Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 Aug;49(2):419–425.

Detection of polymeric IgA in glomeruli from patients with IgA nephropathy.

Y Tomino, H Sakai, M Miura, M Endoh, Y Nomoto
PMCID: PMC1536483  PMID: 6813005

Abstract

A study on the detection of polymeric IgA in glomeruli from renal biopsy specimens in patients with IgA nephropathy is described. Renal biopsy specimens were obtained from patients with IgA nephropathy. These specimens were stained with FITC-labelled anti-human J chain antisera and then examined with a fluorescent microscope. The J chain was observed in the glomerular mesangium by immunofluorescent staining. In parallel studies, renal biopsy specimens were treated with citrate buffer (pH 3.2) and the 'eluate' was neutralized by sodium hydroxide. The eluate was labelled with iodine-125, and the radiolabelled 'eluate' was fractionated by sucrose density-gradient ultracentrifugation. Polymerized IgA in the 'eluate' obtained from patients with IgA nephropathy was found to sediment predominantly as 9S to 11S using a sucrose density gradient analysis. Polymeric IgA in the fractions of the density gradient analysis was determined by anti-human IgA and anti-human J chain antisera. It was demonstrated that IgA and J chain were eluted from the glomeruli in some patients with IgA nephropathy. It is concluded that IgA deposited in the glomeruli is composed of dimers and/or larger polymers of circulating IgA in some patients with IgA nephropathy.

Full text

PDF
423

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berger J. IgA glomerular deposits in renal disease. Transplant Proc. 1969 Dec;1(4):939–944. [PubMed] [Google Scholar]
  2. Berger J., Yaneva H., Nabarra B., Barbanel C. Recurrence of mesangial deposition of IgA after renal transplantation. Kidney Int. 1975 Apr;7(4):232–241. doi: 10.1038/ki.1975.35. [DOI] [PubMed] [Google Scholar]
  3. Brandtzaeg P. Studies on J chain and binding site for secretory component in circulating human B cells. II. The cytoplasm. Clin Exp Immunol. 1976 Jul;25(1):59–66. [PMC free article] [PubMed] [Google Scholar]
  4. Clarkson A. R., Seymour A. E., Woodroffe A. J., McKenzie P. E., Chan Y. L., Wootton A. M. Controlled trial of phenytoin therapy in IgA nephropathy. Clin Nephrol. 1980 May;13(5):215–218. [PubMed] [Google Scholar]
  5. Conley M. E., Cooper M. D., Michael A. F. Selective deposition of immunoglobulin A1 in immunoglobulin A nephropathy, anaphylactoid purpura nephritis, and systemic lupus erythematosus. J Clin Invest. 1980 Dec;66(6):1432–1436. doi: 10.1172/JCI109998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kobayashi K., Vaerman J. P., Bazin H., LeBacq-Verheyden A. M., Heremans J. F. Identification of J-chain in polymeric immunoglobulins from a variety of species by cross-reaction with rabbit antisera to human J-chain. J Immunol. 1973 Nov;111(5):1590–1594. [PubMed] [Google Scholar]
  7. Korsrud F. R., Brandtzaeg P. Immunohistochemical evaluation of J-chain expression by intra- and extra-follicular immunoglobulin-producing human tonsillar cells. Scand J Immunol. 1981;13(3):271–280. doi: 10.1111/j.1365-3083.1981.tb00135.x. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Laurent G., Delsol G., Reyes F., Abbal M., Mihaesco E. Detection of J chain in lymphomas and related disorders. Clin Exp Immunol. 1981 Jun;44(3):620–628. [PMC free article] [PubMed] [Google Scholar]
  10. Lowance D. C., Mullins J. D., McPhaul J. J., Jr IgA-Associated glomerulonepbritis. Int Rev Exp Pathol. 1977;17:143–172. [PubMed] [Google Scholar]
  11. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  12. McCoy R. C., Abramowsky C. R., Tisher C. C. IgA nephropathy. Am J Pathol. 1974 Jul;76(1):123–144. [PMC free article] [PubMed] [Google Scholar]
  13. Nagura H., Brandtzaeg P., Nakane P. K., Brown W. R. Ultrastructural localization of J chain in human intestinal mucosa. J Immunol. 1979 Sep;123(3):1044–1050. [PubMed] [Google Scholar]
  14. Shirai T., Tomino Y., Sato M., Yoshiki T., Itoh T. IgA nephropathy: clinicopathology and immunopathology. Contrib Nephrol. 1978;9:88–100. doi: 10.1159/000401436. [DOI] [PubMed] [Google Scholar]
  15. Tomino Y., Endoh M., Nomoto Y., Sakai H. Immunoglobulin A1 and IgA nephropathy. N Engl J Med. 1981 Nov 5;305(19):1159–1160. doi: 10.1056/NEJM198111053051922. [DOI] [PubMed] [Google Scholar]
  16. Tomino Y., Endoh M., Nomoto Y., Sakai H. Specificity of eluted antibody from renal tissues of patients with IgA nephropathy. Am J Kidney Dis. 1982 Mar;1(5):276–280. doi: 10.1016/s0272-6386(82)80025-5. [DOI] [PubMed] [Google Scholar]
  17. Tomino Y., Nomoto Y., Endoh M., Sakai H. Evaluation of the staining conditions of immunofluorescence in primary glomerulonephritis. Acta Pathol Jpn. 1981 Jan;31(1):99–104. doi: 10.1111/j.1440-1827.1981.tb00988.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES