Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 May;48(2):321–328.

Detection of virus-specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection by enzymeimmuno and radioimmunoassay techniques.

I Sarov, E Levy, M Aymard, Y Chardonnet, S Bosshard, J P Revillard, M Friedman, E Nord, M Greiff, H Haikin
PMCID: PMC1536472  PMID: 6286188

Abstract

The feasibility of using human cytomegalovirus (CMV)-specific IgA antibody determinations as a signal for early detection of recurrent CMV infections in eight renal transplant recipients was analyzed. Solid phase radioimmunoassay (RIA), enzyme-liked immunosorbent assay (ELISA) and immunoperoxidase assay (IPA) techniques were used for IgA antibody determinations. In parallel, IgG antibodies to CMV were studied by immunoperoxidase assay. A significant rise of CMV-specific IgG antibody titre was observed in all of these patients between 5 and 53 weeks post-transplantation. CMV-specific IgA antibody production was detected close to the time a rise in CMV IgG antibody was observed in seven out of eight patients studied by RIA and ELISA, and in six out of eight patients studied by IPA. In two patients specific CMV IgA antibodies were detected by all three methods before a significant rise of CMV IgG antibody titre was demonstrated. In these patients CMV IgA was detected by RIA earlier than by ELISA and IPA. The potential application of CMV-specific IgA antibody determination for early detection of recurrent CMV infection in renal transplant patients is discussed.

Full text

PDF
328

Images in this article

325Fig. 1
on p.325

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen P., Andersen H. K. Immunoglobulin levels and specific viral antibodies in relation to smooth-muscle antibodies in cytomegalovirus infection. J Clin Lab Immunol. 1978 Nov;1(3):233–236. [PubMed] [Google Scholar]
  2. Betts R. F., Freeman R. B., Douglas R. G., Jr, Talley T. E. Clinical manifestations of renal allograft derived primary cytomegalovirus infection. Am J Dis Child. 1977 Jul;131(7):759–763. doi: 10.1001/archpedi.1977.02120200041010. [DOI] [PubMed] [Google Scholar]
  3. Betts R. F., Freeman R. B., Douglas R. G., Jr, Talley T. E., Rundell B. Transmission of cytomegalovirus infection with renal allograft. Kidney Int. 1975 Dec;8(6):385–392. doi: 10.1038/ki.1975.131. [DOI] [PubMed] [Google Scholar]
  4. Brunell P. A., Gershon A. A., Uduman S. A., Steinberg S. Varicella-Zoster Immunoglobulins during Varicella, Latency, and Zoster. J Infect Dis. 1975 Jul;132(1):49–54. doi: 10.1093/infdis/132.1.49. [DOI] [PubMed] [Google Scholar]
  5. Dowling P., Menonna J., Cook S. Cytomegalovirus complement fixation antibody in Guillain-Barré syndrome. Neurology. 1977 Dec;27(12):1153–1156. doi: 10.1212/wnl.27.12.1153. [DOI] [PubMed] [Google Scholar]
  6. Friedman M. G., Leventon-Kriss S., Sarov I. Sensitive solid-phase radioimmunoassay for detection of human immunoglobulin G antibodies to varicella-zoster virus. J Clin Microbiol. 1979 Jan;9(1):1–10. doi: 10.1128/jcm.9.1.1-10.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gerna G., Chambers R. W. Rapid detection of human cytomegalovirus and herpesvirus hominis IgM antibody by the immunoperoxidase technique. Intervirology. 1977;8(5):257–271. doi: 10.1159/000148901. [DOI] [PubMed] [Google Scholar]
  8. Giraldo G., Beth E., Huang E. S. Kaposi's sarcoma and its relationship to cytomegalovirus (CMNV). III. CMV DNA and CMV early antigens in Kaposi's sarcoma. Int J Cancer. 1980 Jul 15;26(1):23–29. doi: 10.1002/ijc.2910260105. [DOI] [PubMed] [Google Scholar]
  9. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  10. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  11. Haikin H., Leventon-Kriss S., Sarov I. Antibody to varicella-zoster virus-induced membrane antigen: immunoperoxidase assay with air-dried target cells. J Infect Dis. 1979 Oct;140(4):601–604. doi: 10.1093/infdis/140.4.601. [DOI] [PubMed] [Google Scholar]
  12. Haikin H., Sarov I. Immunoperoxidase antibody to human cytomegalovirus-induced membrane antigen assay in the absence of interfering immunoglobulin G receptors. Intervirology. 1980;14(3-4):155–159. doi: 10.1159/000149177. [DOI] [PubMed] [Google Scholar]
  13. Hamilton J. R., Overall J. C., Glasgow L. A. Synergistic effect on mortality in mice with murine cytomegalovirus and Pseudomonas aeruginosa, Staphylococcus aureus, or Candida albicans infections. Infect Immun. 1976 Oct;14(4):982–989. doi: 10.1128/iai.14.4.982-989.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hekker A. C., Brand-Saathof B., Vis J., Meijers R. C. Indirect immunofluorescence test for detection of IgM antibodies to cytomegalovirus. J Infect Dis. 1979 Oct;140(4):596–600. doi: 10.1093/infdis/140.4.596. [DOI] [PubMed] [Google Scholar]
  15. Ho M., Suwansirikul S., Dowling J. N., Youngblood L. A., Armstrong J. A. The transplanted kidney as a source of cytomegalovirus infection. N Engl J Med. 1975 Nov 27;293(22):1109–1112. doi: 10.1056/NEJM197511272932201. [DOI] [PubMed] [Google Scholar]
  16. Ho M. Virus infections after transplantation in man. Brief review. Arch Virol. 1977;55(1-2):1–24. doi: 10.1007/BF01314475. [DOI] [PubMed] [Google Scholar]
  17. Kahane S., Dvilansky A., Estok L., Nathan I., Zolotov Z., Sarov I. Detection of anti-platelet antibodies in patients with idiopathic thrombocytopenic purpura (ITP) and in patients with rubella and herpes group viral infections. Clin Exp Immunol. 1981 Apr;44(1):49–56. [PMC free article] [PubMed] [Google Scholar]
  18. Kangro H. O. Evaluation of a radioimmunoassay for IgM-class antibodies against cytomegalovirus. Br J Exp Pathol. 1980 Oct;61(5):512–520. [PMC free article] [PubMed] [Google Scholar]
  19. Kimmel N., Friedman M. G., Sarov I. Detection of human cytomegalovirus-specific IgG antibodies by a sensitive solid-phase radioimmunoassay and by a rapid-screening test. J Med Virol. 1980;5(3):195–203. doi: 10.1002/jmv.1890050304. [DOI] [PubMed] [Google Scholar]
  20. Knez V., Stewart J. A., Ziegler D. W. Cytomegalovirus specific IgM and IgG response in humans studied by radioimmunoassay. J Immunol. 1976 Nov;117(5 PT2):2006–2013. [PubMed] [Google Scholar]
  21. Krishna R. V., Meurman O. H., Ziegler T., Krech U. H. Solid-phase enzyme immunoassay for determination of antibodies to cytomegalovirus. J Clin Microbiol. 1980 Jul;12(1):46–51. doi: 10.1128/jcm.12.1.46-51.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  23. Levy E., Sarov I. Detection of specific IgA antibodies in serum of patients with varicella and zoster infections. Intervirology. 1981;15(2):103–110. doi: 10.1159/000149220. [DOI] [PubMed] [Google Scholar]
  24. Levy E., Sarov I. Determination of IgA antibodies to human cytomegalovirus by enzyme-linked immunosorbent assay (ELISA). J Med Virol. 1980;6(3):249–257. doi: 10.1002/jmv.1890060308. [DOI] [PubMed] [Google Scholar]
  25. Link H., Wahren B., Norrby E. Pleocytosis and immunoglobulin changes in cerebrospinal fluid and herpesvirus serology in patients with Guillain-Barré syndrome. J Clin Microbiol. 1979 Mar;9(3):305–316. doi: 10.1128/jcm.9.3.305-316.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Marker S. C., Howard R. J., Groth K. E., Mastri A. R., Simmons R. L., Balfour H. H., Jr A trial of vidarabine for cytomegalovirus infection in renal transplant patients. Arch Intern Med. 1980 Nov;140(11):1441–1444. [PubMed] [Google Scholar]
  27. Pass R. F., Whitley R. J., Diethelm A. G., Whelchel J. D., Reynolds D. W., Alford C. A., Jr Outcome of renal transplantation in patients with primary cytomegalovirus infection. Transplant Proc. 1979 Jun;11(2):1288–1290. [PubMed] [Google Scholar]
  28. Sarov I., Andersen P., Andersen H. K. Enzyme linked immunosorbent assay (ELISA) for determination of IgG antibodies to human cytomegalovirus. Acta Pathol Microbiol Scand B. 1980 Feb;88(1):1–9. doi: 10.1111/j.1699-0463.1980.tb02597.x. [DOI] [PubMed] [Google Scholar]
  29. Sarov I., Siqueira-Linhares M., Chardonnet Y., Levy E., Aymard M., Bosshard S., Nord E., Revillard J. P. Detection of specific IgA antibodies in serum of kidney transplant patients with recurrent cytomegalovirus infection. Intervirology. 1981;15(4):228–234. doi: 10.1159/000149236. [DOI] [PubMed] [Google Scholar]
  30. Schmitz H., Doerr H. W., Kampa D., Vogt A. Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus. J Clin Microbiol. 1977 Jun;5(6):629–634. doi: 10.1128/jcm.5.6.629-634.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schmitz H., Enders G. Cytomegalovirus as a frequent cause of Guillain-Barré syndrome. J Med Virol. 1977;1(1):21–27. doi: 10.1002/jmv.1890010105. [DOI] [PubMed] [Google Scholar]
  32. Schmitz H., Haas R. Determination of different cytomegalovirus immunoglobulins (IgG, IgA, IgM) by immunofluorescence. Arch Gesamte Virusforsch. 1972;37(1):131–140. doi: 10.1007/BF01241158. [DOI] [PubMed] [Google Scholar]
  33. The T. H., Andersen H. K., Spencer E. S., Klein G. Antibodies against cytomegalovirus-induced early antigens (CMV-EA) in immunosuppressed renal-allograft recipients. Clin Exp Immunol. 1977 Jun;28(3):502–505. [PMC free article] [PubMed] [Google Scholar]
  34. Torfason E. G., Källander C., Halonen P. Solid-phase radioimmunoassay of serum IgG, IgM, and IgA antibodies to cytomegalovirus. J Med Virol. 1981;7(2):85–96. doi: 10.1002/jmv.1890070202. [DOI] [PubMed] [Google Scholar]
  35. Whelchel J. D., Pass R. F., Diethelm A. G., Whitley R. J., Alford C. A., Jr Effect of primary and recurrent cytomegalovirus infections upon graft and patient survival after renal transplantation. Transplantation. 1979 Dec;28(6):443–446. [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES