Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1984 May;56(2):263–271.

Measurement of antibody to poly (adenosine diphosphate-ribose): its diagnostic value in systemic lupus erythematosus.

A L Clayton, R M Bernstein, M Tavassoli, S Shall, C Bunn, G R Hughes, S M Chantler
PMCID: PMC1536213  PMID: 6610511

Abstract

Poly (ADP-ribose) and dsDNA binding activity have been measured in sera from 61 patients with systemic lupus erythematosus (SLE) and 188 control sera from 20 normal individuals, 144 patients with clinically similar diseases and 24 patients with drug-induced anti-nuclear antibodies (ANA). Elevated poly (ADP-ribose) binding was not observed with normal sera. Five of 144 samples from diseases entering the differential diagnosis of SLE gave raised poly (ADP-ribose) binding compared with 12 in the 125I-dsDNA binding. Only two of these false positive samples gave elevated binding in the 14C-dsDNA assay. The apparent high specificity of the poly(ADP-ribose) assay was not observed with samples containing drug-induced ANA where 62% had elevated binding values. The frequency with which the poly(ADP-ribose) assay was positive with SLE sera (sensitivity) was lower than either of the dsDNA assays. This low sensitivity and the high rate of false positives in patients with drug-induced ANA limit the value of the poly(ADP-ribose) assay as a diagnostic test for SLE. However the restriction of poly(ADP-ribose) antibody to SLE and patients with drug-induced ANA together with the known role of poly(ADP-ribose) in DNA excision repair suggest that the antibody may be of fundamental significance.

Full text

PDF
270

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Durkacz B. W., Omidiji O., Gray D. A., Shall S. (ADP-ribose)n participates in DNA excision repair. Nature. 1980 Feb 7;283(5747):593–596. doi: 10.1038/283593a0. [DOI] [PubMed] [Google Scholar]
  2. FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
  3. Hayaishi O., Ueda K. Poly(ADP-ribose) and ADP-ribosylation of proteins. Annu Rev Biochem. 1977;46:95–116. doi: 10.1146/annurev.bi.46.070177.000523. [DOI] [PubMed] [Google Scholar]
  4. Holian J., Griffiths I. D., Glass D. N., Maini R. N., Scott J. T. Human anti-DNA antibody: reference standards for diagnosis and management of systemic lupus erythematosus. Ann Rheum Dis. 1975 Oct;34(5):438–443. doi: 10.1136/ard.34.5.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hughes G. R. Frequency of anti-DNA antibodies in SLE. RA and other diseases. Experience wiht the ammonium sulphate precipitation technique. Scand J Rheumatol Suppl. 1975;11:42–51. doi: 10.3109/03009747509095628. [DOI] [PubMed] [Google Scholar]
  6. Kanai Y., Kawaminami Y., Miwa M., Matsushima T., Sugimura T. Naturally-occurring antibodies to poly(ADP-ribose) in patients with systemic lupus erythematosus. Nature. 1977 Jan 13;265(5590):175–177. doi: 10.1038/265175a0. [DOI] [PubMed] [Google Scholar]
  7. Lange A. Evaluation of the simultaneous estimation of anti-dsDNA and anti-ssDNA antibodies for clinical purposes. Clin Exp Immunol. 1978 Mar;31(3):472–481. [PMC free article] [PubMed] [Google Scholar]
  8. Mansilla-Tinoco R., Harland S. J., Ryan P. J., Bernstein R. M., Dollery C. T., Hughes G. R., Bulpitt C. J., Morgan A., Jones J. M. Hydralazine, antinuclear antibodies, and the lupus syndrome. Br Med J (Clin Res Ed) 1982 Mar 27;284(6320):936–939. doi: 10.1136/bmj.284.6320.936. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morrow W. J., Isenberg D. A., Parry H. F., Shen L., Okolie E. E., Farzaneh F., Shall S., Snaith M. L. Studies on autoantibodies to poly (adenosine diphosphate-ribose) in SLE and other autoimmune diseases. Ann Rheum Dis. 1982 Aug;41(4):396–402. doi: 10.1136/ard.41.4.396. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Okolie E. E., Shall S. The significance of antibodies to poly(adenosine diphosphate-ribose) in systemic lupus erythematosus. Clin Exp Immunol. 1979 Apr;36(1):151–164. [PMC free article] [PubMed] [Google Scholar]
  11. Pincus T., Schur P. H., Rose J. A., Decker J. L., Talal N. Measurement of serum DNA-binding activity in systemic lupus erythematosus. N Engl J Med. 1969 Sep 25;281(13):701–705. doi: 10.1056/NEJM196909252811304. [DOI] [PubMed] [Google Scholar]
  12. Purnell M. R., Stone P. R., Whish W. J. ADP-ribosylation of nuclear proteins. Biochem Soc Trans. 1980 Apr;8(2):215–227. doi: 10.1042/bst0080215. [DOI] [PubMed] [Google Scholar]
  13. Schwartz R. S. Recent trends in systemic lupus erythematosus. Pharmacol Rev. 1982 Mar;34(1):105–108. [PubMed] [Google Scholar]
  14. Swaak A. J., Groenwold J., Aarden L. A., Statius van Eps L. W., Feltkamp E. W. Prognostic value of anti-dsDNA in SLE. Ann Rheum Dis. 1982 Aug;41(4):388–395. doi: 10.1136/ard.41.4.388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tan E. M. Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv Immunol. 1982;33:167–240. doi: 10.1016/s0065-2776(08)60836-6. [DOI] [PubMed] [Google Scholar]
  16. Tan E. M., Cohen A. S., Fries J. F., Masi A. T., McShane D. J., Rothfield N. F., Schaller J. G., Talal N., Winchester R. J. The 1982 revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1982 Nov;25(11):1271–1277. doi: 10.1002/art.1780251101. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES