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. 1981 Oct;46(1):20–26.

Nephrotoxic nephritis in nude mice.

Y Kusuyama, T Nishihara, K Saito
PMCID: PMC1536332  PMID: 6978213

Abstract

We investigated the role of T cells in the pathogenesis of murine nephrotoxic nephritis (NTN). The disease was produced by injecting congenitally athymic nude (nu/nu) mice and their normal heterozygous (nu/+) littermates with rabbit anti-rat glomerular basement membrane (GBM) antiserum. Within 2-4 weeks we noted marked thrombotic lesions and depositions of mouse IgG, IgM, C3 and rabbit IgG along the GBM in both groups of mice. There was no significant difference in the extent of glomerular involvement between the two groups of mice. We conclude that T cell immunodeficiency plays no role in the development of severe glomerular lesions in murine NTN.

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22FIG. 1
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23Fig. 2
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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhan A. K., Schneeberger E. E., Collins A. B., McCluskey R. T. Evidence for a pathogenic role of a cell-mediated immune mechanism in experimental glomerulonephritis. J Exp Med. 1978 Jul 1;148(1):246–260. doi: 10.1084/jem.148.1.246. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Danno K., Fukuyama K., Epstein W. L. Renal deposition of antinuclear antibody in athymic mice infected with Schistosoma mansoni. Lab Invest. 1979 Mar;40(3):358–363. [PubMed] [Google Scholar]
  3. Hong R., Schulte-Wissermann H., Jarrett-Toth E., Horowitz S. D., Manning D. D. Transplantation of cultured thymic fragments. II. Results in nude mice. J Exp Med. 1979 Feb 1;149(2):398–415. doi: 10.1084/jem.149.2.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ishikawa H., Saito K. Congenitally athymic nude (nu/nu) mice have Thy-1-bearing immunocompetent helper T cells in their peritoneal cavity. J Exp Med. 1980 Apr 1;151(4):965–968. doi: 10.1084/jem.151.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KRAKOWER C. A., GREENSPON S. A. Localization of the nephrotoxic antigen within the isolated renal glomerulus. AMA Arch Pathol. 1951 Jun;51(6):629–639. [PubMed] [Google Scholar]
  6. Kondo Y., Shigematsu H. Cellular aspects of rabbit Masugi nephritis. I. Cell kinetics in recoverable glomerulonephritis. Virchows Arch B Cell Pathol. 1972;10(1):40–50. doi: 10.1007/BF02899714. [DOI] [PubMed] [Google Scholar]
  7. Naish P. F., Thomson N. M., Simpson I. J., Peters D. K. The role of polymorphonuclear leucocytes in the autologous phase of nephrotoxic nephritis. Clin Exp Immunol. 1975 Oct;22(1):102–111. [PMC free article] [PubMed] [Google Scholar]
  8. Nishihara T., Kusuyama Y., Gen E., Tamaki N., Saito K. Masugi nephritis produced by the antiserum to heterologous glomerular basement membrane. I. Results in mice. Acta Pathol Jpn. 1981 Jan;31(1):85–92. doi: 10.1111/j.1440-1827.1981.tb00986.x. [DOI] [PubMed] [Google Scholar]
  9. Pantelouris E. M. Absence of thymus in a mouse mutant. Nature. 1968 Jan 27;217(5126):370–371. doi: 10.1038/217370a0. [DOI] [PubMed] [Google Scholar]
  10. Pelletier M., Hinglais N., Bach J. F. Characteristic immunohistochemical and ultrastructural glomerular lesions in Nude mice. Lab Invest. 1975 Mar;32(3):388–396. [PubMed] [Google Scholar]
  11. Sato V. L., Waksal S. D., Herzenberg L. A. Identification and separation of pre T-cells from nu/nu mice: differentiation by preculture with thymic reticuloepithelial cells. Cell Immunol. 1976 Jun 1;24(1):173–185. doi: 10.1016/0008-8749(76)90142-8. [DOI] [PubMed] [Google Scholar]
  12. Schreiner G. F., Cotran R. S., Pardo V., Unanue E. R. A mononuclear cell component in experimental immunological glomerulonephritis. J Exp Med. 1978 Feb 1;147(2):369–384. doi: 10.1084/jem.147.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shigematsu H., Kobayashi Y. The development and fate of the immune deposits in the glomerulus during the secondary phase of rat Masugi nephritis. Virchows Arch B Cell Pathol. 1971;8(2):83–95. doi: 10.1007/BF02893517. [DOI] [PubMed] [Google Scholar]
  14. Shimizu F. Persistent Masugi nephritis induced in rats in spite of the suppression of host antibody responses against the heterologous nephrotoxic antibodies. Jpn J Exp Med. 1974 Oct;44(5):443–449. [PubMed] [Google Scholar]
  15. Sindrey M., Marshall T. L., Naish P. Quantitative assessment of the effects of platelet depletion in the autologous phase of nephrotoxic serum nephritis. Clin Exp Immunol. 1979 Apr;36(1):90–96. [PMC free article] [PubMed] [Google Scholar]
  16. Unanue E. R., Mardiney M. R., Jr, Dixon F. J. Nephrotoxic serum nephritis in complement intact and deficient mice. J Immunol. 1967 Mar;98(3):609–617. [PubMed] [Google Scholar]
  17. Wilson C. B., Dixon F. J., Fortner J. G., Cerilli G. J. Glomerular basement membrane--reactive antibody in anti-lymphocyte globulin. J Clin Invest. 1971 Jul;50(7):1525–1535. doi: 10.1172/JCI106638. [DOI] [PMC free article] [PubMed] [Google Scholar]

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