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. 1981 Jul;45(1):143–151.

Histological and immunological investigation of liver-specific protein (LSP) immunized rabbits compared with patients with liver disease.

C Feighery, G S McDonald, J F Greally, D G Weir
PMCID: PMC1537239  PMID: 7307345

Abstract

In this study 45 patients with a variety of liver diseases did not demonstrate T lymphocyte sensitivity to liver-specific protein (LSP) as assessed by lymphocyte transformation whereas LSP-immunized rabbits developed both cellular and humoral immunity to this antigen. Although these LSP-immunized rabbits demonstrated portal tract inflammation with some hepatocyte necrosis, rabbits immunized with antigens known to contaminate LSP developed similar lesions. These findings contrast with those of other investigators who have reported immune responsiveness to LSP in liver patients and chronic active hepatitis in LSP-immunized rabbits. In determining the significance of these studies it must be emphasized that all preparations of LSP contain an heterogeneous group of antigens and that the specific sensitizing antigen has yet to be identified.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bloom B. R. In vitro approaches to the mechanism of cell-mediated immune reactions. Adv Immunol. 1971;13:101–208. doi: 10.1016/s0065-2776(08)60184-4. [DOI] [PubMed] [Google Scholar]
  2. Brostoff J., Roitt I. M., Doniach D. Leucocyte-migration inhibition in autoimmune diseases. Lancet. 1969 Jun 14;1(7607):1212–1213. doi: 10.1016/s0140-6736(69)92192-8. [DOI] [PubMed] [Google Scholar]
  3. Chisari F. V. Liver-specific protein in perspective. Gastroenterology. 1980 Jan;78(1):168–170. [PubMed] [Google Scholar]
  4. Cochrane A. M., Moussouros A., Portmann B., McFarlane I. G., Thomson A. D., Eddleston, Williams R. Lymphocyte cytotoxicity for isolated hepatocytes in alcoholic liver disease. Gastroenterology. 1977 May;72(5 Pt 1):918–923. [PubMed] [Google Scholar]
  5. Cochrane A. M., Moussouros A., Smith A., Thomson A. D., Eddleston A. L., Williams R. Autoimmune reaction to a liver specific membrane antigen during acute viral hepatitis. Gut. 1976 Sep;17(9):714–718. doi: 10.1136/gut.17.9.714. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cochrane A. M., Moussouros A., Thomsom A. D., Eddleston A. L., Wiiliams R. Antibody-dependent cell-mediated (K cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis. Lancet. 1976 Feb 28;1(7957):441–444. doi: 10.1016/s0140-6736(76)91472-0. [DOI] [PubMed] [Google Scholar]
  7. EMMELOT P., BOS C. J., BENEDETTI E. L., RUEMKE P. STUDIES ON PLASMA MEMBRANES. I. CHEMICAL COMPOSITION AND ENZYME CONTENT OF PLASMA MEMBRANES ISOLATED FROM RAT LIVER. Biochim Biophys Acta. 1964 Jul 15;90:126–145. doi: 10.1016/0304-4165(64)90125-4. [DOI] [PubMed] [Google Scholar]
  8. Feighery C., Weir D. G. How specific is liver-specific protein? Gastroenterology. 1980 Jul;79(1):179–180. [PubMed] [Google Scholar]
  9. Feighery C., Whelan C. A., Weir D. G., Greally J. F. In vitro studies of suppressor cell function in human peripheral blood mononuclear cells. Clin Exp Immunol. 1978 Jun;32(3):459–465. [PMC free article] [PubMed] [Google Scholar]
  10. Jensen D. M., McFarlane I. G., Portmann B. S., Eddleston A. L., Williams R. Detection of antibodies directed against a liver-specific membrane lipoprotein in patients with acute and chronic active hepatitis. N Engl J Med. 1978 Jul 6;299(1):1–7. doi: 10.1056/NEJM197807062990101. [DOI] [PubMed] [Google Scholar]
  11. Kakumu S., Arakawa Y., Goji H., Kashio T., Yata K. Occurrence and significance of antibody to liver-specific membrane lipoprotein by double-antibody immunoprecipitation method in sera of patients with acute and chronic liver diseases. Gastroenterology. 1979 Apr;76(4):665–672. [PubMed] [Google Scholar]
  12. Kawanishi H., MacDermott R. P. K-cell-mediated antibody-dependent cellular cytotoxicity in chronic active liver disease. Gastroenterology. 1979 Jan;76(1):151–158. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. McFarlane I. G., Wojcicka B. M., Williams R. Antigens of the human liver. Clin Exp Immunol. 1980 Apr;40(1):1–7. [PMC free article] [PubMed] [Google Scholar]
  15. McFarlane I. G., Wojcicka B. M., Zucker G. M., Eddleston A. L., Williams R. Purification and characterization of human liver-specific membrane lipoprotein (LSP). Clin Exp Immunol. 1977 Mar;27(3):381–390. [PMC free article] [PubMed] [Google Scholar]
  16. Meyer zum Büschenfelde K. H., Hopf U. Studies on the pathogenesis of experimental chronic active hepatitis in rabbits. I. Induction of the disease and protective effect of allogeneic liver specific proteins. Br J Exp Pathol. 1974 Oct;55(5):498–508. [PMC free article] [PubMed] [Google Scholar]
  17. Miller J., Smith M. G., Mitchell C. G., Reed W. D., Eddleston A. L., Williams R. Cell-mediated immunity to a human liver-specific antigen in patients with active chronic hepatitis and primary biliary cirrhosis. Lancet. 1972 Aug 12;2(7772):296–297. doi: 10.1016/s0140-6736(72)92904-2. [DOI] [PubMed] [Google Scholar]
  18. Mogensen C. E. The glomerular permeability determined by dextran clearance using Sephadex gel filtration. Scand J Clin Lab Invest. 1968;21(1):77–82. doi: 10.3109/00365516809076979. [DOI] [PubMed] [Google Scholar]
  19. Ohta G., Nonomura A., Nishimura I. Leucocyte migration inhibition with inner and outer membranes of mitochondria and insoluble hepatocyte surface membranes prepared from rat liver in patients with chronic hepatitis and cirrhosis. Clin Exp Immunol. 1976 Dec;26(3):491–504. [PMC free article] [PubMed] [Google Scholar]
  20. Ortona L., Laghi V., Cauda R., Nervo P. Lymphocyte transformation test with rabbit liver specific lipoprotein (RLSP) in chronic active hepatitis. Clin Exp Immunol. 1979 Nov;38(2):231–234. [PMC free article] [PubMed] [Google Scholar]
  21. Thestrup-Pedersen K., Ladefoged K., Andersen P. Lymphocyte transformation test with liver-specific protein and phytohaemagglutinin in patients with liver disease. Clin Exp Immunol. 1976 Apr;24(1):1–8. [PMC free article] [PubMed] [Google Scholar]
  22. Vogten A. J., Hadzic N., Shorter R. G., Summerskill W. H., Taylor W. F. Cell-mediated cytotoxicity in chronic active liver disease: a new test system. Gastroenterology. 1978 May;74(5 Pt 1):883–889. [PubMed] [Google Scholar]

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