Skip to main content
PMC home page
Clinical and Experimental Immunology logoLink to Clinical and Experimental Immunology
. 1981 Sep;45(3):604–614.

Lymphocyte traffic through chronic inflammatory lesions: differential migration versus differential retention.

T B Issekutz, G W Chin, J B Hay
PMCID: PMC1537409  PMID: 7337964

Abstract

Afferent lymphatics draining granulomas and efferent lymphatics from normal lymph nodes were cannulated in sheep. Cells collected from these lymphatics were radiolabelled in vitro with 111In (afferent lymph cells) and 51Cr (efferent lymphocytes) and both labelled cells were returned to the animal simultaneously by i.v. injection. The reappearance of these labelled cells in lymph, and the amount of 111In and 51Cr in normal or antigenically stimulated lymph nodes, cutaneous inflammatory sites (FCA-granulomas, NLT- and BCG-induced lesions) and blood was determined 24 hr later. As previously reported, labelled afferent cells preferentially migrated from the blood back through the granuloma into afferent lymph, and efferent lymphocytes back into efferent lymph. Forty per cent as many 111In- as 51Cr-labelled cells ;appeared in efferent lymph. This was caused by the greater migration of 51Cr-labelled cells appeared in efferent lymph. This was caused by the greater migration of 51Cr- than 111In-labelled cells out of the blood into the node. Neither cell type was selectively retained in the node, and 28% of the labelled cells that entered the node migrated on into efferent lymph in 24 hr. Similarly, there was no selective retention of either cell type in the granuloma, and equal amounts of 111In the 51Cr appeared in afferent lymph. The ratio 111In/51Cr in the blood suggested that in the lymph node the two labelled cell populations were extracted equally, while in the granuloma selectively at the level of the vascular endothelium resulted in the preferential extraction of 111In-labelled (afferent lymph) cells.

Full text

PDF
604

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Butcher E. C., Scollay R. G., Weissman I. L. Organ specificity of lymphocyte migration: mediation by highly selective lymphocyte interaction with organ-specific determinants on high endothelial venules. Eur J Immunol. 1980 Jul;10(7):556–561. doi: 10.1002/eji.1830100713. [DOI] [PubMed] [Google Scholar]
  2. Cahill R. N., Poskitt D. C., Frost D. C., Trnka Z. Two distinct pools of recirculating T lymphocytes: migratory characteristics of nodal and intestinal T lymphocytes. J Exp Med. 1977 Feb 1;145(2):420–428. doi: 10.1084/jem.145.2.420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chin W., Hay J. B. A comparison of lymphocyte migration through intestinal lymph nodes, subcutaneous lymph nodes, and chronic inflammatory sites of sheep. Gastroenterology. 1980 Dec;79(6):1231–1242. [PubMed] [Google Scholar]
  4. Claesson M. H., Jorgensen O., Ropke C. Light and electron microscopic studies of the paracortical post-capillary high-endothelial venules. Z Zellforsch Mikrosk Anat. 1971;119(2):195–207. doi: 10.1007/BF00324521. [DOI] [PubMed] [Google Scholar]
  5. Ford W. L. Lymphocyte migration and immune responses. Prog Allergy. 1975;19:1–59. doi: 10.1159/000313381. [DOI] [PubMed] [Google Scholar]
  6. Ford W. L., Simmonds S. J. The tempo of lymphocyte recirculation from blood to lymph in the rat. Cell Tissue Kinet. 1972 Mar;5(2):175–189. doi: 10.1111/j.1365-2184.1972.tb01014.x. [DOI] [PubMed] [Google Scholar]
  7. GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
  8. GOWANS J. L. The recirculation of lymphocytes from blood to lymph in the rat. J Physiol. 1959 Apr 23;146(1):54–69. doi: 10.1113/jphysiol.1959.sp006177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HALL J. G., MORRIS B. The output of cells in lymph from the popliteal node of sheep. Q J Exp Physiol Cogn Med Sci. 1962 Oct;47:360–369. doi: 10.1113/expphysiol.1962.sp001620. [DOI] [PubMed] [Google Scholar]
  10. Hay J. B., Hobbs B. B., Johnston M. G., Movat H. Z. The role of hyperemia in cellular hypersensitivity reactions. Int Arch Allergy Appl Immunol. 1977;55(1-6):324–331. doi: 10.1159/000231943. [DOI] [PubMed] [Google Scholar]
  11. Hay J. B., Hobbs B. B. The flow of blood to lymph nodes and its relation to lymphocyte traffic and the immune response. J Exp Med. 1977 Jan 1;145(1):31–44. doi: 10.1084/jem.145.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Issekutz T. B., Chin W., Hay J. B. Lymphocyte traffic through granulomas: differences in the recovery of indium-111-labeled lymphocytes in afferent and efferent lymph. Cell Immunol. 1980 Aug 15;54(1):79–86. doi: 10.1016/0008-8749(80)90191-4. [DOI] [PubMed] [Google Scholar]
  13. Issekutz T., Chin W., Hay J. B. Measurement of lymphocyte traffic with indium-111. Clin Exp Immunol. 1980 Jan;39(1):215–221. [PMC free article] [PubMed] [Google Scholar]
  14. LASCELLES A. K., MORRIS B. Surgical techniques for the collection of lymph from unanaesthetized sheep. Q J Exp Physiol Cogn Med Sci. 1961 Jul;46:199–205. doi: 10.1113/expphysiol.1961.sp001536. [DOI] [PubMed] [Google Scholar]
  15. Morris B., Courtice F. C. Cells and immunoglobulins in lymph. Lymphology. 1977 Jun;10(2):62–70. [PubMed] [Google Scholar]
  16. Schoefl G. I. The migration of lymphocytes across the vascular endothelium in lymphoid tissue. A reexamination. J Exp Med. 1972 Sep 1;136(3):568–588. doi: 10.1084/jem.136.3.568. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith J. B., McIntosh G. H., Morris B. The migration of cells through chronically inflamed tissues. J Pathol. 1970 Jan;100(1):21–29. doi: 10.1002/path.1711000104. [DOI] [PubMed] [Google Scholar]
  18. Smith J. B., McIntosh G. H., Morris B. The traffic of cells through tissues: a study of peripheral lymph in sheep. J Anat. 1970 Jul;107(Pt 1):87–100. [PMC free article] [PubMed] [Google Scholar]
  19. Sprent J. Circulating T and B lymphocytes of the mouse. I. Migratory properties. Cell Immunol. 1973 Apr;7(1):10–39. doi: 10.1016/0008-8749(73)90180-9. [DOI] [PubMed] [Google Scholar]
  20. Stamper H. B., Jr, Woodruff J. J. Lymphocyte homing into lymph nodes: in vitro demonstration of the selective affinity of recirculating lymphocytes for high-endothelial venules. J Exp Med. 1976 Sep 1;144(3):828–833. doi: 10.1084/jem.144.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Clinical and Experimental Immunology are provided here courtesy of British Society for Immunology

RESOURCES