Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1982 Feb;47(2):396–404.

Two maturation-associated mouse erythrocyte receptors of human B cells. I. Identification of four human B-cell subsets.

I J Forbes, P D Zalewski, L Valente, D Gee
PMCID: PMC1536537  PMID: 6978783

Abstract

Using rosetting tests with untreated mouse erythrocytes (M) and pronase-treated M (pro M), four human B cell subsets can be identified. Three of these, possessing the phenotypes BM+ pro M+, BM- pro M+ or BM- pro M-, constitute 17%, 61% and 22% of normal blood B cells respectively. The fourth subset, BM+ pro M-, does not occur in normal tissues but was found in the pre-B-cell line of Raji cells, indicating that this phenotype may be a marker for early B cells. Some differences in the proportion of each subset were found in cord blood, lymph nodes and tonsils. Surface-immunoglobulin-positive (SIg+) and -negative (SIg-) non-T cells were present in each subset. M and pro-M rosetting tests were applied to cells from blood of 27 cases of chronic lymphocytic leukaemia (CLL) and to cells from involved nodes, spleen or marrow in five cases of non-Hodgkin's lymphoma (NHL). In 15 cases of CLL, there was considerable increase in the BM+ pro M+ subset (BM+ pro M+ type CLL); in seven cases, there was a predominance of BM- pro M+ cells and in another four cases, BM- pro M- cells predominated. All five cases of NHL were greatly enriched in BM- pro M- cells. There was no obvious correlation between rosetting and other surface markers but BM- pro M- clones in CLL or NHL always stained brightly with FITC-anti-Ig. This was not found in BM+ pro M+ or BM- pro M+ clones. Rosette formation of neuraminidase-treated B cells with M identifies the same subset as B-pro-M rosetting in normals and CLL. Evidence is presented that two types of receptors are involved in M and pro-M rosetting, designated R1 and R2, binding to corresponding M ligands L1 and L2. M rosetting is due to R1-L1 binding while R2-L2 binding mediates B-pro-M rosetting. Shifts between subsets within the same clone in some cases of CLL suggest that the subsets are distinct maturational stage of B-cell development rather than families of B cells of different lineage. The following B-cell maturation sequence is proposed: R1+ R2- lead to R1+ R2+ leads to R1- R2+ leads to R1- R2-.

Full text

PDF
404

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bertoglio J., Laldjim B., Doré J. F. Mouse erythrocyte rosette formation by human lymphoid cell lines. Scand J Immunol. 1979;10(2):153–159. doi: 10.1111/j.1365-3083.1979.tb03270.x. [DOI] [PubMed] [Google Scholar]
  2. Black R. B., Leong A. S., Cowled P. A., Forbes I. J. Lymphocyte subpopulations in human lymph nodes: a normal range. Lymphology. 1980 Jun;13(2):86–90. [PubMed] [Google Scholar]
  3. Catovsky D., Cherchi M., Okos A., Hegde U., Galton D. A. Mouse red-cell rosettes in B-lymphoproliferative disorders. Br J Haematol. 1976 Jun;33(2):173–177. doi: 10.1111/j.1365-2141.1976.tb03528.x. [DOI] [PubMed] [Google Scholar]
  4. Cordier G., Samarut C., Revillard J. P. Changes of Fcgamma receptor-related properties induced by interaction of human lymphocytes with insoluble immune complexes. J Immunol. 1977 Dec;119(6):1943–1948. [PubMed] [Google Scholar]
  5. Forbes I. J., Zalewski P. D. A subpopulation of human B lymphocytes that rosette with mouse erythrocytes. Clin Exp Immunol. 1976 Oct;26(1):99–107. [PMC free article] [PubMed] [Google Scholar]
  6. Forbes I. J., Zalewski P. D., Cowled P. A., Sage R. E. Maturation in B lymphocytic leukaemia. J Clin Lab Immunol. 1979 Feb;1(4):329–331. [PubMed] [Google Scholar]
  7. Forbes I. J., Zalewski P. D., Leong A. S., Sage R. E., Dale B., Cowled P. A. B cell leukaemia distinguished from chronic lymphocytic leukaemia by surface markers. Aust N Z J Med. 1978 Oct;8(5):532–538. doi: 10.1111/j.1445-5994.1978.tb02595.x. [DOI] [PubMed] [Google Scholar]
  8. Gupta S., Good R. A., Siegal F. P. Rosette-formation with mouse erythrocytes. II. A marker for human B and non-T lymphocytes. Clin Exp Immunol. 1976 Aug;25(2):319–327. [PMC free article] [PubMed] [Google Scholar]
  9. Gupta S., Pahwa R., O'Reilly R., Good R. A., Siegal F. P. Ontogeny of lymphocyte subpopulations in human fetal liver. Proc Natl Acad Sci U S A. 1976 Mar;73(3):919–922. doi: 10.1073/pnas.73.3.919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leong A. S., Forbes I. J., Cowled P. A., Sage R. E., Black R. B., Dale B., Zalewski P. D. Surface marker studies in chronic lymphocytic leukaemia and non-Hodgkin's lymphoma. Pathology. 1979 Jul;11(3):461–471. doi: 10.3109/00313027909059023. [DOI] [PubMed] [Google Scholar]
  11. Salmon S. E., Seligmann M. B-cell neoplasia in man. Lancet. 1974 Nov 23;2(7891):1230–1233. doi: 10.1016/s0140-6736(74)90748-x. [DOI] [PubMed] [Google Scholar]
  12. Zalewski P. D., Forbes I. J. Studies of the human lymphocyte-mouse erythrocyte bond. Clin Exp Immunol. 1979 Jun;36(3):536–546. [PMC free article] [PubMed] [Google Scholar]
  13. Zola H. Fractionation of human lymphocytes using rosette formation with papain-treated mouse erythrocytes. J Immunol Methods. 1977;18(3-4):387–389. doi: 10.1016/0022-1759(77)90193-4. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES