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. 1982 Feb;47(2):474–480.

In vitro monocyte-lymphocyte interaction influenced by d-penicillamine.

E De Vries, C J Haasnoot, J P Van der Weij, A Cats
PMCID: PMC1536549  PMID: 7075029

Abstract

The in vitro effect of d-penicillamine (Pen) on human peripheral blood mononuclear cells (PBMC) was studied in relation to pokeweed mitogen (PWM) stimulation. Incubation of PBMC with Pen during or after PWM stimulation resulted in only a slight decrease in blast-cell formation. This effect was dependent on the concentration of Pen. Preincubation of PBMC with 100 micrograms Pen/ml medium for 10 days led to a toxic effect on lymphoid cells and selective survival of mononuclear phagocytes (MNP). Preincubation with 10 and 50 micrograms Pen/ml medium resulted in limited spontaneous blast-cell formation, which did not occur after preincubation with 100 micrograms Pen/ml and 5 micrograms Pen/ml. Despite the decrease in the total number of lymphocytes after 10 days of Pen preincubation, blast-cell formation on subsequent PWM stimulation in the absence of Pen was considerably enhanced compared to controls. The blast-cell formation was suppressed when PWM stimulation of Pen-preincubated PBMC was performed in the presence of Pen. This suppression of blast-cell formation under stimulation in the presence of Pen might explain the therapeutic effect of Pen.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binderup L., Bramm E., Arrigoni-Martelli E. D-penicillamine and macrophages: modulation of lymphocyte transformation by concanavalin A. Scand J Immunol. 1978 Apr;7(4):259–264. doi: 10.1111/j.1365-3083.1978.tb00453.x. [DOI] [PubMed] [Google Scholar]
  2. Binderup L., Bramm E., Arrigoni-Martelli E. D-penicillamine in vivo enhances lymphocyte DNA synthesis: role of macrophages. Scand J Immunol. 1980;11(1):23–28. doi: 10.1111/j.1365-3083.1980.tb00204.x. [DOI] [PubMed] [Google Scholar]
  3. Hunneyball I. M., Stewart G. A., Stanworth D. R. Effect of D(-)penicillamine on chronic experimental arthritis in rabbits. Ann Rheum Dis. 1977 Aug;36(4):378–380. doi: 10.1136/ard.36.4.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hunneyball I. M., Stewart G. A., Stanworth D. R. The effects of oral D-penicillamine treatment on experimental arthritis and the associated immune response in rabbits. II. The effects on cellular parameters. Immunology. 1978 Jul;35(1):159–166. [PMC free article] [PubMed] [Google Scholar]
  5. Lipsky P. E., Ziff M. Inhibition of human helper T cell function in vitro by D-penicillamine and CuSO4. J Clin Invest. 1980 May;65(5):1069–1076. doi: 10.1172/JCI109759. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Lipsky P. E., Ziff M. The effect of D-penicillamine on mitogen-induced human lymphocyte proliferation: synergistic inhibition by D-penicillamine and copper salts. J Immunol. 1978 Mar;120(3):1006–1013. [PubMed] [Google Scholar]
  7. Liyanage S. P., Currey H. L. Failure of oral D-penicillamine to modify adjuvant arthritis or immune response in the rat. Ann Rheum Dis. 1972 Nov;31(6):521–521. doi: 10.1136/ard.31.6.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. McClelland D. B., van Furth R. In vitro synthesis of lysozyme by human and mouse tissues and leucocytes. Immunology. 1975 Jun;28(6):1099–1114. [PMC free article] [PubMed] [Google Scholar]
  9. Püschel W., Rosenkranz M., Geiler G., Caffier B., Stiehl P., Richter V. Zur Wirkung von D-Penicillamin auf die experimentelle allergische Arthritis des Kaninchens. Z Rheumatol. 1976 May-Jun;35(5-6):201–209. [PubMed] [Google Scholar]
  10. Schumacher K., Maerker-Alzer G., Preuss R. Effect of D-penicillamine on lymphocyte function. Arzneimittelforschung. 1975 Apr;25(4):603–606. [PubMed] [Google Scholar]
  11. TOBIN M. S., ALTMAN K. ACCELERATED IMMUNE RESPONSE INDUCED BY D-L PENICILLAMINE. Proc Soc Exp Biol Med. 1964 Jan;115:225–228. doi: 10.3181/00379727-115-28876. [DOI] [PubMed] [Google Scholar]
  12. de Vries E., Lafeber G. J., van der Weij J. P., van Buijsen A. C., Leijh P. C., Cats A. Pokeweed-mitogen induced lymphocyte proliferation: the effect of stimulation on mononuclear phagocytic cells. Immunology. 1980 Jun;40(2):177–182. [PMC free article] [PubMed] [Google Scholar]
  13. de Vries E., van de Putte L. B., Lafeber G. J., van Buysen-Nutters A. C., de Vetten-van der Hulst H. L., Meyer C. J. Morphological differences in the interactions between human mononuclear cells and coated or uncoated sheep red blood cells: a freeze-etch study of different types of rosettes. Cell Tissue Res. 1976 Apr 28;168(1):79–88. doi: 10.1007/BF00219725. [DOI] [PubMed] [Google Scholar]

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