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. 1991 Jan;83(1):182–186. doi: 10.1111/j.1365-2249.1991.tb05611.x

Production by cultured human monocytes of mesangial cell proliferation factor(s) differing from interleukin-1 and interleukin-6.

T Morioka 1, I Narita 1, F Shimizu 1, T Oite 1
PMCID: PMC1535484  PMID: 1988227

Abstract

Conditioned media from human peripheral blood leucocytes treated with lipopolysaccharide (LPS) induced a marked increase in the 3H-thymidine incorporation of cultured mesangial cells at low serum concentration (four to six times higher than control). Two sizes (100-70 and 8-12 kD) of monocyte-derived mesangial cell proliferating factors (MDF) were separated by column chromatography. Their peaks were distinct from those of thymocyte proliferating activity. The addition of anti-human interleukin-1 (IL-1) or anti-recombinant human interleukin-6 (IL-6) antibody to the fractionated MDF failed to have any effect on the mitogenic activity toward mesangial cells. The addition of anti-human platelet-derived growth factor (PDGF) antibody to the low molecular weight fraction decreased mesangial cell mitogenic activity (40-60% of control), but addition to the higher fraction did not (80-100% of control). From these data it seems that a large portion of the monocyte-derived mesangial cell growth factor was not comprised of IL-1 or IL-6 but of PDGF-like molecules; and that there is an unknown mesangial cell proliferating factor (or factors) besides IL-1, IL-6 and PDGF.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins R. C., Holdsworth S. R., Glasgow E. F., Matthews F. E. The macrophagen in human rapidly progressive glomerulonephritis. Lancet. 1976 Apr 17;1(7964):830–832. doi: 10.1016/s0140-6736(76)90480-3. [DOI] [PubMed] [Google Scholar]
  2. Horii Y., Muraguchi A., Iwano M., Matsuda T., Hirayama T., Yamada H., Fujii Y., Dohi K., Ishikawa H., Ohmoto Y. Involvement of IL-6 in mesangial proliferative glomerulonephritis. J Immunol. 1989 Dec 15;143(12):3949–3955. [PubMed] [Google Scholar]
  3. Lovett D. H., Ryan J. L., Sterzel R. B. Stimulation of rat mesangial cell proliferation by macrophage interleukin 1. J Immunol. 1983 Dec;131(6):2830–2836. [PubMed] [Google Scholar]
  4. MacCarthy E. P., Hsu A., Ooi Y. M., Ooi B. S. Modulation of mouse mesangial cell proliferation by macrophage products. Immunology. 1985 Dec;56(4):695–699. [PMC free article] [PubMed] [Google Scholar]
  5. Melcion C., Lachman L., Killen P. D., Morel-Maroger L., Striker G. E. Mesangial cells, effect of monocyte products on proliferation and matrix synthesis. Transplant Proc. 1982 Sep;14(3):559–564. [PubMed] [Google Scholar]
  6. Morita T., Oite T., Kihara I., Yamamoto T., Hara M., Naka A., Ohno S. Culture of isolated glomeruli from normal and nephritic rabbits. I. Characterization of outgrowing cells. Acta Pathol Jpn. 1980 Nov;30(6):917–926. doi: 10.1111/j.1440-1827.1980.tb03280.x. [DOI] [PubMed] [Google Scholar]
  7. Oite T., Morita T., Kihara I., Yamamoto T., Suzuki Y., Okada K. Culture of isolated glomeruli from normal and nephritic rabbits. II. Kinetics of outgrowing cells. Acta Pathol Jpn. 1980 Nov;30(6):927–935. doi: 10.1111/j.1440-1827.1980.tb03281.x. [DOI] [PubMed] [Google Scholar]
  8. Raines E. W., Dower S. K., Ross R. Interleukin-1 mitogenic activity for fibroblasts and smooth muscle cells is due to PDGF-AA. Science. 1989 Jan 20;243(4889):393–396. doi: 10.1126/science.2783498. [DOI] [PubMed] [Google Scholar]
  9. Shimokado K., Raines E. W., Madtes D. K., Barrett T. B., Benditt E. P., Ross R. A significant part of macrophage-derived growth factor consists of at least two forms of PDGF. Cell. 1985 Nov;43(1):277–286. doi: 10.1016/0092-8674(85)90033-9. [DOI] [PubMed] [Google Scholar]
  10. Shultz P. J., DiCorleto P. E., Silver B. J., Abboud H. E. Mesangial cells express PDGF mRNAs and proliferate in response to PDGF. Am J Physiol. 1988 Oct;255(4 Pt 2):F674–F684. doi: 10.1152/ajprenal.1988.255.4.F674. [DOI] [PubMed] [Google Scholar]
  11. Singh J. P., Bonin P. D. Purification and biochemical properties of a human monocyte-derived growth factor. Proc Natl Acad Sci U S A. 1988 Sep;85(17):6374–6378. doi: 10.1073/pnas.85.17.6374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sterzel R. B., Pabst R. The temporal relationship between glomerular cell proliferation and monocyte infiltration in experimental glomerulonephritis. Virchows Arch B Cell Pathol Incl Mol Pathol. 1982;38(3):337–350. doi: 10.1007/BF02892829. [DOI] [PubMed] [Google Scholar]
  13. Wagner C. M., Lucas D. O., Nagle R. B. The effect of macrophages on the metabolism of glomerular cells: preliminary studies. J Reticuloendothel Soc. 1983 Feb;33(2):93–107. [PubMed] [Google Scholar]

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