. [PubMed] [Google Scholar]
  12. Passwell J. H., Shor R., Trau H., Shoham J., Jaffe C. L. Antigen-stimulated lymphokines from patients with cutaneous leishmaniasis induce monocyte killing of Leishmania major intracellular amastigotes. J Immunol. 1987 Dec 15;139(12):4208–4212. [PubMed] [Google Scholar]
  13. Russell D. G., Alexander J. Effective immunization against cutaneous leishmaniasis with defined membrane antigens reconstituted into liposomes. J Immunol. 1988 Feb 15;140(4):1274–1279. [PubMed] [Google Scholar]
  14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Young D. B., Lamb J. R. T lymphocytes respond to solid-phase antigen: a novel approach to the molecular analysis of cellular immunity. Immunology. 1986 Oct;59(2):167–171. [PMC free article] [PubMed] [Google Scholar]

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