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. 1984 Mar;55(3):561–570.

A new anti-liver-kidney microsome antibody (anti-LKM2) in tienilic acid-induced hepatitis.

J C Homberg, C Andre, N Abuaf
PMCID: PMC1535915  PMID: 6368059

Abstract

The sera of 131 patients with anti-liver-kidney microsome antibodies (anti-LKM) detected between 1973 and 1979 in two different laboratories were re-examined. (1) Eighty-six anti-LKM corresponded to the description given by Rizzetto, Swana & Doniach (1973), with a pattern of fluorescence predominating on the 3rd portion of the proximal tubules (P3). This group comprised 45 cases of idiopathic chronic hepatitis or idiopathic cirrhosis and one case of halothane-induced hepatitis. (2) Forty-five anti-LKM gave a different pattern on male mouse liver and male rat kidney: (a) fluorescence was greater on centrolobular than on periportal hepatocytes; (b) the first and second portions of proximal tubules (P1 and P2) predominated over P3; (c) P1 fluorescence was equally intense as P2 and (d) P3 cells were heterogeneous with one cell out of 20 more positive than the rest. Absorption tests confirmed that the corresponding antigen was also present in the liver microsomal fraction. A retrospective clinical study discovered tienilic acid-induced hepatitis in all cases. We suggest naming this new antibody 'anti-LKM2'.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C., Denman A. M., Barnes R. D. Cooperating and controlling functions of thymus-derived lymphocytes in relation to autoimmunity. Lancet. 1971 Jul 17;2(7716):135–140. doi: 10.1016/s0140-6736(71)92306-3. [DOI] [PubMed] [Google Scholar]
  2. Bousquet O., Saigot T., Bernard P., Gerbal J. L., Sarrazin A. Hépatite secondaire à la prise d'acide tiénilique. Therapie. 1980 Mar-Apr;35(2):205–208. [PubMed] [Google Scholar]
  3. Cox K. O., Keast D. Erythrocyte autoantibodies induced in mice immunized with rat erythrocytes. Immunology. 1973 Sep;25(3):531–539. [PMC free article] [PubMed] [Google Scholar]
  4. Dangoumau J., Begaud B., Boisseau A., Albin H. Méthodes d'identification et d'imputabilité des effets indésirables des médicaments. L'effet indesirable, une réalité fuyante? Therapie. 1980 May-Jun;35(3):287–292. [PubMed] [Google Scholar]
  5. Grob P. J., Müller-Schoop J. W., Häcki M. A., Joller-Jemelka H. I. Drug-induced pseudolupus. Lancet. 1975 Jul 26;2(7926):144–148. doi: 10.1016/s0140-6736(75)90055-0. [DOI] [PubMed] [Google Scholar]
  6. Groussin P., Chavanne D., Renard J. P., Medelsi M., Arbeille-Brassard B., Royere D. Hépatite cytolytique, rôle de l'acide tiénilique. Med Chir Dig. 1979;8(5):463–465. [PubMed] [Google Scholar]
  7. Homberg J. C., Stelly N., Andreis I., Abuaf N., Saadoun F., Andre J. A new antimitochondria antibody (anti-M6) in iproniazid-induced hepatitis. Clin Exp Immunol. 1982 Jan;47(1):93–102. [PMC free article] [PubMed] [Google Scholar]
  8. Maas D., Schubothe H. Ein lupus-erythematodes-ähnliches Syndrom mit antimitochondrialen Antikörpern. Dtsch Med Wochenschr. 1973 Jan 26;98(4):131–139. doi: 10.1055/s-0028-1106763. [DOI] [PubMed] [Google Scholar]
  9. Mechali D., Marche C., Metayer J., Saimot G., Matheron S., Rozenbaum W., Coulaud J. P. Hépatite cytolytique au cours d'un traitement par l'acide tiénilique. Therapie. 1980 Mar-Apr;35(2):197–203. [PubMed] [Google Scholar]
  10. Neuberger J., Mieli-Vergani G., Tredger J. M., Davis M., Williams R. Oxidative metabolism of halothane in the production of altered hepatocyte membrane antigens in acute halothane-induced hepatic necrosis. Gut. 1981 Aug;22(8):669–672. doi: 10.1136/gut.22.8.669. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pariente E. A., André C., Zafrani E. S., Métreau J. M., Dhumeaux D., Berthelot P. Hépatite aiguë, hépatite chronique et cirrhose a l'acide tiénilique. Gastroenterol Clin Biol. 1981 May;5(5):567–571. [PubMed] [Google Scholar]
  12. Poupon R., Homberg J. C., Abuaf N., Petit J., Bodin F., Darnis F. Atteintes hépatiques dues à l'acide tiénilique. Six observations avec présence d'anticorps antireticulum endoplasmique. Nouv Presse Med. 1980 Jun 21;9(27):1881–1884. [PubMed] [Google Scholar]
  13. Rizzetto M., Bianchi F. B., Doniach D. Characterization of the microsomal antigen related to a subclass of active chronic hepatitis. Immunology. 1974 Mar;26(3):589–601. [PMC free article] [PubMed] [Google Scholar]
  14. Rizzetto M., Swana G., Doniach D. Microsomal antibodies in active chronic hepatitis and other disorders. Clin Exp Immunol. 1973 Nov;15(3):331–344. [PMC free article] [PubMed] [Google Scholar]
  15. Schmidt G., Kienle J., Schmidt D. S., Wolf H. Lebererkrankungen mit mikrosomalen Antikörpern Häufigkeit, klinische und immunologische Befunde sowie Verlaufsbeobachtungen. Z Gastroenterol. 1978 Aug;16(8):512–520. [PubMed] [Google Scholar]
  16. Smith M. G., Williams R., Walker G., Rizzetto M., Doniach D. Hepatic disorders associated with liver-kidney microsomal antibodies. Br Med J. 1974 Apr 13;2(5910):80–84. doi: 10.1136/bmj.2.5910.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vergani D., Mieli-Vergani G., Alberti A., Neuberger J., Eddleston A. L., Davis M., Williams R. Antibodies to the surface of halothane-altered rabbit hepatocytes in patients with severe halothane-associated hepatitis. N Engl J Med. 1980 Jul 10;303(2):66–71. doi: 10.1056/NEJM198007103030202. [DOI] [PubMed] [Google Scholar]
  18. Volta U., Ballardini G., Cassani F., Zauli D., Bianchi F. B., Pisi E. Hépatite chronique active avec anticorps antimicrosome de foie et de rein. Med Chir Dig. 1980;9(6):465–469. [PubMed] [Google Scholar]
  19. Walton B., Simpson B. R., Strunin L., Doniach D., Perrin J., Appleyard A. J. Unexplained hepatitis following halothane. Br Med J. 1976 May 15;1(6019):1171–1176. doi: 10.1136/bmj.1.6019.1171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wälli F., Grob P. J., Müller-Schoop J. Pseudo-(Venocuran-)Lupus--eine kleine Episode Medizingeschichte. Schweiz Med Wochenschr. 1981 Sep 19;111(38):1398–1405. [PubMed] [Google Scholar]

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