Skip to main content
NCBI home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1981 Mar;43(3):435–442.

A method for following human lymphocyte traffic using indium-111 oxine labelling.

J Wagstaff, C Gibson, N Thatcher, W L Ford, H Sharma, W Benson, D Crowther
PMCID: PMC1537198  PMID: 7285387

Abstract

A method is described whereby large numbers of human lymphocytes are separated from peripheral blood and labelled in vitro with indium-111 oxine. Following autologous reinjection, the distribution within the body is followed by means of serial blood samples, surface-probe counting and gamma camera imaging. The distribution of radioactivity following reinjection of heat-damaged labelled lymphocytes and free indium-111 oxine is different from that of 'normal' lymphocytes. The results suggest that the separation and labelling procedure does not cause significant physical damage to the lymphocytes The importance of restricting the specific lymphocyte activity to 20-40 microCi per 10(8) cells in order to minimize radiation damage to the lymphocytes is emphasized. Good resolution of lymphoid structures is obtained using gamma camera imaging and the changes recorded in organ distribution correlate well with data from animal models of lymphocyte migration. Thus, indium-111 oxine labelling of human lymphocytes provides a non-invasive method whereby the migratory properties of human lymphocytes can be followed.

Full text

PDF
435

Images in this article

439Fig. 2
on p.439
439Fig. 3
on p.439
439Fig. 4
on p.439

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. E., Warner N. L. Ionizing radiation and the immune response. Adv Immunol. 1976;24:215–335. doi: 10.1016/s0065-2776(08)60331-4. [DOI] [PubMed] [Google Scholar]
  2. Bradfield J. W., Born G. V. The migration of rat thoracic duct lymphocytes through spleen in vivo. Br J Exp Pathol. 1973 Oct;54(5):509–517. [PMC free article] [PubMed] [Google Scholar]
  3. Chisholm P. M., Danpure H. J., Healey G., Osman S. Cell damage resulting from the labeling of rat lymphocytes and HeLa S3 cells with In-111 oxine. J Nucl Med. 1979 Dec;20(12):1308–1311. [PubMed] [Google Scholar]
  4. Ford W. L. Lymphocyte migration and immune responses. Prog Allergy. 1975;19:1–59. doi: 10.1159/000313381. [DOI] [PubMed] [Google Scholar]
  5. Ford W. L. The immunological and migratory properties of the lymphocytes recirculating through the rat spleen. Br J Exp Pathol. 1969 Jun;50(3):257–269. [PMC free article] [PubMed] [Google Scholar]
  6. Frost H., Frost P., Wilcox C., Thrall J. Lymph node scanning in sheep with indium-111-labeled lymphocytes. Int J Nucl Med Biol. 1979;6(1):60–67. doi: 10.1016/0047-0740(79)90070-6. [DOI] [PubMed] [Google Scholar]
  7. Goodwin D. A. Cell labeling with oxine chelates of radioactive metal ions: techniques and clinical implications. J Nucl Med. 1978 May;19(5):557–559. [PubMed] [Google Scholar]
  8. Hersey P. The separation and 51 chromium labeling of human lymphocytes with in vivo studies of survival and migration. Blood. 1971 Sep;38(3):360–371. [PubMed] [Google Scholar]
  9. Issekutz T., Chin W., Hay J. B. Measurement of lymphocyte traffic with indium-111. Clin Exp Immunol. 1980 Jan;39(1):215–221. [PMC free article] [PubMed] [Google Scholar]
  10. Jønsson V., Christensen B. E. 51Cr labelling of normal human T and B lymphocytes for kinetic studies in vivo. Fundamental lymphocyte kinetic considerations. Scand J Haematol. 1978 Apr;20(4):319–329. doi: 10.1111/j.1600-0609.1978.tb02463.x. [DOI] [PubMed] [Google Scholar]
  11. Lavender J. P., Goldman J. M., Arnot R. N., Thakur M. L. Kinetics of indium-III labelled lymphocytes in normal subjects and patients with Hodgkin's disease. Br Med J. 1977 Sep 24;2(6090):797–799. doi: 10.1136/bmj.2.6090.797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McAfee J. G., Thakur M. L. Survey of radioactive agents for in vitro labeling of phagocytic leukocytes. I. Soluble agents. J Nucl Med. 1976 Jun;17(6):480–487. [PubMed] [Google Scholar]
  13. Rannie G. H., Thakur M. L., Ford W. L. An experimental comparison of radioactive labels with potential application to lymphocyte migration studies in patients. Clin Exp Immunol. 1977 Sep;29(3):509–514. [PMC free article] [PubMed] [Google Scholar]
  14. Scott J. L., Davidson J. G., Marino J. V., McMillan R. Leukocyte labeling with 51 chromium. 3. The kinetics of normal lymphocytes. Blood. 1972 Aug;40(2):276–281. [PubMed] [Google Scholar]
  15. Segel G. B., Lichtman M. A., Gordon B. R., MacPherson J. L., Nusbacher J. Plateletpheresis residues: a source of large quantities of human blood lymphocytes. Transfusion. 1976 Sep-Oct;16(5):455–459. doi: 10.1046/j.1537-2995.1976.16577039302.x. [DOI] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES