Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1984 Apr;56(1):129–134.

Asialoagalactothyroglobulin binds to the surface of human thyroid cells at a site distinct from the 'microsomal' autoantigen.

I M Roitt, R Pujol-Borrell, T Hanafusa, P J Delves, G F Bottazzo, L D Kohn
PMCID: PMC1535972  PMID: 6201308

Abstract

Human thyroglobulin has been shown for the first time to bind to the surface of cultured human thyroid follicular cells. Binding was only observed with partially glycosylated asialoagalactothyroglobulin, not with the fully glycosylated iodoprotein. The binding site for asialoagalactothyroglobulin in the cell membrane is distinct from membrane associated microsomal/microvilli antigen. Since asialoagalactothyroglobulin bears the autoantigenic determinants of the parent molecule, its ability to bind to the thyroid cell surface suggests a possible role for this protein in the pathogenesis of human thyroiditis.

Full text

PDF
131

Images in this article

132Fig. 1
on p.132

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELYAVIN G., TROTTER W. R. Investigations of thyroid antigens reacting with Hashimoto sera; evidence for an antigen other than thyroglobulin. Lancet. 1959 Mar 28;1(7074):648–652. doi: 10.1016/s0140-6736(59)92401-8. [DOI] [PubMed] [Google Scholar]
  2. Bottazzo G. F., Pujol-Borrell R., Hanafusa T., Feldmann M. Role of aberrant HLA-DR expression and antigen presentation in induction of endocrine autoimmunity. Lancet. 1983 Nov 12;2(8359):1115–1119. doi: 10.1016/s0140-6736(83)90629-3. [DOI] [PubMed] [Google Scholar]
  3. Calder E. A., Penhale W. J., McLeman D., Barnes E. W., Irvine W. J. Lymphocyte-dependent antibody-mediated cytotoxicity in Hashimoto thyroiditis. Clin Exp Immunol. 1973 Jun;14(2):153–158. [PMC free article] [PubMed] [Google Scholar]
  4. Consiglio E., Shifrin S., Yavin Z., Ambesi-Impiombato F. S., Rall J. E., Salvatore G., Kohn L. D. Thyroglobulin interactions with thyroid membranes. Relationship between receptor recognition of N-acetylglucosamine residues and the iodine content of thyroglobulin preparations. J Biol Chem. 1981 Oct 25;256(20):10592–10599. [PubMed] [Google Scholar]
  5. Fenzi G. F., Bartalena L., Chiovato L., Marcocci C., Rotella C. M., Zonefrati R., Toccafondi R., Pinchera A. Studies on thyroid cell surface antigens using cultured human thyroid cells. Clin Exp Immunol. 1982 Feb;47(2):336–344. [PMC free article] [PubMed] [Google Scholar]
  6. Khoury E. L., Hammond L., Bottazzo G. F., Doniach D. Presence of the organ-specific 'microsomal' autoantigen on the surface of human thyroid cells in culture: its involvement in complement-mediated cytotoxicity. Clin Exp Immunol. 1981 Aug;45(2):316–328. [PMC free article] [PubMed] [Google Scholar]
  7. McLachlan S. M., Dickinson A. M., Malcolm A., Farndon J. R., Young E., Proctor S. J., Smith B. R. Thyroid autoantibody synthesis by cultures of thyroid and peripheral blood lymphocytes. I. Lymphocyte markers and response to pokeweed mitogen. Clin Exp Immunol. 1983 Apr;52(1):45–53. [PMC free article] [PubMed] [Google Scholar]
  8. Podleski W. K. Cytotoxic lymphocytes in Hashimoto's thyroiditis. Clin Exp Immunol. 1972 Aug;11(4):543–548. [PMC free article] [PubMed] [Google Scholar]
  9. Pujol-Borrell R., Hanafusa T., Chiovato L., Bottazzo G. F. Lectin-induced expression of DR antigen on human cultured follicular thyroid cells. Nature. 1983 Jul 7;304(5921):71–73. doi: 10.1038/304071a0. [DOI] [PubMed] [Google Scholar]
  10. ROITT I. M., LING N. R., DONIACH D., COUCHMAN K. G. THE CYTOPLASMIC AUTO-ANTIGEN OF THE HUMAN THYROID. I. IMMUNOLOGICAL AND BIOCHEMICAL CHARACTERISTICS. Immunology. 1964 Jul;7:375–393. [PMC free article] [PubMed] [Google Scholar]
  11. Reinherz E. L., Morimoto C., Penta A. C., Schlossman S. F. Regulation of B cell immunoglobulin secretion by functional subsets of T lymphocytes in man. Eur J Immunol. 1980 Jul;10(7):570–572. doi: 10.1002/eji.1830100715. [DOI] [PubMed] [Google Scholar]
  12. Shifrin S., Consiglio E., Laccetti P., Salvatore G., Kohn L. D. Bovine thyroglobulin. 27 S iodoprotein interactions with thyroid membranes and formation of a 27 S iodoprotein in vitro. J Biol Chem. 1982 Aug 25;257(16):9539–9547. [PubMed] [Google Scholar]
  13. Wall J. R., Baur R., Schleusener H., Bandy-Dafoe P. Peripheral blood and intrathyroidal mononuclear cell populations in patients with autoimmune thyroid disorders enumerated using monoclonal antibodies. J Clin Endocrinol Metab. 1983 Jan;56(1):164–169. doi: 10.1210/jcem-56-1-164. [DOI] [PubMed] [Google Scholar]
  14. Weetman A. P., McGregor A. M., Lazarus J. H., Hall R. Thyroid antibodies are produced by thyroid-derived lymphocytes. Clin Exp Immunol. 1982 Apr;48(1):196–200. [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES