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. 1980 Jul;41(1):8–12.

The elution of IgG from subacute sclerosing panencephalitis and multiple sclerosis brains.

C J Sindic, C L Cambiaso, P L Masson, E C Laterre
PMCID: PMC1536930  PMID: 7438551

Abstract

IgG was extracted from sections of a multiple sclerosis brain by repeated washing with phosphate-buffered saline. After this extraction, no IgG was recovered by further washing at pH 2.2. This indicates that the IgG of the plaques has no strong affinity for insoluble constituents of the brain. Section of a subacute sclerosing panencephalitis brain were used as positive controls: after repeated extraction with phosphate-buffered saline, IgG with anti-measles antibody activity was eluted at acid pH.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cambiaso C. L., Leek A. E., De Steenwinkel F., Billen J., Masson P. L. Particle counting immunoassay (PACIA). I. A general method for the determination of antibodies, antigens, and haptens. J Immunol Methods. 1977;18(1-2):33–44. doi: 10.1016/0022-1759(77)90156-9. [DOI] [PubMed] [Google Scholar]
  2. Hay F. C., Nineham L. J., Torrigiani G., Roitt I. M. Hidden' IgG antiglobulins in normal human serum. Clin Exp Immunol. 1976 Aug;25(2):185–190. [PMC free article] [PubMed] [Google Scholar]
  3. Izui S., Eisenberg R. A., Dixon F. J. IgM rheumatoid factors in mice injected with bacterial lipopolysaccharides. J Immunol. 1979 May;122(5):2096–2102. [PubMed] [Google Scholar]
  4. Simpson J. F., Tourtellotte W. W., Kokmen E., Parker J. A., Itabashi H. H. Fluorescent protein tracing in multiple sclerosis brain tissue. Arch Neurol. 1969 Apr;20(4):373–377. doi: 10.1001/archneur.1969.00480100049007. [DOI] [PubMed] [Google Scholar]
  5. Sindic C. J., Cambiaso C. L., Masson P. L., Laterre E. C. The binding of myelin basic protein to the Fc region of aggregated IgG and to immune complexes. Clin Exp Immunol. 1980 Jul;41(1):1–7. [PMC free article] [PubMed] [Google Scholar]
  6. Tavolato B. F. Immunoglobulin G distribution in multiple sclerosis brain. An immunofluorescence study. J Neurol Sci. 1975 Jan;24(1):1–11. doi: 10.1016/0022-510x(75)90002-7. [DOI] [PubMed] [Google Scholar]
  7. Tourtellotte W. W., Parker J. A. Multiple sclerosis: brain immunoglobulin-G and albumin. Nature. 1967 May 13;214(5089):683–686. doi: 10.1038/214683a0. [DOI] [PubMed] [Google Scholar]
  8. Weil M. L., Leiva W. A., Heiner D. C., Tourtellotte W. W. Release of bound immunoglobulin from SSPE brain by acid elution. J Immunol. 1975 Dec;115(6):1603–1606. [PubMed] [Google Scholar]
  9. Woyciechowska J. L., Brzosko W. J. Immunofluorescence study of brain plaques from two patients with multiple sclerosis. Neurology. 1977 Jul;27(7):620–622. doi: 10.1212/wnl.27.7.620. [DOI] [PubMed] [Google Scholar]

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